公开(公告)号：US20170115216A1

公开(公告)日：2017-04-27

申请号：US15346078

申请日：2016-11-08

Applicant: MYTECH CO., LTD.

Inventor： Yuki HASEGAWA ,  Katsuyuki HASEGAWA

IPC: G01N21/552 ,  G01N33/49

CPC classification number: G01N21/554 ,  G01N21/6458 ,  G01N21/648 ,  G01N21/6486 ,  G01N21/658 ,  G01N33/49

Abstract: A cancer-related substance in blood or a biological sample can be detected in a selective manner, so it becomes possible to determine the occurrence of cancer by observation of fluorescent image of a crystal of the censor related substance or a coagulated state of the crystal on a plasmonic chip. In addition, the state of chemical modification of a histone tail can be determined by a Raman spectrum analysis. Furthermore, the location of the cancer-related substance aggregated on a substrate cannot be determined with naked eyes. Then, as a second aspect of the present invention, a method for diagnosing a cancer disease is provided, said method being characterized by firstly identifying the location of the region of a crystal by observing fluorescent image on a microscope, and then irradiating the crystal, with laser beam to analyze with respect to the chemical modification of a histone tail and a remodeling factor.

公开(公告)号：US20210341468A1

公开(公告)日：2021-11-04

申请号：US17096678

申请日：2020-11-12

Applicant: MYTECH CO. LTD.

Inventor： Yuki Hasegawa ,  Katsuyuki Hasegawa

IPC: G01N33/543 ,  G01N33/553 ,  G01N33/569 ,  G01N33/58

Abstract: The present invention relates to a system capable of performing simple and rapid inspection of an antigen equivalent to the immune chromatographic method with accuracy good as a PCR method. An embodiment relates to a novel fluorescence counting system for quantifying viruses or antibodies in an analyte which comprises an unit of providing an antigen or antibody phase solidified substrate by an aggregation method with quantum crystals, an unit for making a labeling liquor and labeling a virus or an antibody to be measured in the analyte by an antigen-antibody method, an unit of exciting the fluorescently labeled virus or antibody by a surface plasmon excitation method, and an unit of counting fluorescent points in an excited fluorescent screen to quantify the virus or antibody in the analyte.

公开(公告)号：US10365222B2

公开(公告)日：2019-07-30

申请号：US15314996

申请日：2014-05-08

Applicant: MYTECH CO., LTD.

Inventor： Yuki Hasegawa ,  Katsuyuki Hasegawa

IPC: G01N21/65 ,  C01G5/00 ,  G01N33/553 ,  G01N33/543 ,  G01N33/574

Abstract: Object: To provide a biochip for use in exhaustive analysis of a particular protein including DNA (deoxyribose nucleic acid) in a body fluid through Raman quantitative analysis.Resolving Means: Aqueous solution of metal complexes including plasmon metal selected from the group consisting of Au, Ag, Pt and Pd is supplied dropwise onto a carrier metal having an electrode potential of metal less noble than complex metal, followed by precipitation of nanometric quantum crystals from the metal complex on the carrier metal, the metal complex being so selected as to have a complex stability constant (log β) that is expressed by the following equation (I) correlating with the electrode potential E of the carrier metal: E°=(RT/|Z|·F)In(βi)  (I) (wherein E° represents the standard electrode potential, R represents a gas constant, T represents the absolute temperature, Z represents the ion valency, and F represents the Faraday constant), the surface property of the metal complex quantum crystals on the carrier metal being subsequently adjusted in dependence on an object to be detected in the aqueous solution prior to the precipitation or after the precipitation.

公开(公告)号：US20230050766A1

公开(公告)日：2023-02-16

申请号：US17788852

申请日：2020-12-25

Applicant: MYTECH CO., LTD.

Inventor： Yuki HASEGAWA ,  Katsuyuki HASEGAWA

IPC: C12Q1/68 ,  G01N21/64 ,  G01N33/483

Abstract: [Problem] Provided is a method for detecting of an autofluorescence Liquid Biopsy of Methylated Fragmented DNA (fragmented nucleosome) released into the blood by cell apoptosis as a disease-related substance
[Solution] The inventive method comprises a) a step of capturing the fragmented DNA (fragmented nucleosome) in the analyte as a disease-related substance onto the plasmonic metal meso-crystals; b) a step of irradiating the captured fragmented DNA (fragmented nucleosome) on the plasmonic metal meso-crystal with excitation light to enhance the autofluorescence by the surface plasmon enhancing effect, and acquiring a fluorescent colony image via a filter in a longer wavelength range than the excitation light filter; c) a step of adopting a pixel that exhibits a brightness greater than or equal to a predetermined threshold value of said fluorescent colony image; d) calculating a ratio of a total area value of pixels greater than or equal to a predetermined threshold value of a different two-wavelength region of the adopted measurement region.

