公开(公告)号：US09535069B2

公开(公告)日：2017-01-03

申请号：US14933213

申请日：2015-11-05

Applicant: MYTECH CO., LTD.

Inventor： Hiroaki Ito ,  Yuki Hasegawa ,  Katsuyuki Hasegawa

IPC: G01J3/44 ,  G01N33/574 ,  G01N33/543 ,  G01N33/553 ,  G01N21/65

CPC classification number: G01N21/658 ,  C01G5/00 ,  C01P2002/60 ,  G01N33/5438 ,  G01N33/553 ,  G01N33/57496

Abstract: A method for measuring cancer related substances including cancer cell-derived free DNA by Raman spectroscopy, involving steps for preparing a biochip having a meso-crystal region of silver oxides containing a silver peroxide, adding a blood serum or a biological sample solution dropwise onto the meso-crystal region of said biochip, selectively trapping the cancer-related substances having a positive charge in the sample, irradiating the trapped cancer-related substance with an exciting laser light and detecting a surface enhanced Raman scattering therefrom, wherein cancer diseases are evaluated on the basis of the intensity of the Surface Enhance Raman Scattering (SERS). In the carbon-specific D band and G band in the Raman scattering spectrum, a characteristic peak spectrum of the cancer-related substance can be detected in the proximity of the methyl group characteristic of 2900 cm−1.

Abstract translation: 一种通过拉曼光谱测定癌症相关物质（包括癌细胞衍生的游离DNA）的方法，包括制备具有含有过氧化银的氧化银的内消旋晶体区域的生物芯片的步骤，将血清或生物样品溶液滴加到 所述生物芯片的中观晶体区域，选择性地捕获样品中具有正电荷的癌症相关物质，用激发激光照射被捕获的癌症相关物质，并从其中检测表面增强的拉曼散射，其中评估癌症疾病 表面增强拉曼散射强度的基础（SERS）。 在拉曼散射光谱中的碳特异性D带和G带中，可以在特征2900cm -1附近检测癌相关物质的特征峰光谱。

公开(公告)号：US20160187344A1

公开(公告)日：2016-06-30

申请号：US14933213

申请日：2015-11-05

Applicant: Hiroaki Ito ,  MYTECH CO., LTD.

Inventor： Hiroaki Ito ,  Yuki Hasegawa ,  Katsuyuki Hasegawa

IPC: G01N33/574 ,  G01N21/65

CPC classification number: G01N21/658 ,  C01G5/00 ,  C01P2002/60 ,  G01N33/5438 ,  G01N33/553 ,  G01N33/57496

Abstract: A method for measuring cancer related substances including cancer cell-derived free DNA by Raman spectroscopy, involving steps for preparing a biochip having a meso-crystal region of silver oxides containing a silver peroxide, adding a blood serum or a biological sample solution dropwise onto the meso-crystal region of said biochip, selectively trapping the cancer-related substances having a positive charge in the sample, irradiating the trapped cancer-related substance with an exciting laser light and detecting a surface enhanced Raman scattering therefrom, wherein cancer diseases are evaluated on the basis of the intensity of the Surface Enhance Raman Scattering (SERS). In the carbon-specific D band and G band in the Raman scattering spectrum, a characteristic peak spectrum of the cancer-related substance can be detected in the proximity of the methyl group characteristic of 2900 cm−1.

Abstract translation: 一种通过拉曼光谱测定癌症相关物质（包括癌细胞衍生的游离DNA）的方法，包括制备具有含有过氧化银的氧化银的内消旋晶体区域的生物芯片的步骤，将血清或生物样品溶液滴加到 所述生物芯片的中观晶体区域，选择性地捕获样品中具有正电荷的癌症相关物质，用激发激光照射被捕获的癌症相关物质，并从其中检测表面增强的拉曼散射，其中评估癌症疾病 表面增强拉曼散射强度的基础（SERS）。 在拉曼散射光谱中的碳特异性D带和G带中，可以在特征2900cm -1附近检测癌相关物质的特征峰光谱。