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公开(公告)号：US20100311819A1
公开(公告)日：2010-12-09
申请号：US12778278
申请日：2010-05-12
申请人： Marc Van De Craen , Phuay-Yee Goh , Marc Georges Logghe , Yee-Ling Khu , Katherine Mortier , Thierry Andre Olivier Eddy Bogaert
发明人： Marc Van De Craen , Phuay-Yee Goh , Marc Georges Logghe , Yee-Ling Khu , Katherine Mortier , Thierry Andre Olivier Eddy Bogaert
CPC分类号： A01N57/16 , A01N63/02 , A01N63/04 , C07K14/37 , C12N15/113 , C12N15/8218 , C12N15/8282 , C12N2310/14
摘要： The present invention concerns methods for controlling fungus infestation via dsRNA mediated gene silencing, whereby the intact fungus cell(s) are contacted with a double-stranded RNA from outside the fungal cell(s) and whereby the double-stranded RNA is taken up by the intact fungal cell(s). In one particular embodiment, the methods of the invention are used to alleviate plants from fungus pests. Alternatively, the methods are used for treating and/or preventing fungal infestation on a substrate or a subject in need of such treatment and/or prevention. Suitable fungal target genes and fragments thereof, dsRNA constructs, recombinant constructs and compositions are disclosed.
摘要翻译： 本发明涉及通过dsRNA介导的基因沉默来控制真菌感染的方法，其中完整的真菌细胞与来自真菌细胞外的双链RNA接触，由此双链RNA被 完整的真菌细胞。 在一个具体实施方案中，本发明的方法用于减轻植物从真菌害虫中的减少。 或者，所述方法用于治疗和/或预防对需要这种治疗和/或预防的基质或受试者的真菌侵袭。 公开了合适的真菌靶基因及其片段，dsRNA构建体，重组构建体和组合物。
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公开(公告)号：US08865968B2
公开(公告)日：2014-10-21
申请号：US12778278
申请日：2010-05-12
申请人： Marc Van De Craen , Phuay-Yee Goh , Marc Georges Logghe , Yee-Ling Khu , Katherine An Hilde Mortier , Thierry Andre Oliver Eddy Bogaert
发明人： Marc Van De Craen , Phuay-Yee Goh , Marc Georges Logghe , Yee-Ling Khu , Katherine An Hilde Mortier , Thierry Andre Oliver Eddy Bogaert
CPC分类号： A01N57/16 , A01N63/02 , A01N63/04 , C07K14/37 , C12N15/113 , C12N15/8218 , C12N15/8282 , C12N2310/14
摘要： The present invention concerns methods for controlling fungus infestation via dsRNA mediated gene silencing, whereby the intact fungus cell(s) are contacted with a double-stranded RNA from outside the fungal cell(s) and whereby the double-stranded RNA is taken up by the intact fungal cell(s). In one particular embodiment, the methods of the invention are used to alleviate plants from fungus pests. Alternatively, the methods are used for treating and/or preventing fungal infestation on a substrate or a subject in need of such treatment and/or prevention. Suitable fungal target genes and fragments thereof, dsRNA constructs, recombinant constructs and compositions are disclosed.
摘要翻译： 本发明涉及通过dsRNA介导的基因沉默来控制真菌感染的方法，其中完整的真菌细胞与来自真菌细胞外的双链RNA接触，由此双链RNA被 完整的真菌细胞。 在一个具体实施方案中，本发明的方法用于减轻植物从真菌害虫中的减少。 或者，所述方法用于治疗和/或预防对需要这种治疗和/或预防的基质或受试者的真菌侵袭。 公开了合适的真菌靶基因及其片段，dsRNA构建体，重组构建体和组合物。
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公开(公告)号：US20060247197A1
公开(公告)日：2006-11-02
申请号：US11396926
申请日：2006-04-03
申请人： Marc Van De Craen , Phuay-Yee Goh , Marc Logghe , Yee-Ling Khu , Katherine Mortier , Thierry Bogaert
发明人： Marc Van De Craen , Phuay-Yee Goh , Marc Logghe , Yee-Ling Khu , Katherine Mortier , Thierry Bogaert
CPC分类号： A01N57/16 , A01N63/02 , A01N63/04 , C07K14/37 , C12N15/113 , C12N15/8218 , C12N15/8282 , C12N2310/14
摘要： The present invention concerns methods for controlling fungus infestation via dsRNA mediated gene silencing, whereby the intact fungus cell(s) are contacted with a double-stranded RNA from outside the fungal cell(s) and whereby the double-stranded RNA is taken up by the intact fungal cell(s). In one particular embodiment, the methods of the invention are used to alleviate plants from fungus pests. Alternatively, the methods are used for treating and/or preventing fungal infestation on a substrate or a subject in need of such treatment and/or prevention. Suitable fungal target genes and fragments thereof, dsRNA constructs, recombinant constructs and compositions are disclosed.
摘要翻译： 本发明涉及通过dsRNA介导的基因沉默来控制真菌感染的方法，其中完整的真菌细胞与来自真菌细胞外的双链RNA接触，由此双链RNA被 完整的真菌细胞。 在一个具体实施方案中，本发明的方法用于减轻植物从真菌害虫中的减少。 或者，所述方法用于治疗和/或预防对需要这种治疗和/或预防的基质或受试者的真菌侵袭。 公开了合适的真菌靶基因及其片段，dsRNA构建体，重组构建体和组合物。
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公开(公告)号：US20130160156A1
公开(公告)日：2013-06-20
申请号：US13702831
申请日：2011-06-08
IPC分类号： C12N15/82 , C12N15/113 , C07K14/375 , A01H5/04 , A01H5/10 , A01N57/16 , A01H1/06
CPC分类号： C12N15/8282 , A01H1/06 , A01H5/04 , A01H5/10 , A01N57/16 , C07K14/375 , C12N15/113 , C12N15/8218
摘要： The present invention concerns methods for controlling and/or preventing fungus infestation on a cell, organism, substrate or material via dsRNA mediated gene silencing. The methods of the invention are particularly used to alleviate pathogenic fungal infestation on plants, plant materials or seeds. Suitable fungal target genes and fragments thereof, expression cassettes, dsRNA molecules, host cells expressing the dsRNA, compositions and transgenic plants and plant cells are provided.
摘要翻译： 本发明涉及通过dsRNA介导的基因沉默来控制和/或预防细胞，生物体，底物或材料上的真菌侵袭的方法。 本发明的方法特别用于减轻对植物，植物材料或种子的致病真菌侵袭。 提供合适的真菌靶基因及其片段，表达盒，dsRNA分子，表达dsRNA的宿主细胞，组合物和转基因植物和植物细胞。
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