公开(公告)号：US07049124B1

公开(公告)日：2006-05-23

申请号：US10009500

申请日：2000-06-06

申请人： Maria Kordowicz ,  Detlef Gussow ,  Uwe Hofmann ,  Tadeusz Pacuszka ,  Andrzej Gardas

发明人： Maria Kordowicz ,  Detlef Gussow ,  Uwe Hofmann ,  Tadeusz Pacuszka ,  Andrzej Gardas

IPC分类号： C12N9/26 ,  C12N9/88 ,  C12N15/56 ,  C12N15/66 ,  A61K38/47 ,  A61K38/51

CPC分类号： C12N9/2408 ,  A61K38/00

摘要： The present invention relates to the isolation, purification and characterization of a hyaluronidase which derives from the tropical leech Hirudinaria manillensis. Therefore, according to this invention, the enzyme was called “manillase”. The invention is furthermore concerned with the recombinant method of production of manillase which includes the disclosure of DNA and amino acid sequences as well as of expression vectors and host systems. Finally, the invention relates to the use of manillase for therapeutic purposes, for example, for the treatment of myocardial diseases, thrombotic events and tumors.

摘要翻译： 本发明涉及从热带水蛭Hirudinaria manillensis得到的透明质酸酶的分离，纯化和表征。 因此，根据本发明，酶被称为“芒硝酶”。 本发明进一步涉及重组芽胞杆菌的生产方法，其包括DNA和氨基酸序列以及表达载体和宿主系统的公开。 最后，本发明涉及用于治疗目的的芒硝酶的用途，例如用于治疗心肌疾病，血栓形成事件和肿瘤。