公开(公告)号：US07358063B2

公开(公告)日：2008-04-15

申请号：US10484152

申请日：2002-07-17

申请人： Masaya Tohyama ,  Yuichi Yasuda ,  Yasushi Hasui ,  Hideki Ishihara ,  Koichi Yamagata

发明人： Masaya Tohyama ,  Yuichi Yasuda ,  Yasushi Hasui ,  Hideki Ishihara ,  Koichi Yamagata

IPC分类号： G01N33/53 ,  G01N33/537 ,  G01N33/542 ,  G01N1/30 ,  G01N33/566 ,  G01N33/567 ,  G01N33/48

CPC分类号： G01N33/6896

摘要： A method of detecting PS2V characterized by comprising reacting PS2V in a sample with a primary antibody which is bonded to a solid phase and specifically recognizes PA2V, then reacting with a secondary antibody recognizing PS2 or PS2V by any of the following procedures: (a) reacting with a secondary antibody having been enzyme-labeled and recognizing PS2 or PS2V; (b) reacting with a secondary antibody having been biotinylated and recognizing PS2 or PS2V and then reacting with an avidinylated or streptoavidinylated enzyme; (c) reacting with a secondary antibody having been biotinylated and recognizing PS2 or PS2V and then reacting with a biotinylated enzyme and avidin or streptoavidin; and (d) reacting with a secondary antibody recognizing PS2 or PS2V and then reacting with an antibody having been enzyme-labeled and recognizing the secondary antibody; then adding the substrate of the above enzyme and detecting the product formed by the enzyme reaction.

摘要翻译： 一种检测PS2V的方法，其特征在于包括使样品中的PS2V与与固相结合的一抗并特异性识别PA2V，然后通过以下任一方法与识别PS2或PS2V的二抗反应：（a）使 二抗被酶标记并识别PS2或PS2V; （b）与已经生物素化并识别PS2或PS2V的第二抗体反应，然后与二脱水或链脲霉素化酶反应; （c）与已经生物素化并识别PS2或PS2V的二级抗体反应，然后与生物素化的酶和抗生物素蛋白或链霉亲和素反应; 和（d）与识别PS2或PS2V的二抗反应，然后与经酶标记的抗体和识别第二抗体反应; 然后加入上述酶的底物并检测通过酶反应形成的产物。

公开(公告)号：US20050003339A1

公开(公告)日：2005-01-06

申请号：US10484152

申请日：2002-07-17

申请人： Masaya Tohyama ,  Yuichi Yasuda ,  Yasushi Hasui ,  Hideki Ishihara ,  Koichi Yamagata

发明人： Masaya Tohyama ,  Yuichi Yasuda ,  Yasushi Hasui ,  Hideki Ishihara ,  Koichi Yamagata

IPC分类号： G01N33/68 ,  C12Q1/70

CPC分类号： G01N33/6896

摘要： A method of detecting PS2V characterized by comprising reacting PS2V in a sample with a primary antibody which is bonded to a solid phase and specifically recognizes PA2V, then reacting with a secondary antibody recognizing PS2 or PS2V by any of the following procedures: (a) reacting with a secondary antibody having been enzyme-labeled and recognizing PS2 or PS2V; (b) reacting with a secondary antibody having been biotinylated and recognizing PS2 or PS2V and then reacting with an avidinylated or streptoavidinylated enzyme; (c) reacting with a secondary antibody having been biotinylated and recognizing PS2 or PS2V and then reacting with a biotinylated enzyme and avidin or streptoavidin; and (d) reacting with a secondary antibody recognizing PS2 or PS2V and then reacting with an antibody having been enzyme-labeled and recognizing the secondary antibody; then adding the substrate of the above enzyme and detecting the product formed by the enzyme reaction.

