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    Methods of Producing Peptides in Plants and Peptides Produced Thereby
    3.
    发明申请
    Methods of Producing Peptides in Plants and Peptides Produced Thereby 有权
    在此产生的植物和肽中产生肽的方法

    公开(公告)号：US20110217766A1

    公开(公告)日：2011-09-08

    申请号：US13019819

    申请日：2011-02-02

    申请人： Marcia J. Kieliszewski ,  Jianfeng Xu ,  Gary D. Meyer

    发明人： Marcia J. Kieliszewski ,  Jianfeng Xu ,  Gary D. Meyer

    IPC分类号： C12N15/85

    CPC分类号： C07K14/415 ,  A61K38/27 ,  C07K14/56 ,  C07K14/61 ,  C07K14/765 ,  C12N15/62 ,  C12N15/8257 ,  C12P21/005

    摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion are present. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are also present.

    摘要翻译： 包括使用驱动植物中翻译后修饰的密码子的蛋白质工程化糖基化位点的重组蛋白质的植物表达产量增加到克隆基因或cDNA的方法，并且设计克隆的基因或cDNA以包含靶向基因的植物分泌信号序列 存在用于分泌的产品（蛋白质）。 该方法导致重组糖基化蛋白质产量增加。 也存在根据这些方法生产的蛋白质。

    METHODS OF PRODUCING PEPTIDES IN PLANTS AND PEPTIDES PRODUCED THEREBY
    5.
    发明申请
    METHODS OF PRODUCING PEPTIDES IN PLANTS AND PEPTIDES PRODUCED THEREBY 审中-公开
    在植物中生产肽的方法和生产的肽

    公开(公告)号：US20100028993A1

    公开(公告)日：2010-02-04

    申请号：US12122606

    申请日：2008-05-16

    申请人： Marcia J. Kieliszewski ,  Jianfeng Xu ,  Gary D. Meyer

    发明人： Marcia J. Kieliszewski ,  Jianfeng Xu ,  Gary D. Meyer

    IPC分类号： C12N15/74

    CPC分类号： C07K14/415 ,  A61K38/27 ,  C07K14/56 ,  C07K14/61 ,  C07K14/765 ,  C12N15/62 ,  C12N15/8257 ,  C12P21/005

    摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion are present. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are also present.

    摘要翻译： 包括使用驱动植物中翻译后修饰的密码子的蛋白质工程化糖基化位点的重组蛋白质的植物表达产量增加到克隆基因或cDNA的方法，并且设计克隆的基因或cDNA以包含靶向基因的植物分泌信号序列 存在用于分泌的产品（蛋白质）。 该方法导致重组糖基化蛋白质产量增加。 也存在根据这些方法生产的蛋白质。

    Methods of Predicting Hyp-Glycosylation Sites For Proteins Expressed and Secreted in Plant Cells, and Related Methods and Products
    6.
    发明申请
    Methods of Predicting Hyp-Glycosylation Sites For Proteins Expressed and Secreted in Plant Cells, and Related Methods and Products 审中-公开
    预测植物细胞表达和分泌的蛋白质的低糖基化位点的方法及相关方法和产品

    公开(公告)号：US20080242834A1

    公开(公告)日：2008-10-02

    申请号：US11995063

    申请日：2006-07-10

    申请人： Marcia J. Kieliszewski ,  Jianfeng Xu

    发明人： Marcia J. Kieliszewski ,  Jianfeng Xu

    IPC分类号： C07K2/00 ,  C12P21/00

    CPC分类号： C12N15/8257 ,  G16B30/00

    摘要： Proteins with Hyp-glycosylation are more likely to be secreted in plant cells at high levels than those without. Methods are disclosed for the prediction of Pro-hydroxylation and Hyp-glycosylationsites in proteins. Such methods can be used to identify (1) proteins which, without modification, are predisposed to develop Hyp-glycosylation, if expressed in plant cells, and (2) modifications (especially substitution mutations) which increase the propensity of a protein to develop Hyp-glycosylation, with a view to high level or increased secretion. It is also possible to determine empirically whether a particular protein will undergo Hyp-glycosylation suitable for the desired level of secretion in plant cells. Both modified proteins, and methods for the expression and secretion of predisposed and modified proteins, are claimed.

