利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

2 条记录 (耗时 0.015 秒)

    Polypeptides derived from RNA polymerases and use thereof
    1.
    发明授权
    Polypeptides derived from RNA polymerases and use thereof 有权
    衍生自RNA聚合酶的多肽及其用途

    公开(公告)号：US07335471B2

    公开(公告)日：2008-02-26

    申请号：US10472146

    申请日：2002-03-19

    申请人： Jean Guillerez ,  Pascal Lopez ,  Marc Dreyfus

    发明人： Jean Guillerez ,  Pascal Lopez ,  Marc Dreyfus

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12N9/1247 ,  C12P21/00

    摘要： Mutant RNA polymerases of phagic origin in which the peptide chain is modified by substitution, deletion or addition of at least one amino acid, the modification having the effect of reducing the sensitivity of the RNA polymerases to the initial transcription sequence of the DNA sequence coding for the RNA, for a method for production of the RNA, or proteins coded by the RNA, from given nucleotide sequences, comprising a sequence of DNA coding for the RNA, the transcription of which is placed under the control of a promoter recognised by wild-type RNA polymerases and the mutant RNA polymerases as above. The method has a higher yield of RNA than the yield obtained when using the wild-type RNA polymerases in the presence of the same non-consensual ITS as that found in the sequence of DNA coding for the RNA.

    摘要翻译： 通过取代，缺失或添加至少一个氨基酸来修饰肽链的突变型RNA聚合酶，所述修饰具有降低RNA聚合酶对于编码DNA聚合酶的DNA序列的初始转录序列的敏感性的作用 RNA，用于生产RNA的方法或由RNA编码的蛋白质，从给定的核苷酸序列，包括编码RNA的DNA序列，其转录置于由野生型识别的启动子的控制下， RNA聚合酶和突变型RNA聚合酶。 该方法在使用野生型RNA聚合酶时在与编码RNA的DNA序列相同的非自愿ITS存在下获得的产量高于RNA产量。

    Mutant E. coli strains, and their use for producing recombinant polypeptides
    2.
    发明授权
    Mutant E. coli strains, and their use for producing recombinant polypeptides 有权
    突变大肠杆菌菌株及其用于产生重组多肽的用途

    公开(公告)号：US06632639B1

    公开(公告)日：2003-10-14

    申请号：US09762481

    申请日：2001-05-17

    申请人： Marc Dreyfus ,  Pascal Lopez

    发明人： Marc Dreyfus ,  Pascal Lopez

    IPC分类号： C12P2102

    CPC分类号： C12N9/22 ,  C12N15/70

    摘要： The invention concerns the use of Escherichia coli (E. coli) strains whereof the gene coding for the Rnase E comprises a mutation such that the enzyme produced when said mutated gene is expressed no longer has a degrading activity on mRNA, said mutation more significantly not affecting the growth of E. coli strains, for implementing a method for producing specific exogenous recombinant polypeptides.

    摘要翻译： 本发明涉及使用编码Rnase E的基因包含一个或多个氨基酸序列的大肠杆菌（Escherichia coli），大肠杆菌（Escherichia coli），大肠杆菌（Escherichia coli） 突变，使得当所述突变基因被表达时产生的酶不再具有对mRNA的降解活性，所述突变更显着地不影响 E的生长。 大肠杆菌 菌株，用于实施用于产生特异性外源重组多肽的方法。