摘要翻译： 一种检测PS2V的方法，其特征在于包括使样品中的PS2V与与固相结合的一抗并特异性识别PA2V，然后通过以下任一方法与识别PS2或PS2V的二抗反应：（a）使 二抗被酶标记并识别PS2或PS2V; （b）与已经生物素化并识别PS2或PS2V的第二抗体反应，然后与二脱水或链脲霉素化酶反应; （c）与已经生物素化并识别PS2或PS2V的二级抗体反应，然后与生物素化的酶和抗生物素蛋白或链霉亲和素反应; 和（d）与识别PS2或PS2V的二抗反应，然后与经酶标记的抗体和识别第二抗体反应; 然后加入上述酶的底物并检测通过酶反应形成的产物。

公开(公告)号：US20060234253A1

公开(公告)日：2006-10-19

申请号：US11244045

申请日：2005-10-06

申请人： Yasushi Hasui ,  Shigeki Abe ,  Motonari Daito ,  Koichi Yamagata

发明人： Yasushi Hasui ,  Shigeki Abe ,  Motonari Daito ,  Koichi Yamagata

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6834 ,  C12Q2565/519 ,  C12Q2537/1373 ,  C12Q2537/125 ,  C12Q2537/1376

摘要： A method for detecting a target substance is described, which comprises the steps of: forming a first complex comprising (a) a first nucleic acid probe immobilized on a first solid phase and having a single-strand portion, (b) a nucleic acid ligand which has a binding section as a base sequence hybridizable with the single-strand portion of the first nucleic acid probe and which has a target-binding substance capable of binding to the target substance, (c) the target substance, and (d) a labeling substance capable of binding to the target substance; separating, from the first complex, a second complex comprising the nucleic acid ligand, the target substance and the labeling substance; and detecting the target substance on the basis of the labeling substance in the second complex.

摘要翻译： 描述了用于检测目标物质的方法，其包括以下步骤：形成第一配合物，其包含（a）固定在第一固相上并具有单链部分的第一核酸探针，（b）核酸配体 其具有与第一核酸探针的单链部分杂交的碱基序列的结合部分，其具有能够结合目标物质的靶结合物质，（c）靶物质，和（d） 能够结合目标物质的标记物质; 从第一复合物中分离出包含核酸配体，靶物质和标记物质的第二复合物; 并基于第二配合物中的标记物质检测目标物质。

公开(公告)号：US09102934B2

公开(公告)日：2015-08-11

申请号：US12057014

申请日：2008-03-27

申请人： Masatoshi Yamasaki ,  Yasushi Hasui ,  Chinatsu Fukui

发明人： Masatoshi Yamasaki ,  Yasushi Hasui ,  Chinatsu Fukui

IPC分类号： C12N15/85 ,  C12N15/10

CPC分类号： C12N15/1003

摘要： The present invention provides a pseudo-tissue for accuracy control comprising a nucleic acid or cells, a holding body for holding the nucleic acid or cells, and a protecting body for covering at least a part of the surface of the holding body so as to protect the holding body. A method for controlling accuracy by using the pseudo-tissue, and a method for manufacturing the pseudo-tissue are also disclosed.

摘要翻译： 本发明提供了一种用于精确控制的伪组织，其包括核酸或细胞，用于保持核酸或细胞的保持体，以及用于覆盖保持体的至少一部分表面的保护体，以保护 握持体。 还公开了通过使用伪组织来控制精度的方法和用于制造假组织的方法。

公开(公告)号：US07445135B2

公开(公告)日：2008-11-04

申请号：US10496124

申请日：2002-11-21

申请人： Taneo Miyata ,  Yasushi Hasui ,  Mikio Murakami ,  Hirofumi Fujiwara

发明人： Taneo Miyata ,  Yasushi Hasui ,  Mikio Murakami ,  Hirofumi Fujiwara

IPC分类号： B65D88/54 ,  B65D35/56

CPC分类号： B05C17/00513 ,  B05C17/00516 ,  B05C17/00583 ,  B05C17/01 ,  B65D83/0005

摘要： A container, which houses a liquid such as a sealant in a flexible pouch 11 in a sealed state, is provided with a spout 2 that is constituted by an extrusion nozzle 22 and a spout base member 21 and formed on one end thereof so that the extrusion nozzle 22 is fixed by sandwiching the vicinity of a hole 32a formed on one end face of an extruder 3 by a flange portion 21c of the spout base member 21 and a flange portion 22c of the extrusion nozzle 22, and an extruder 3a is arranged so that an adjusting member 322b, which controls the tilt of the nozzle 22, is allowed to protrude outward from a hole 322a of the extruder 3a.

