公开(公告)号：US20050003999A9

公开(公告)日：2005-01-06

申请号：US10291860

申请日：2002-11-08

申请人： Martin Davis ,  Anand Rao ,  Sylvie Gauthier ,  Yves Pouliot ,  Line Gourley ,  Anne-Francoise Allain

发明人： Martin Davis ,  Anand Rao ,  Sylvie Gauthier ,  Yves Pouliot ,  Line Gourley ,  Anne-Francoise Allain

IPC分类号： A23J3/34 ,  A61K38/55 ,  C12P21/06 ,  A61K38/17

CPC分类号： C12P21/06 ,  A23J3/343 ,  A61K38/556

摘要： Enzymatic digests of whey protein concentrates were prepared using animal, bacterial and fungal proteases, and evaluated for antihypertensive activities. ACE-inhibitory activity and antihypertension activity were obtained with a hydrolysate of whey protein isolate prepared with a porcine trypsin.

摘要翻译： 使用动物，细菌和真菌蛋白酶制备乳清蛋白浓缩物的酶消化，并评估抗高血压活性。 用猪胰蛋白酶制备的乳清蛋白分离物的水解产物获得ACE抑制活性和抗高血压活性。

公开(公告)号：US06998259B1

公开(公告)日：2006-02-14

申请号：US09702068

申请日：2000-10-30

申请人： Martin E. Davis ,  Anand Rao ,  Sylvie Gauthier ,  Yves Pouliot ,  Line Gourley ,  Anne-Francoise Allain

发明人： Martin E. Davis ,  Anand Rao ,  Sylvie Gauthier ,  Yves Pouliot ,  Line Gourley ,  Anne-Francoise Allain

IPC分类号： C12P21/06 ,  C12N9/60 ,  A01N37/18

CPC分类号： C12P21/06 ,  A23L33/18 ,  A23L33/19 ,  A23V2002/00 ,  A61K35/20 ,  A61K38/018 ,  A61K38/556 ,  A23V2200/326 ,  A23V2250/54252

摘要： Enzymatic digests of whey protein concentrates were prepared using animal, bacterial and fungal proteases, and evaluated for antihypertensive activities. The highest ACE-inhibitory activity was obtained with the purified peptide β-1g (f142–148) obtained by chemical synthesis, for which an IC50 value of 0.04 mg powder.ml−1 was found. The hydrolysates derived from BiPRO™ whey protein isolate and β-1g both gave higher antihypertensive activities (IC50 values of 0.29 to 0.90 mg powder.ml−1) than the other hydrolysates tested (IC50 values of 0.96 and 1.30 mg powder.ml−1). The recovered hydrolysate can be used to treat hypertension in mammals such as humans and domestic pets such as dogs and cats.

摘要翻译： 使用动物，细菌和真菌蛋白酶制备乳清蛋白浓缩物的酶消化，并评估抗高血压活性。 通过化学合成获得的纯化肽β-1g（f142-148）获得最高的ACE抑制活性，其中0.04mg粉末的IC 50值< / SUP>被发现。 衍生自BiPRO TM乳清蛋白分离物和β-1g的水解产物都具有较高的抗高血压活性（0.29至0.90mg粉末，比例≤0.1）比 测试其它水解产物（IC 50 S值0.96和1.30mg粉末，ml -1）。 回收的水解产物可用于治疗哺乳动物如人和宠物如狗和猫的高血压。