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    In vivo Assembly of Transcription Units
    3.
    发明申请
    In vivo Assembly of Transcription Units 审中-公开
    转录单位的体内装配

    公开(公告)号：US20140109260A1

    公开(公告)日：2014-04-17

    申请号：US14109835

    申请日：2013-12-17

    Applicant: MONSANTO TECHNOLOGY LLC

    Inventor： JoAnne Fillatti ,  Larry Gilbertson ,  Toni Voelker

    IPC: C12N15/82

    CPC classification number: C12N15/8213 ,  C12N15/63 ,  C12N15/8201 ,  C12N15/8203 ,  C12N15/8205 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8247 ,  C12N15/8261

    Abstract: Methods of providing gene suppression DNA in a eukaryotic organism comprising introducing a first DNA segment and at least one second DNA segment into the genome of the organism. One of the DNA segments contains a promoter and a transcribable DNA. Another DNA segment contains at least part of the transcribable DNA. When inserted in tandem, the DNA segments are assembled in vivo forming a recombinant transcription unit. RNA transcribed from the transcription unit can form double-stranded RNA.

    Abstract translation: 在真核生物体中提供基因抑制DNA的方法包括将第一DNA片段和至少一个第二DNA片段引入生物体的基因组。 其中一个DNA片段含有启动子和可转录的DNA。 另外DNA片段含有至少部分可转录的DNA。 当串联插入时，DNA片段在体内组装形成重组转录单元。 从转录单位转录的RNA可以形成双链RNA。

    In vivo assembly of transcription units
    8.
    发明授权
    In vivo assembly of transcription units 有权

    公开(公告)号：US10358649B2

    公开(公告)日：2019-07-23

    申请号：US15466669

    申请日：2017-03-22

    Applicant: MONSANTO TECHNOLOGY LLC

    Inventor： JoAnne Fillatti ,  Larry Gilbertson ,  Toni Voelker

    IPC: C12N15/82 ,  C12N15/63

    Abstract: Methods of providing gene suppression DNA in a eukaryotic organism comprising introducing a first DNA segment and at least one second DNA segment into the genome of the organism. One of the DNA segments contains a promoter and a transcribable DNA. Another DNA segment contains at least part of the transcribable DNA. When inserted in tandem, the DNA segments are assembled in vivo forming a recombinant transcription unit. RNA transcribed from the transcription unit can form double-stranded RNA.

    METHODS AND COMPOSITIONS FOR EXPRESSION OF TRANSGENES IN PLANTS
    9.
    发明申请
    METHODS AND COMPOSITIONS FOR EXPRESSION OF TRANSGENES IN PLANTS 审中-公开
    用于表达植物中转基因的方法和组合物

    公开(公告)号：US20150040270A1

    公开(公告)日：2015-02-05

    申请号：US14516483

    申请日：2014-10-16

    Applicant: Monsanto Technology LLC

    Inventor： Larry Gilbertson ,  Jason Ward ,  Jianping Xu ,  Jon Lamb

    IPC: C12N15/82

    CPC classification number: C12N15/8261 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8216

    Abstract: Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.

    Abstract translation: 提供包含单个基因座中包含的多个转基因的转基因植物。 在某些方面，可以从第一位点表达7个或更多个转基因。 提供了用于转化具有多个转基因的植物细胞的方法。 还提供了用于表达和增强植物中一种或多种转基因表达的方法。

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