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1.发明申请CELL CULTIVATION AND PRODUCTION OF RECOMBINANT PROTEINS BY MEANS OF AN ORBITAL SHAKE BIOREACTOR SYSTEM WITH DISPOSABLE BAGS AT THE 1,500 LITER SCALE 审中-公开细胞培养和生产重组蛋白质的方法是通过在1,500 LITER SCALE上的具有可拆卸袋的ORBITAL SHAKE生物反应器系统公开(公告)号：US20090233334A1
公开(公告)日：2009-09-17
申请号：US12046374
申请日：2008-03-11
摘要： The present invention provides a novel method for culturing cells as well as a novel method for producing a recombinant protein by culturing cells at large scale (up to 1,500 L nominal volume and 750 L working volume), whereby an inflated bag provides a sterile, disposable cultivation chamber. The inflated bag is partially filled with liquid cultivation media and cells, and placed into a containment vessel. The containment vessel is positioned onto an orbitally shaken platform. The orbital shaking moves the containment vessel and thus the bag and induces thereby motion to the liquid contained therein (“shake mixing”). This motion (caused by orbital shaking) induces a dynamic force field that ensures cell suspension, bulk mixing, and oxygen transfer from the liquid surface to the respiring cells without damaging shear or foam generation.
摘要翻译： 本发明提供了一种培养细胞的新方法以及通过大规模培养细胞（达1,500L标称体积和750L工作体积）来生产重组蛋白的新方法，由此充气袋提供无菌的，一次性的 栽培室。 充气袋部分地装有液体培养基和细胞，并放置在容纳容器中。 安全壳位于轨道上摇摆的平台上。 轨道振荡使得容纳容器和袋子移动并引起其运动到其中容纳的液体（“混合”）。 这种运动（由轨道震荡引起）引起动态力场，确保细胞悬浮，本体混合和从液体表面到呼吸细胞的氧传递，而不损害剪切或泡沫产生。
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公开(公告)号：US20210332982A1
公开(公告)日：2021-10-28
申请号：US16855569
申请日：2020-04-22
申请人： Carlos DeJesus , Maria DeJesus
发明人： Carlos DeJesus , Maria DeJesus
摘要： A multiple burner cooking assembly for portable cooking includes a table and a plurality of burners that is each of the burners is coupled to the table for having a cooking pot positioned thereon for cooking. A plurality of conduits is each of the conduits is coupled to the table and each of the conduits is fluidly coupled to a respective one of the burners. A plurality of valves is each fluidly coupled to a respective one of the conduits and each of the valves is in fluid communication with a respective one of the burners. A supply hose is fluidly coupled to the plurality of conduits and the supply hose is attachable to a fuel source to deliver the fuel in the fuel source to the conduits.
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