公开(公告)号：US06558939B1

公开(公告)日：2003-05-06

申请号：US09551826

申请日：2000-04-17

申请人： Mads Nørregaard-Madsen ,  Peter Rahbek Østergaard ,  Claus Bo Vøge Christensen ,  Søren Flensted Lassen

发明人： Mads Nørregaard-Madsen ,  Peter Rahbek Østergaard ,  Claus Bo Vøge Christensen ,  Søren Flensted Lassen

IPC分类号： C12N956

CPC分类号： C12N9/54 ,  A61K38/00

摘要： Novel isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylase, peroxidases or oxidases.

摘要翻译： 与亲代RP-II蛋白酶，DNA构建体和编码所述蛋白酶和变体的表达的载体相比，RP-II型的新型分离的蛋白酶和RP-II蛋白酶的变体表现出改善的性质，能够表达蛋白酶的宿主细胞 和DNA构建体的变体，以及通过培养所述宿主细胞产生它们的方法。 蛋白酶可以有利地用作洗涤剂组合物和添加剂中的组分，任选地与其它酶如蛋白酶，脂肪酶，纤维素酶，淀粉酶，过氧化物酶或氧化酶组合使用。

公开(公告)号：US07217554B2

公开(公告)日：2007-05-15

申请号：US10639194

申请日：2003-08-11

申请人： Mads Nørregaard-Madsen ,  Peter Rahbek Østergaard ,  Claus Bo Vøge Christensen ,  Søren Flensted Lassen

发明人： Mads Nørregaard-Madsen ,  Peter Rahbek Østergaard ,  Claus Bo Vøge Christensen ,  Søren Flensted Lassen

IPC分类号： C12N9/50 ,  C12N15/57 ,  C12N15/74 ,  C11D3/386

CPC分类号： C12N9/54 ,  A61K38/00

摘要： The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.

摘要翻译： 本发明涉及RP-II型分离的蛋白酶和与亲本RP-II蛋白酶相比表现出改进性质的RP-II蛋白酶的变体，DNA构建体和编码所述蛋白酶和变体的表达的载体，具有宿主细胞能力 从DNA构建体表达蛋白酶和变体，以及通过培养所述宿主细胞产生它们的方法。 蛋白酶可以有利地用作洗涤剂组合物和添加剂中的组分，任选地与其它酶如蛋白酶，脂肪酶，纤维素酶，淀粉酶，过氧化物酶或氧化酶组合使用。