公开(公告)号：US20220404357A1

公开(公告)日：2022-12-22

申请号：US17824643

申请日：2022-05-25

Applicant: Montana Molecular LLC

Inventor： Thomas E. HUGHES ,  Paul H. TEWSON ,  Anne Marie QUINN

IPC: G01N33/566 ,  G01N33/542 ,  G01N33/58 ,  C07K14/00

Abstract: Described herein are novel fluorescent sensors for cyclic adenosine monophosphate (cAMP) that are based on single fluorescent proteins. These sensors use less visible spectrum than FRET-based sensors, produce robust changes in fluorescence, and can be combined with one another, or with other sensors, in a multiplex assay on standard fluorescent plate readers or live cell imaging systems.

公开(公告)号：US20170198364A1

公开(公告)日：2017-07-13

申请号：US15407111

申请日：2017-01-16

Applicant: MONTANA MOLECULAR LLC

Inventor： Thomas E. HUGHES ,  Paul H. TEWSON ,  Anne Marie QUINN

IPC: C12Q1/68 ,  G01N33/50 ,  G01N33/92 ,  C07K14/435 ,  C12N9/12

CPC classification number: C12Q1/6897 ,  C07K14/00 ,  C07K14/43595 ,  C07K2319/60 ,  C12N9/12 ,  C12N9/1205 ,  C12Y207/11013 ,  G01N33/502 ,  G01N33/5041 ,  G01N33/582 ,  G01N33/92 ,  G01N2333/43595 ,  G01N2333/912 ,  G01N2333/916

Abstract: Described herein are novel fluorescent sensors for Diacyl Glycerol (DAG) and phosphatidylinositol 4,5-bisphosphate (PIP2) that are based on circularly permuted fluorescent proteins. These sensors use less visible spectrum than FRET-based sensors, produce robust changes in fluorescence, and can be combined with one another, or with other sensors, in a multiplex assay on standard fluorescent plate readers or live cell imaging systems.

公开(公告)号：US20160274109A1

公开(公告)日：2016-09-22

申请号：US15033147

申请日：2014-11-04

Applicant: MONTANA MOLECULAR LLC

Inventor： Thomas E. HUGHES ,  Paul H. TEWSON ,  Anne Marie QUINN

IPC: G01N33/566 ,  C07K14/00

CPC classification number: G01N33/566 ,  C07K14/00 ,  C07K2319/60 ,  G01N33/542 ,  G01N33/582 ,  G01N33/6872

Abstract: Described herein are novel fluorescent sensors for cyclic adenosine monophosphate (cAMP) that are based on single fluorescent proteins. These sensors use less visible spectrum than FRET-based sensors, produce robust changes in fluorescence, and can be combined with one another, or with other sensors, in a multiplex assay on standard fluorescent plate readers or live cell imaging systems.

Abstract translation: 本文描述了基于单一荧光蛋白的环状腺苷一磷酸（cAMP）的新型荧光传感器。 这些传感器使用比基于FRET的传感器更少的可见光谱，产生强烈的荧光变化，并且可以在标准荧光板读数器或活细胞成像系统的多重测定中彼此或与其他传感器组合。