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15 条记录 (耗时 0.035 秒)

    PROTEASES AND VARIANTS THEREOF
    1.
    发明申请
    PROTEASES AND VARIANTS THEREOF 失效
    保护者及其变体

    公开(公告)号：US20110117624A1

    公开(公告)日：2011-05-19

    申请号：US13014100

    申请日：2011-01-26

    申请人： Mads Norregaard-Madsen ,  Peter Rabbek Østergaard ,  Claus Bo Voge Christensen ,  Søren Flensted Lassen

    发明人： Mads Norregaard-Madsen ,  Peter Rabbek Østergaard ,  Claus Bo Voge Christensen ,  Søren Flensted Lassen

    IPC分类号： C12N9/08 ,  C12N9/56 ,  C12N9/20 ,  C12N9/26 ,  C12N9/42 ,  C12N9/02

    CPC分类号： C12N9/54 ,  A61K38/00 ,  C11D3/386 ,  Y02A50/473

    摘要： The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.

    摘要翻译： 本发明涉及RP-II型分离的蛋白酶和与亲本RP-II蛋白酶相比表现出改进性质的RP-II蛋白酶的变体，DNA构建体和编码所述蛋白酶和变体的表达的载体，具有宿主细胞能力 从DNA构建体表达蛋白酶和变体，以及通过培养所述宿主细胞产生它们的方法。 蛋白酶可以有利地用作洗涤剂组合物和添加剂中的组分，任选地与其它酶如蛋白酶，脂肪酶，纤维素酶，淀粉酶，过氧化物酶或氧化酶组合使用。

    Proteases and variants thereof
    2.
    发明授权
    Proteases and variants thereof 失效
    蛋白酶及其变体

    公开(公告)号：US07537922B2

    公开(公告)日：2009-05-26

    申请号：US11679371

    申请日：2007-02-27

    申请人： Mads Norregaard-Madsen ,  Peter Rahbek Østergaard ,  Claus Bo Voge Christensen ,  Søren Flensted Lassen

    发明人： Mads Norregaard-Madsen ,  Peter Rahbek Østergaard ,  Claus Bo Voge Christensen ,  Søren Flensted Lassen

    IPC分类号： C12N9/54 ,  C11D3/386 ,  C12N15/57 ,  C12N15/74 ,  C12N15/75

    CPC分类号： C12N9/54 ,  A61K38/00 ,  C11D3/386 ,  Y02A50/473

    摘要： The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.

    摘要翻译： 本发明涉及RP-II型分离的蛋白酶和与亲本RP-II蛋白酶相比表现出改进性质的RP-II蛋白酶的变体，DNA构建体和编码所述蛋白酶和变体的表达的载体，具有宿主细胞能力 从DNA构建体表达蛋白酶和变体，以及通过培养所述宿主细胞产生它们的方法。 蛋白酶可以有利地用作洗涤剂组合物和添加剂中的组分，任选地与其它酶如蛋白酶，脂肪酶，纤维素酶，淀粉酶，过氧化物酶或氧化酶组合使用。

    Proteases and variants thereof
    3.
    发明授权
    Proteases and variants thereof 失效
    蛋白酶及其变体

    公开(公告)号：US06558939B1

    公开(公告)日：2003-05-06

    申请号：US09551826

    申请日：2000-04-17

    申请人： Mads Nørregaard-Madsen ,  Peter Rahbek Østergaard ,  Claus Bo Vøge Christensen ,  Søren Flensted Lassen

    发明人： Mads Nørregaard-Madsen ,  Peter Rahbek Østergaard ,  Claus Bo Vøge Christensen ,  Søren Flensted Lassen

    IPC分类号： C12N956

    CPC分类号： C12N9/54 ,  A61K38/00

    摘要： Novel isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylase, peroxidases or oxidases.

    摘要翻译： 与亲代RP-II蛋白酶，DNA构建体和编码所述蛋白酶和变体的表达的载体相比，RP-II型的新型分离的蛋白酶和RP-II蛋白酶的变体表现出改善的性质，能够表达蛋白酶的宿主细胞 和DNA构建体的变体，以及通过培养所述宿主细胞产生它们的方法。 蛋白酶可以有利地用作洗涤剂组合物和添加剂中的组分，任选地与其它酶如蛋白酶，脂肪酶，纤维素酶，淀粉酶，过氧化物酶或氧化酶组合使用。

    Endoglucanases
    6.
    发明授权
    Endoglucanases 失效
    内切葡聚糖酶

    公开(公告)号：US06855531B2

    公开(公告)日：2005-02-15

    申请号：US10007521

    申请日：2001-12-10

    申请人： Torben Henriksen ,  Søren Flensted Lassen ,  Markus Sakari Kauppinen

    发明人： Martin Shulein

    IPC分类号： C12N15/09 ,  C05F11/08 ,  C05F17/00 ,  C07G99/00 ,  C11D3/386 ,  C12N1/19 ,  C12N9/42 ,  C12N15/00 ,  C12R1/645 ,  C12R1/69 ,  C12R1/77 ,  C12R1/80 ,  C12S3/04 ,  C12S11/00 ,  D06M13/02 ,  D06M13/322 ,  D06M13/46 ,  D06M16/00 ,  D06P5/02 ,  D06P5/15 ,  D21C5/00 ,  D21H11/16 ,  D21H11/20 ,  D21H17/00 ,  D21H21/10 ,  C07H21/04 ,  D05M16/00

