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1.发明申请公开(公告)号：US20050208659A1
公开(公告)日：2005-09-22
申请号：US10713008
申请日：2003-11-17
申请人： Masaaki Ikeda , Mimi Adachi
发明人： Masaaki Ikeda , Mimi Adachi
IPC分类号： A61K38/00 , C07K14/47 , C12N5/077 , C12N15/861 , C12N5/08
CPC分类号： C12N5/0657 , A61K38/00 , C07K14/4738 , C12N2510/00 , C12N2799/022 , C12N2799/06
摘要： Terminal differentiated cells are proliferated by introducing a cyclin and a cyclin dependent kinase into the nucleus of terminal differentiated cells, and then cultivating or holding the cells. A method for proliferating terminal differentiated cells comprising adding a nucleotide sequence coding for a nuclear localization signal to at least one of a cyclin gene and a cyclin dependent kinase gene, and introducing each of the genes to terminal differentiated cells in vitro, and then cultivating the cells, or introducing each of the genes directly to terminal differentiated cells in vivo is provided. The cyclin is a cyclin that can activate CDK4 or CDK6, and the cyclin dependent kinase is a cyclin dependent kinase that is activated by D-type cyclin. The invention also provides a recombinant vector used for such a method or a pharmaceutical composition comprising the vector.
摘要翻译： 通过将细胞周期蛋白和细胞周期蛋白依赖性激酶引入末端分化细胞的核中，然后培养或保持细胞，使末端分化的细胞增殖。 一种增殖终末分化细胞的方法，包括将编码核定位信号的核苷酸序列添加到细胞周期蛋白基因和细胞周期蛋白依赖性激酶基因中的至少一种，并将各基因体外引入末端分化细胞，然后培养 提供细胞，或者将每个基因直接引入体内终末分化的细胞。 细胞周期蛋白是可激活CDK4或CDK6的细胞周期蛋白，细胞周期蛋白依赖性激酶是由D型细胞周期蛋白激活的细胞周期蛋白依赖性激酶。 本发明还提供了用于这种方法的重组载体或包含该载体的药物组合物。
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2.发明授权公开(公告)号：US07795032B2
公开(公告)日：2010-09-14
申请号：US10713008
申请日：2003-11-17
申请人： Masaaki Ikeda , Mimi Adachi
发明人： Masaaki Ikeda , Mimi Adachi
CPC分类号： C12N5/0657 , A61K38/00 , C07K14/4738 , C12N2510/00 , C12N2799/022 , C12N2799/06
摘要： Terminal differentiated cells are proliferated by introducing a cyclin and a cyclin dependent kinase into the nucleus of terminal differentiated cells, and then cultivating or holding the cells. A method for proliferating terminal differentiated cells comprising adding a nucleotide sequence coding for a nuclear localization signal to at least one of a cyclin gene and a cyclin dependent kinase gene, and introducing each of the genes to terminal differentiated cells in vitro, and then cultivating the cells, or introducing each of the genes directly to terminal differentiated cells in vivo is provided. The cyclin is a cyclin that can activate CDK4 or CDK6, and the cyclin dependent kinase is a cyclin dependent kinase that is activated by D-type cyclin. The invention also provides a recombinant vector used for such a method or a pharmaceutical composition comprising the vector.
摘要翻译： 通过将细胞周期蛋白和细胞周期蛋白依赖性激酶引入末端分化细胞的核中，然后培养或保持细胞，使末端分化的细胞增殖。 一种增殖终末分化细胞的方法，包括将编码核定位信号的核苷酸序列添加到细胞周期蛋白基因和细胞周期蛋白依赖性激酶基因中的至少一种，并将各基因体外引入末端分化细胞，然后培养 提供细胞，或者将每个基因直接引入体内终末分化的细胞。 细胞周期蛋白是可激活CDK4或CDK6的细胞周期蛋白，细胞周期蛋白依赖性激酶是由D型细胞周期蛋白激活的细胞周期蛋白依赖性激酶。 本发明还提供了用于这种方法的重组载体或包含该载体的药物组合物。
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公开(公告)号：US20070009496A1
公开(公告)日：2007-01-11
申请号：US10580248
申请日：2004-11-19
CPC分类号： C12N5/0657 , A61K31/7088 , A61K35/12 , A61K48/00 , C07K14/4738 , C12N9/1205 , C12N2510/00
摘要： The proliferation of cardiomyocytes is induced by expressing cyclin and CDK in the cardiomyocytes, and by suppressing the function or action of a Cip/Kip family protein or inhibiting the production of a Cip/Kip family protein. Among the Cip/Kip family proteins, it is preferable to suppress the function of p27KiP1 or inhibiting the production thereof. As a recombinant vector to be used therefor, there is provided a vector comprising: (1) a cyclin gene; (2) a cyclin-dependent kinase gene; and (3) one or a plurality selected from the group consisting of a gene encoding a factor that inhibits the function or action of a Cip/Kip family protein and a nucleic acid sequence that inhibits the production of Cip/Kip family protein.
摘要翻译： 通过在心肌细胞中表达细胞周期蛋白和CDK，并通过抑制Cip / Kip家族蛋白的功能或作用或抑制Cip / Kip家族蛋白的产生来诱导心肌细胞的增殖。 在Cip / Kip家族蛋白质中，优选抑制p27 KiP1的功能或抑制其生产。 作为用于其的重组载体，提供了载体，其包含：（1）细胞周期蛋白基因; （2）细胞周期蛋白依赖性激酶基因; 和（3）选自由编码抑制Cip / Kip家族蛋白的功能或作用的因子的基因和抑制Cip / Kip家族蛋白的产生的核酸序列组成的组中的一种或多种。
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