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公开(公告)号:US20120329097A1
公开(公告)日:2012-12-27
申请号:US13479001
申请日:2012-05-23
IPC分类号: C12P19/34
CPC分类号: C12P19/34 , C12N15/1096 , C12Q1/6846 , C12Q2525/179 , C12Q2525/143 , C12Q2521/107
摘要: The present invention provides improved methods for the amplification of nucleic acid molecules. Methods for amplifying target polynucleotides, including mRNA, using oligonucleotides, DNA and RNA polymerases are provided. The invention further provides compositions and kits for practicing the methods, as well as methods which use the amplification products.
摘要翻译: 本发明提供了用于扩增核酸分子的改进方法。 提供了使用寡核苷酸,DNA和RNA聚合酶扩增靶多核苷酸(包括mRNA)的方法。 本发明还提供了用于实施该方法的组合物和试剂盒,以及使用扩增产物的方法。
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公开(公告)号:US20090082215A1
公开(公告)日:2009-03-26
申请号:US12111745
申请日:2008-04-29
CPC分类号: C12Q1/6806 , C12N15/1096 , C12Q1/6809 , C12Q1/6837 , C12Q1/6886 , C12Q2600/158 , G06F19/20 , C12Q2527/125
摘要: Methods and compositions relating to the generation and use of gene expression data from tissue samples that have been fixed and embedded are provided. The data can electronically stored and implemented as well as used to augment diagnosis and treatment of diseases.
摘要翻译: 提供了与已经固定和嵌入的组织样品的基因表达数据的产生和使用相关的方法和组合物。 数据可以电子存储和实现,并用于增加疾病的诊断和治疗。
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