    CPC分类号： C12N9/2437 ,  A61K38/00 ,  C05F11/08 ,  C05F17/0036 ,  C07K2319/00 ,  C11D3/386 ,  C12P19/14 ,  C12Y302/01004 ,  D06M16/003 ,  D06P5/02 ,  D06P5/158 ,  D21C5/005 ,  D21H17/005 ,  D21H21/10 ,  Y02P20/145 ,  Y02W30/43

    摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO:79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa 1   2   3   4   5   6   7   8   9 Xaa Cys Xaa Trp Xaa 10  11  12  13  14 and a second amino acid sequence having the following sequence Trp Cys Cys Xaa Cys (SEQ ID NO:80) 1   2   3   4   5 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very good in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

    摘要翻译： 本发明涉及酶制剂，其基本上由具有纤维素分解活性的酶组成，并且包含具有以下序列的第一氨基酸序列和具有以下序列的第二氨基酸序列，其中在第一序列的位置3，氨基酸为Trp ，Tyr或Phe; 在第一序列的位置4处，氨基酸是Trp，Tyr或Phe; 在第一序列的位置8，氨基酸是Arg，Lys或His; 在第一序列的第9,10,12和14位，第2个序列的第4位，氨基酸是20个天然存在的氨基酸残基中的任一个，条件是在第一个氨基酸序列 ，（i）当第12位的氨基残基为Ser时，第14位的氨基酸残基不为Ser，（ii）当第12位的氨基残基为Gly时，第14位的氨基酸残基为 Ala在工业应用中表现非常好，例如洗衣组合物，用于生物抛光新制造的纺织品，用于提供纤维素织物或服装的磨损外观以及纸浆处理。 此外，本发明涉及编码这些酶的DNA构建体，提供编码这种酶的基因的方法，生产酶的方法，含有这些酶的酶制剂，以及这些酶用于许多工业应用的用途。

    Endoglucanases
    8.
    发明授权
    Endoglucanases 有权
    内切葡聚糖酶

    公开(公告)号：US06387690B1

    公开(公告)日：2002-05-14

    申请号：US09229911

    申请日：1999-01-13

    申请人： Martin Schülein ,  Lene Nonboe Andersen ,  Søren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Ilum Nielsen ,  Michiko Ihara ,  Shinobu Takagi

    发明人： Martin Schülein ,  Lene Nonboe Andersen ,  Søren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Ilum Nielsen ,  Michiko Ihara ,  Shinobu Takagi

    IPC分类号： D06M1600

    CPC分类号： C12N9/2437 ,  A61K38/00 ,  C05F11/08 ,  C05F17/0036 ,  C07K2319/00 ,  C11D3/386 ,  C12P19/14 ,  C12Y302/01004 ,  D06M16/003 ,  D06P5/02 ,  D06P5/158 ,  D21C5/005 ,  D21H17/005 ,  D21H21/10 ,  Y02P20/145 ,  Y02W30/43

    摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence consisting of 14 amino acid residues having the following sequence Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1   2   3   4   5   6   7   8   9   10  11  12 Trp Xaa 13  14 and a second amino acid sequence consisting of 5 amino acid residues having the following sequence Trp Cys Cys Xaa Cys 1   2   3   4   5 wherein, in position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 8 of the first sequence, the amino acid is Arg, Lys or His; in position 9, 10, 12 and 14, respectively, of the first sequence, and in position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue in position 12 is Ser, then the amino acid residue in position 14 is not Ser, and (ii) when the amino residue in position 12 is Gly, then the amino acid residue in position 14 is not Ala, performs very good in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

    摘要翻译： 本发明涉及酶制剂，其基本上由具有纤维素分解活性的酶组成，并且包含由具有以下序列的14个氨基酸残基组成的第一氨基酸序列，以及由具有以下序列的5个氨基酸残基组成的第二个氨基酸序列，其中， 第一序列的第3位，氨基酸为Trp，Tyr或Phe; 在第一序列的位置4，氨基酸是Trp，Tyr或Phe; 在第一序列的位置8，氨基酸是Arg，Lys或His; 分别位于第一序列的第9,10,12和14位，在第二序列的位置4中，氨基酸是20个天然存在的氨基酸残基中的任一个，条件是在第一个氨基酸序列 ，（i）当位置12的氨基残基为Ser时，14位的氨基酸残基不为Ser，（ii）当位置12的氨基残基为Gly时，位置14的氨基酸残基为 Ala在工业应用中表现非常好，例如洗衣组合物，用于生物抛光新制造的纺织品，用于提供纤维素织物或服装的磨损外观以及纸浆处理。 此外，本发明涉及编码这些酶的DNA构建体，提供编码这种酶的基因的方法，生产酶的方法，含有这些酶的酶制剂，以及这些酶用于许多工业应用的用途。