公开(公告)号：US10215700B2

公开(公告)日：2019-02-26

申请号：US15346078

申请日：2016-11-08

Applicant: MYTECH CO., LTD.

Inventor： Yuki Hasegawa ,  Katsuyuki Hasegawa

IPC: G01N21/47 ,  G01N21/552 ,  G01N33/49 ,  G01N21/64 ,  G01N21/65

Abstract: A cancer-related substance in blood or a biological sample can be detected in a selective manner, so it becomes possible to determine the occurrence of cancer by observation of fluorescent image of a crystal of the censor related substance or a coagulated state of the crystal on a plasmonic chip. In addition, the state of chemical modification of a histone tail can be determined by a Raman spectrum analysis. Furthermore, the location of the cancer-related substance aggregated on a substrate cannot be determined with naked eyes. Then, as a second aspect of the present invention, a method for diagnosing a cancer disease is provided, said method being characterized by firstly identifying the location of the region of a crystal by observing fluorescent image on a microscope, and then irradiating the crystal, with laser beam to analyze with respect to the chemical modification of a histone tail and a remodeling factor.

公开(公告)号：US20170354950A1

公开(公告)日：2017-12-14

申请号：US15182568

申请日：2016-06-14

Applicant: MYTECH CO., LTD.

Inventor： Yuki HASEGAWA ,  Katsuyuki HASEGAWA

IPC: B01J20/06 ,  G01N33/483 ,  C01G5/00 ,  B01J20/32 ,  H01L31/032 ,  G01N21/65

CPC classification number: B01J20/06 ,  B01J20/3234 ,  C01G5/00 ,  C01P2002/85 ,  C01P2004/03 ,  C01P2004/30 ,  C01P2006/16 ,  C01P2006/40 ,  G01N21/658 ,  G01N33/4833 ,  G01N2333/435 ,  H01L31/032

Abstract: A method for preparing a meso crystal of silver oxide containing silver peroxide is provided. A quantum crystal of silver thiosulfate complex on a substrate or a particle made of copper metal or copper alloy is subjected to treating by an alkaline aqueous solution containing halogen ion to obtain a meso crystal of silver oxide containing the silver peroxide. The meso crystal of silver oxide having nanometer scale, containing a silver peroxide, the silver oxide nanocrystal being a superstructure three-dimensionally arranged in the shape of a neuron provided with properties being negatively charged in water and able to be reduced to a silver nanoparticle by a laser radiation.

公开(公告)号：US09535069B2

公开(公告)日：2017-01-03

申请号：US14933213

申请日：2015-11-05

Applicant: MYTECH CO., LTD.

Inventor： Hiroaki Ito ,  Yuki Hasegawa ,  Katsuyuki Hasegawa

IPC: G01J3/44 ,  G01N33/574 ,  G01N33/543 ,  G01N33/553 ,  G01N21/65

CPC classification number: G01N21/658 ,  C01G5/00 ,  C01P2002/60 ,  G01N33/5438 ,  G01N33/553 ,  G01N33/57496

Abstract: A method for measuring cancer related substances including cancer cell-derived free DNA by Raman spectroscopy, involving steps for preparing a biochip having a meso-crystal region of silver oxides containing a silver peroxide, adding a blood serum or a biological sample solution dropwise onto the meso-crystal region of said biochip, selectively trapping the cancer-related substances having a positive charge in the sample, irradiating the trapped cancer-related substance with an exciting laser light and detecting a surface enhanced Raman scattering therefrom, wherein cancer diseases are evaluated on the basis of the intensity of the Surface Enhance Raman Scattering (SERS). In the carbon-specific D band and G band in the Raman scattering spectrum, a characteristic peak spectrum of the cancer-related substance can be detected in the proximity of the methyl group characteristic of 2900 cm−1.