    摘要翻译： 具有低糖基化功能的蛋白质在植物细胞中比不含有高蛋白质的蛋白质更有可能分泌。 公开了用于预测蛋白质中的Pro-羟基化和Hyp-糖基化的方法。 这样的方法可用于鉴定（1）如果在植物细胞中表达，则不经修饰，易于发生Hyp-糖基化的蛋白质，和（2）增加蛋白质发展Hyp的倾向的修饰（特别是取代突变） 糖基化，以达到高水平或增加分泌。 也可以根据经验确定特定蛋白质是否将进行适合植物细胞中所需水平分泌的Hyp糖基化。 要求修饰的蛋白质，以及倾向和修饰的蛋白质的表达和分泌的方法。

    Pitch detection method and apparatus
    7.
    发明授权
    Pitch detection method and apparatus 有权
    间距检测方法和装置

    公开(公告)号：US09153245B2

    公开(公告)日：2015-10-06

    申请号：US12798715

    申请日：2010-04-09

    申请人： Fengyan Qi ,  Dejun Zhang ,  Lei Miao ,  Jianfeng Xu ,  Qing Zhang ,  Yang Gao

    发明人： Fengyan Qi ,  Dejun Zhang ,  Lei Miao ,  Jianfeng Xu ,  Qing Zhang ,  Yang Gao

    IPC分类号： G10L25/90 ,  G10L19/09

    CPC分类号： G10L25/90 ,  G10L19/09

    摘要： A pitch detection method and apparatus are disclosed. The method includes: performing pitch detection on an input signal in a signal domain, and obtaining a candidate pitch; performing linear prediction (LP) on the input signal, and obtaining an LP residual signal; setting a candidate pitch range that includes the candidate pitch; searching the candidate pitch range for the LP residual signal, and obtaining a selected pitch.

    摘要翻译： 公开了一种俯仰检测方法和装置。 该方法包括：对信号域中的输入信号执行音调检测，并获得候选音调; 对输入信号执行线性预测（LP），获得LP残差信号; 设置包括候选音调的候选音高范围; 搜索候选音调范围用于LP残差信号，并获得所选音调。

    Method, apparatus and system for linear prediction coding analysis
    8.
    发明授权
    Method, apparatus and system for linear prediction coding analysis 有权
    用于线性预测编码分析的方法，装置和系统

    公开(公告)号：US08812307B2

    公开(公告)日：2014-08-19

    申请号：US13228965

    申请日：2011-09-09

    申请人： Jianfeng Xu ,  Lei Miao ,  Fengyan Qi ,  Dejun Zhang ,  Qing Zhang

    发明人： Jianfeng Xu ,  Lei Miao ,  Fengyan Qi ,  Dejun Zhang ,  Qing Zhang

    IPC分类号： G10L19/00 ,  G10L19/022 ,  G10L19/04

    CPC分类号： G10L19/022 ,  G10L19/04

    摘要： The present invention relates to communication technologies and discloses a method, an apparatus and a system for Linear Prediction Coding (LPC) analysis to improve LPC prediction performance and simplify analysis operation. The method includes: obtaining signal feature information of at least one sample point of input signals; comparing and analyzing the signal feature information to obtain an analysis result; selecting a window function according to the analysis result to perform adaptive windowing for the input signals and obtain windowed signals; and processing the windowed signals to obtain an LPC coefficient for linear prediction. The embodiments of the present invention are applicable to LPC.

    摘要翻译： 本发明涉及通信技术，并且公开了一种用于线性预测编码（LPC）分析的方法，装置和系统，以提高LPC预测性能并简化分析操作。 该方法包括：获取输入信号的至少一个采样点的信号特征信息; 比较和分析信号特征信息以获得分析结果; 根据分析结果选择窗口功能，对输入信号进行自适应窗口化，获得窗口信号; 并处理窗口信号以获得用于线性预测的LPC系数。 本发明的实施例可应用于LPC。

    Methods of producing peptides/proteins in plants and peptides/proteins produced thereby
    10.
    发明申请
    Methods of producing peptides/proteins in plants and peptides/proteins produced thereby 审中-公开
    在植物中产生肽/蛋白质的方法和由此产生的肽/蛋白质

    公开(公告)号：US20060026719A1

    公开(公告)日：2006-02-02

    申请号：US11036256

    申请日：2005-01-14

    申请人： Marcia Kieliszewski ,  Jianfeng Xu

    发明人： Marcia Kieliszewski ,  Jianfeng Xu

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C07K14/42 ,  C12N15/82 ,  C12N5/04

    CPC分类号： C07K14/415 ,  A61K38/27 ,  C07K14/56 ,  C07K14/61 ,  C07K14/765 ,  C12N15/62 ,  C12N15/8257 ,  C12P21/005

    摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising: engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.

    摘要翻译： 提高重组蛋白质植物表达产量的方法，其包括：使用驱动植物翻译后修饰的密码子将糖基化位点工程化为克隆基因或蛋白质cDNA; 并对克隆的基因或cDNA进行工程以包含靶向分泌基因产物（蛋白质）的植物分泌信号序列。 该方法导致重组糖基化蛋白质产量增加。 公开了根据这些方法生产的蛋白质。