公开(公告)号：US20220090031A1

公开(公告)日：2022-03-24

申请号：US17495139

申请日：2021-10-06

申请人： Manus Bio Inc.

发明人： Jason Eric DONALD ,  Ajikumar Parayil KUMARAN ,  Aaron LOVE ,  Christopher TOOMEY ,  Christine Nicole S. SANTOS

IPC分类号： C12N9/10 ,  C12P19/56 ,  C12N15/52

摘要： In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3′ carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a “1-3 UGT.” In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure.

公开(公告)号：US20230042171A1

公开(公告)日：2023-02-09

申请号：US17785488

申请日：2020-12-16

申请人： Manus Bio Inc.

发明人： Michelle N. GOETTGE ,  Ryan N. PHILIPPE ,  Ajikumar Parayil KUMARAN ,  Christine Nicole S. SANTOS ,  Jason Eric DONALD ,  Christopher FREI ,  Aaron LOVE ,  Kaitlin CHAMBERS ,  Joyce SAMSON ,  Christopher TOOMEY

IPC分类号： C12P17/02 ,  C12N9/02 ,  C12N9/14 ,  C12P19/18 ,  C12P19/56 ,  C12P5/00

摘要： The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog.V), Mogroside VI (Mog.VI), Iso-Mogroside V (Isomog.V), siamenoside, and glycosylation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog.V, Mog.VI, and Isomog.V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog.V, Mog.VI, and Isomog.V, including food products, beverages, oral care products, sweeteners, and flavoring products.

公开(公告)号：US20240093258A1

公开(公告)日：2024-03-21

申请号：US18038067

申请日：2021-11-24

申请人： Manus Bio Inc.

发明人： Ajikumar PARAYIL KUMARAN ,  Christine Nicole S. SANTOS ,  Jason DONALD ,  Aaron LOVE ,  Yiying ZHENG ,  Adel GHADERI ,  Vineet SHASTRY ,  Lu CHEN ,  Christopher TOOMEY ,  Hannah LYNCH ,  Eric NIEMINEN

IPC分类号： C12P19/60 ,  C12N9/10 ,  C12N9/90

CPC分类号： C12P19/60 ,  C12N9/1051 ,  C12N9/90 ,  C12Y204/01017 ,  C12Y503/01009

摘要： In various aspects and embodiments, the present disclosure provides methods for making glycosylated products, as well as bacterial cells and uridine diphosphate (UDP)-dependent glycosyltransferase (UGT) enzymes useful for the same. In other aspects and embodiments, the disclosure provides methods for high yield and/or high purity recovery of such glycoside products from microbial cultures or cell free reactions. In various aspects and embodiments, the disclosure provides for whole cell bioconversion processes involving the glycosylation of a desired substrate, and/or the recovery of the glycosylated product at high yield and/or high purity.

公开(公告)号：US20210009968A1

公开(公告)日：2021-01-14

申请号：US16912374

申请日：2020-06-25

申请人： Manus Bio, Inc.

发明人： Jason Eric DONALD ,  Ajikumar Parayil KUMARAN ,  Aaron LOVE ,  Christopher TOOMEY ,  Christine Nicole S. SANTOS

IPC分类号： C12N9/10 ,  C12N15/52 ,  C12P19/56

摘要： In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3′ carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a “1-3 UGT.” In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure.