公开(公告)号：US10358649B2

公开(公告)日：2019-07-23

申请号：US15466669

申请日：2017-03-22

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： JoAnne Fillatti ,  Larry Gilbertson ,  Toni Voelker

IPC: C12N15/82 ,  C12N15/63

Abstract: Methods of providing gene suppression DNA in a eukaryotic organism comprising introducing a first DNA segment and at least one second DNA segment into the genome of the organism. One of the DNA segments contains a promoter and a transcribable DNA. Another DNA segment contains at least part of the transcribable DNA. When inserted in tandem, the DNA segments are assembled in vivo forming a recombinant transcription unit. RNA transcribed from the transcription unit can form double-stranded RNA.

公开(公告)号：US20170191074A1

公开(公告)日：2017-07-06

申请号：US15466669

申请日：2017-03-22

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： JoAnne Fillatti ,  Larry Gilbertson ,  Toni Voelker

IPC: C12N15/82

CPC classification number: C12N15/8201 ,  C12N15/63 ,  C12N15/8203 ,  C12N15/8213 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8247

Abstract: Methods of providing gene suppression DNA in a eukaryotic organism comprising introducing a first DNA segment and at least one second DNA segment into the genome of the organism. One of the DNA segments contains a promoter and a transcribable DNA. Another DNA segment contains at least part of the transcribable DNA. When inserted in tandem, the DNA segments are assembled in vivo forming a recombinant transcription unit. RNA transcribed from the transcription unit can form double-stranded RNA.

公开(公告)号：US09611484B2

公开(公告)日：2017-04-04

申请号：US14109835

申请日：2013-12-17

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： JoAnne Fillatti ,  Larry Gilbertson ,  Toni Voelker

IPC: C12N15/82 ,  C12N15/63

CPC classification number: C12N15/8213 ,  C12N15/63 ,  C12N15/8201 ,  C12N15/8203 ,  C12N15/8205 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8247 ,  C12N15/8261

Abstract: Methods of providing gene suppression DNA in a eukaryotic organism comprising introducing a first DNA segment and at least one second DNA segment into the genome of the organism. One of the DNA segments contains a promoter and a transcribable DNA. Another DNA segment contains at least part of the transcribable DNA. When inserted in tandem, the DNA segments are assembled in vivo forming a recombinant transcription unit. RNA transcribed from the transcription unit can form double-stranded RNA.

公开(公告)号：US20150247153A1

公开(公告)日：2015-09-03

申请号：US14430703

申请日：2013-09-24

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： JoAnne Fillatti ,  Byron Froman ,  Graeme S. Garvey ,  Johannes C. Hemmes

IPC: C12N15/82 ,  A01N25/00 ,  A01N57/16

CPC classification number: C12Q1/6895 ,  A01H5/12 ,  A01N25/00 ,  A01N57/16 ,  A01N65/12 ,  A23B7/155 ,  C12N9/0059 ,  C12N15/1137 ,  C12N15/8218 ,  C12N15/825 ,  C12N15/8261 ,  C12N15/8266 ,  C12Q1/686 ,  C12Y110/03001

Abstract: The present invention provides novel compositions for use to enhance crop performance. Specifically, the present invention provides for improved shelf life and reduced postharvest losses enhancement in various crops. The present invention also provides for combinations of compositions and methods that provide for improved shelf life and reduced postharvest losses.

Abstract translation: 本发明提供用于增强作物性能的新型组合物。 具体而言，本发明提供了改善的保存期限和减少各种作物的收获后损失。 本发明还提供组合物和方法的组合，其提供改善的保质期和降低的收获后损失。

公开(公告)号：US20140109260A1

公开(公告)日：2014-04-17

申请号：US14109835

申请日：2013-12-17

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： JoAnne Fillatti ,  Larry Gilbertson ,  Toni Voelker

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/63 ,  C12N15/8201 ,  C12N15/8203 ,  C12N15/8205 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8247 ,  C12N15/8261

Abstract: Methods of providing gene suppression DNA in a eukaryotic organism comprising introducing a first DNA segment and at least one second DNA segment into the genome of the organism. One of the DNA segments contains a promoter and a transcribable DNA. Another DNA segment contains at least part of the transcribable DNA. When inserted in tandem, the DNA segments are assembled in vivo forming a recombinant transcription unit. RNA transcribed from the transcription unit can form double-stranded RNA.

Abstract translation: 在真核生物体中提供基因抑制DNA的方法包括将第一DNA片段和至少一个第二DNA片段引入生物体的基因组。 其中一个DNA片段含有启动子和可转录的DNA。 另外DNA片段含有至少部分可转录的DNA。 当串联插入时，DNA片段在体内组装形成重组转录单元。 从转录单位转录的RNA可以形成双链RNA。