Abstract translation: 一种通过拉曼光谱测定癌症相关物质（包括癌细胞衍生的游离DNA）的方法，包括制备具有含有过氧化银的氧化银的内消旋晶体区域的生物芯片的步骤，将血清或生物样品溶液滴加到 所述生物芯片的中观晶体区域，选择性地捕获样品中具有正电荷的癌症相关物质，用激发激光照射被捕获的癌症相关物质，并从其中检测表面增强的拉曼散射，其中评估癌症疾病 表面增强拉曼散射强度的基础（SERS）。 在拉曼散射光谱中的碳特异性D带和G带中，可以在特征2900cm -1附近检测癌相关物质的特征峰光谱。

公开(公告)号：US20230243816A1

公开(公告)日：2023-08-03

申请号：US18159857

申请日：2023-01-26

Applicant: MYTECH CO. LTD.

Inventor： Yuki HASEGAWA ,  Katsuyuki HASEGAWA

IPC: G01N33/543 ,  G01N33/553 ,  G01N33/569

CPC classification number: G01N33/54306 ,  G01N33/553 ,  G01N33/56983 ,  G01N2333/165

Abstract: The present invention relates to a system capable of performing simple and rapid inspection of an antigen equivalent to the immune chromatographic method with accuracy good as a PCR method. An embodiment relates to a novel fluorescence counting system for quantifying viruses or antibodies in an analyte which comprises an unit of providing an antigen or antibody phase solidified substrate by an aggregation method with quantum crystals, an unit for making a labeling liquor and labeling a virus or an antibody to be measured in the analyte by an antigen-antibody method, an unit of exciting the fluorescently labeled virus or antibody by a surface plasmon excitation method, and an unit of counting fluorescent points in an excited fluorescent screen to quantify the virus or antibody in the analyte.

公开(公告)号：US11609229B2

公开(公告)日：2023-03-21

申请号：US17096678

申请日：2020-11-12

Applicant: MYTECH CO. LTD.

Inventor： Yuki Hasegawa ,  Katsuyuki Hasegawa

IPC: G01N33/543 ,  G01N33/553 ,  G01N33/569

Abstract: The present invention relates to a system capable of performing simple and rapid inspection of an antigen equivalent to the immune chromatographic method with accuracy good as a PCR method. An embodiment relates to a novel fluorescence counting system for quantifying viruses or antibodies in an analyte which comprises an unit of providing an antigen or antibody phase solidified substrate by an aggregation method with quantum crystals, an unit for making a labeling liquor and labeling a virus or an antibody to be measured in the analyte by an antigen-antibody method, an unit of exciting the fluorescently labeled virus or antibody by a surface plasmon excitation method, and an unit of counting fluorescent points in an excited fluorescent screen to quantify the virus or antibody in the analyte.

公开(公告)号：US20170248523A1

公开(公告)日：2017-08-31

申请号：US15314996

申请日：2014-05-08

Applicant: MYTECH CO., LTD.

Inventor： Yuki Hasegawa ,  Katsuyuki Hasegawa

IPC: G01N21/65 ,  C01G5/00 ,  G01N33/553 ,  G01N33/543 ,  G01N33/574

CPC classification number: G01N21/658 ,  C01G5/00 ,  C01P2002/60 ,  G01N33/5438 ,  G01N33/553 ,  G01N33/57496

Abstract: Object: To provide a biochip for use in exhaustive analysis of a particular protein including DNA (deoxyribose nucleic acid) in a body fluid through Raman quantitative analysis.Resolving Means: Aqueous solution of metal complexes including plasmon metal selected from the group consisting of Au, Ag, Pt and Pd is supplied dropwise onto a carrier metal having an electrode potential of metal less noble than complex metal, followed by precipitation of nanometric quantum crystals from the metal complex on the carrier metal, the metal complex being so selected as to have a complex stability constant (log β) that is expressed by the following equation (I) correlating with the electrode potential E of the carrier metal: E°=(RT/|Z|·F)In(βi)  (I) (wherein E° represents the standard electrode potential, R represents a gas constant, T represents the absolute temperature, Z represents the ion valency, and F represents the Faraday constant), the surface property of the metal complex quantum crystals on the carrier metal being subsequently adjusted in dependence on an object to be detected in the aqueous solution prior to the precipitation or after the precipitation.