摘要翻译： 在密封状态下在柔性袋11中容纳诸如密封剂的液体的容器设置有由挤出喷嘴22和喷口基部构件21构成并形成在其一端上的喷口2， 通过将喷口基体21的凸缘部21c和挤出喷嘴22的凸缘部22c夹在形成在挤出机3的一个端面上的孔32a附近，挤出喷嘴22被固定， 另外，如图3（a）所示，使得能够控制喷嘴22倾斜的调节构件322b从挤出机3a的孔322a向外突出。

公开(公告)号：US20090053711A1

公开(公告)日：2009-02-26

申请号：US12057014

申请日：2008-03-27

申请人： Masatoshi Yamasaki ,  Yasushi Hasui ,  Chinatsu Fukui

发明人： Masatoshi Yamasaki ,  Yasushi Hasui ,  Chinatsu Fukui

IPC分类号： C12Q1/68

CPC分类号： C12N15/1003

摘要： The present invention provides a pseudo-tissue for accuracy control comprising a nucleic acid or cells, a holding body for holding the nucleic acid or cells, and a protecting body for covering at least a part of the surface of the holding body so as to protect the holding body. A method for controlling accuracy by using the pseudo-tissue, and a method for manufacturing the pseudo-tissue are also disclosed.

摘要翻译： 本发明提供了一种用于精确控制的伪组织，其包括核酸或细胞，用于保持核酸或细胞的保持体，以及用于覆盖保持体的至少一部分表面的保护体，以保护 握持体。 还公开了通过使用伪组织来控制精度的方法和用于制造假组织的方法。

公开(公告)号：US20050006413A1

公开(公告)日：2005-01-13

申请号：US10496124

申请日：2002-11-21

申请人： Taneo Miyata ,  Yasushi Hasui ,  Mikio Murakami ,  Hirofumi Fujiwara

发明人： Taneo Miyata ,  Yasushi Hasui ,  Mikio Murakami ,  Hirofumi Fujiwara

IPC分类号： B05C17/005 ,  B05C17/01 ,  B65D33/36 ,  B65D35/30 ,  B65D83/00 ,  B65D35/28 ,  B65D35/56

CPC分类号： B05C17/00513 ,  B05C17/00516 ,  B05C17/00583 ,  B05C17/01 ,  B65D83/0005

摘要： A container, which houses a liquid such as a sealant in a flexible pouch 11 in a sealed state, is provided with a spout 2 that is constituted by an extrusion nozzle 22 and a spout base member 21 and formed on one end thereof so that the extrusion nozzle 22 is fixed by sandwiching the vicinity of a hole 32a formed on one end face of an extruder 3 by a flange portion 21c of the spout base member 21 and a flange portion 22c of the extrusion nozzle 22, and an extruder 3a is arranged so that an adjusting member 322b, which controls the tilt of the nozzle 22, is allowed to protrude outward from a hole 322a of the extruder 3a.

摘要翻译： 在密封状态下在柔性袋11中容纳诸如密封剂的液体的容器设置有由挤出喷嘴22和喷口基部构件21构成并形成在其一端上的喷口2， 通过将喷口基体21的凸缘部21c和挤出喷嘴22的凸缘部22c夹在形成在挤出机3的一个端面上的孔32a的附近，挤出喷嘴22被固定，挤出机3a 使得控制喷嘴22的倾斜的调节构件322b允许从挤出机3a的孔322a向外突出。