公开(公告)号：US20230042171A1

公开(公告)日：2023-02-09

申请号：US17785488

申请日：2020-12-16

申请人： Manus Bio Inc.

发明人： Michelle N. GOETTGE ,  Ryan N. PHILIPPE ,  Ajikumar Parayil KUMARAN ,  Christine Nicole S. SANTOS ,  Jason Eric DONALD ,  Christopher FREI ,  Aaron LOVE ,  Kaitlin CHAMBERS ,  Joyce SAMSON ,  Christopher TOOMEY

IPC分类号： C12P17/02 ,  C12N9/02 ,  C12N9/14 ,  C12P19/18 ,  C12P19/56 ,  C12P5/00

摘要： The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog.V), Mogroside VI (Mog.VI), Iso-Mogroside V (Isomog.V), siamenoside, and glycosylation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog.V, Mog.VI, and Isomog.V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog.V, Mog.VI, and Isomog.V, including food products, beverages, oral care products, sweeteners, and flavoring products.