公开(公告)号：US20120003738A1

公开(公告)日：2012-01-05

申请号：US13164392

申请日：2011-06-20

申请人： Marco A. Costa ,  Masakazu Ishikawa ,  Takayuki Asahara ,  Haruchika Masuda

发明人： Marco A. Costa ,  Masakazu Ishikawa ,  Takayuki Asahara ,  Haruchika Masuda

IPC分类号： C12N5/078 ,  C12N5/02

CPC分类号： C12N5/0647 ,  C12N5/0692 ,  C12N2500/90 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/165 ,  C12N2501/727

摘要： The present invention provides a method for expanding and improving functional capacity of human adult-derived progenitor cells in vitro using a closed culture system. The present invention provides a favorable condition for cell therapy to promote tissue repair and organogenesis via vasculogenesis and angiogenesis in clinical settings. The proposed closed bag culture system for culturing hemangioblast comprises of, in one embodiment, a serum-free culture medium containing one or more factors selected from the group consisting of stem cell growth factor, interleukin-6, FMS-like tyrosine kinase 3, thrombopoietin, and vascular endothelial growth factor and a kit for the preparation of the serum-free culture medium and the like.

摘要翻译： 本发明提供使用封闭培养系统在体外扩增和提高人成人祖细胞的功能能力的方法。 本发明提供细胞治疗在临床环境中通过血管生成和血管发生促进组织修复和器官发生的有利条件。 所提出的用于培养成血管细胞培养的封闭袋培养系统包括在一个实施方案中，含有一种或多种选自干细胞生长因子，白细胞介素-6，FMS样酪氨酸激酶3，血小板生成素 ，和血管内皮生长因子和用于制备无血清培养基等的试剂盒。

公开(公告)号：US08492148B2

公开(公告)日：2013-07-23

申请号：US11884949

申请日：2006-02-22

申请人： Takayuki Asahara ,  Haruchika Masuda

发明人： Takayuki Asahara ,  Haruchika Masuda

IPC分类号： C12N5/00

CPC分类号： A61K35/44 ,  C12N5/0692 ,  C12N2500/90 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/15 ,  C12N2501/165 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2506/11

摘要： The present invention provides a method for expanding an endothelial progenitor cell in vitro. More particularly, the present invention provides a method for culturing a hemangioblast comprising incubating a hemangioblast in a serum-free culture medium containing one or more factors selected from the group consisting of stem cell growth factor, interleukin-6, FMS-like tyrosine kinase 3 and thrombopoietin, and a vascular endothelial cell produced by the method; and a serum-free culture medium containing one or more factors selected from the group consisting of stem cell growth factor, interleukin-6, FMS-like tyrosine kinase 3 ligand and thrombopoietin, and a kit for the preparation of the serum-free culture medium and the like.

摘要翻译： 本发明提供了一种在体外扩张内皮祖细胞的方法。 更具体地，本发明提供了一种培养成血成血管细胞的方法，包括在含有选自干细胞生长因子，白细胞介素-6，FMS样酪氨酸激酶3的一种或多种因子的无血清培养基中培养成血成血管细胞 和血小板生成素，以及通过该方法产生的血管内皮细胞; 和含有选自干细胞生长因子，白细胞介素-6，FMS样酪氨酸激酶3配体和血小板生成素的一种或多种因子的无血清培养基，以及用于制备无血清培养基的试剂盒 等等。

公开(公告)号：US20080166327A1

公开(公告)日：2008-07-10

申请号：US11884949

申请日：2006-02-22

申请人： Takayuki Asahara ,  Haruchika Masuda

发明人： Takayuki Asahara ,  Haruchika Masuda

IPC分类号： A61K35/00 ,  C12N5/06 ,  A61P43/00

CPC分类号： A61K35/44 ,  C12N5/0692 ,  C12N2500/90 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/15 ,  C12N2501/165 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2506/11

摘要： The present invention provides a method for expanding an endothelial progenitor cell in vitro. More particularly, the present invention provides a method for culturing a hemangioblast comprising incubating a hemangioblast in a serum-free culture medium containing one or more factors selected from the group consisting of stem cell growth factor, interleukin-6, FMS-like tyrosine kinase 3 and thrombopoietin, and a vascular endothelial cell produced by the method; and a serum-free culture medium containing one or more factors selected from the group consisting of stem cell growth factor, interleukin-6, FMS-like tyrosine kinase 3 and thrombopoietin, and a kit for the preparation of the serum-free culture medium and the like.

摘要翻译： 本发明提供了一种在体外扩张内皮祖细胞的方法。 更具体地，本发明提供了一种培养成血成血管细胞的方法，包括在含有选自干细胞生长因子，白细胞介素-6，FMS样酪氨酸激酶3的一种或多种因子的无血清培养基中培养成血成血管细胞 和血小板生成素，以及通过该方法产生的血管内皮细胞; 和含有选自干细胞生长因子，白介素-6，FMS样酪氨酸激酶3和血小板生成素的一种或多种因子的无血清培养基，以及用于制备无血清培养基的试剂盒和 类似。

公开(公告)号：US20080166751A1

公开(公告)日：2008-07-10

申请号：US11884931

申请日：2006-02-22

申请人： Takayuki Asahara ,  Haruchika Masuda

发明人： Takayuki Asahara ,  Haruchika Masuda

IPC分类号： C12Q1/02 ,  C12N5/02

CPC分类号： G01N33/5005 ,  C12N5/0692 ,  C12N2501/115 ,  C12N2501/165

摘要： The present invention provides a method of forming an EPC colony with good reproducibility, and a method of analyzing the dynamics of EPC differentiation in the body of patient. More specifically, the present invention provides a method of analyzing the dynamics of differentiation of endothelial progenitor cells, which includes culturing hemangioblasts in a semisolid medium containing a vascular endothelial growth factor and a basic fibroblast growth factor, and evaluating the mode of endothelial progenitor cell colony formation; a method of forming an endothelial progenitor cell colony which includes culturing hemangioblasts in a semisolid medium containing a vascular endothelial growth factor and a basic fibroblast growth factor; a semisolid medium containing a vascular endothelial growth factor and a basic fibroblast growth factor; and a kit for preparing the semisolid medium and the like.

摘要翻译： 本发明提供了一种形成具有良好重现性的EPC集落的方法，以及分析患者体内EPC分化动力学的方法。 更具体地，本发明提供了分析内皮祖细胞分化动力学的方法，其包括在含有血管内皮生长因子和碱性成纤维细胞生长因子的半固体培养基中培养成血管细胞，并评价内皮祖细胞集落的模式 形成; 形成内皮祖细胞集落的方法，其包括在含有血管内皮生长因子和碱性成纤维细胞生长因子的半固体培养基中培养成血成血管细胞; 含有血管内皮生长因子和碱性成纤维细胞生长因子的半固体培养基; 以及用于制备半固体培养基等的试剂盒。