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    Coleopteran-toxic polypeptide compositions
    1.
    发明申请
    Coleopteran-toxic polypeptide compositions 失效
    鞘翅目毒性多肽组合物

    公开(公告)号：US20060253925A1

    公开(公告)日：2006-11-09

    申请号：US11485621

    申请日：2006-07-12

    申请人： Mark Rupar ,  William Donovan ,  Chih-Rei Chu ,  Elizabeth Pease ,  Yuping Tan ,  Annette Slaney ,  Thomas Malvar ,  James Baum

    发明人： Mark Rupar ,  William Donovan ,  Chih-Rei Chu ,  Elizabeth Pease ,  Yuping Tan ,  Annette Slaney ,  Thomas Malvar ,  James Baum

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N1/20 ,  C12N15/82 ,  C12N5/04 ,  C07K14/325

    CPC分类号： C07K14/325 ,  A01N63/02 ,  C12N15/8286 ,  Y02A40/162

    摘要： Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed δ-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.

    摘要翻译： 公开了新型杀虫多肽，以及包含这些多肽，其肽片段和特异性抗体的组合物。 还公开了载体，转化的宿主细胞和含有编码公开的δ-内毒素多肽的核酸片段的转基因植物。 还公开了鉴定相关多肽和多核苷酸的方法，制备和使用包含这些多核苷酸序列的转基因细胞的方法，以及用于控制昆虫种群的方法，例如科罗拉多马铃薯甲虫，南方玉米根虫和西方玉米根虫，并赋予 对植物对目标昆虫物种的抗性。

    Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and Ctenocephalides SPP.
    3.
    发明申请
    Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and Ctenocephalides SPP. 失效
    苏云金芽孢杆菌CryET29组合物对鞘翅目昆虫和Ctenocephalides SPP有毒。

    公开(公告)号：US20070163000A1

    公开(公告)日：2007-07-12

    申请号：US11712704

    申请日：2007-03-01

    申请人： Mark Rupar ,  William Donovan ,  Yuping Tan ,  Annette Slaney

    发明人： Mark Rupar ,  William Donovan ,  Yuping Tan ,  Annette Slaney

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04 ,  C07K14/325 ,  C12N1/21

    CPC分类号： A01N63/00 ,  C07K14/325 ,  C12N15/8286 ,  G01N2430/10 ,  Y02A40/162 ,  Y10S530/825

    摘要： Disclosed is a novel δ-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against coleopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneum). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.

    摘要翻译： 公开了一种名为CryET29的新型三角内含物，其表现出对iphonapteran昆虫具有杀虫活性，包括猫跳蚤幼虫（Ctenocephalides felis）以及鞘翅目昆虫，包括南方玉米根虫（Diabrotica undecimpunctata），西方玉米根虫 （D. virgifera），科罗拉多马铃薯甲虫（Leptinotarsa decemlineata），日本甲虫（Popillia japonica）和红粉甲虫（Tribolium castaneum））。 还公开了编码CryET29，重组载体，宿主细胞和包含cryET29 DNA片段的转基因植物的核酸片段。 公开了制备和使用所公开的蛋白质和核酸片段的方法以及用于在体内和体外检测cryET29和CryET29序列的测定和诊断试剂盒。