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    Method for detecting a target substance by using a nucleic acid probe
    1.
    发明申请
    Method for detecting a target substance by using a nucleic acid probe 审中-公开
    通过使用核酸探针检测目标物质的方法

    公开(公告)号：US20060234253A1

    公开(公告)日：2006-10-19

    申请号：US11244045

    申请日：2005-10-06

    申请人： Yasushi Hasui ,  Shigeki Abe ,  Motonari Daito ,  Koichi Yamagata

    发明人： Yasushi Hasui ,  Shigeki Abe ,  Motonari Daito ,  Koichi Yamagata

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6834 ,  C12Q2565/519 ,  C12Q2537/1373 ,  C12Q2537/125 ,  C12Q2537/1376

    摘要： A method for detecting a target substance is described, which comprises the steps of: forming a first complex comprising (a) a first nucleic acid probe immobilized on a first solid phase and having a single-strand portion, (b) a nucleic acid ligand which has a binding section as a base sequence hybridizable with the single-strand portion of the first nucleic acid probe and which has a target-binding substance capable of binding to the target substance, (c) the target substance, and (d) a labeling substance capable of binding to the target substance; separating, from the first complex, a second complex comprising the nucleic acid ligand, the target substance and the labeling substance; and detecting the target substance on the basis of the labeling substance in the second complex.

    摘要翻译： 描述了用于检测目标物质的方法，其包括以下步骤：形成第一配合物，其包含（a）固定在第一固相上并具有单链部分的第一核酸探针，（b）核酸配体 其具有与第一核酸探针的单链部分杂交的碱基序列的结合部分，其具有能够结合目标物质的靶结合物质，（c）靶物质，和（d） 能够结合目标物质的标记物质; 从第一复合物中分离出包含核酸配体，靶物质和标记物质的第二复合物; 并基于第二配合物中的标记物质检测目标物质。

    Probe set for detection of target substance and detection method using the same
    2.
    发明申请
    Probe set for detection of target substance and detection method using the same 失效
    用于检测目标物质的探针和使用其的检测方法

    公开(公告)号：US20050208556A1

    公开(公告)日：2005-09-22

    申请号：US11083951

    申请日：2005-03-21

    申请人： Motonari Daito ,  Koichi Yamagata ,  Yasuyuki Imura

    发明人： Motonari Daito ,  Koichi Yamagata ,  Yasuyuki Imura

    IPC分类号： C07H21/04 ,  C12Q1/68

    CPC分类号： C12Q1/6813 ,  C12Q2533/107 ,  C12Q2525/161

    摘要： A probe set is described that includes a first probe having a target-binding substance and a base sequence X1, a second probe having a base sequence X2c and a base sequence X1c hybridizable with the base sequence X1, and a third probe having a base sequence X2 hybridizable with the base sequence X2c. A method for detecting a target substance is also described.

    摘要翻译： 描述了一种探针组，其包括具有靶结合物质和碱基序列X1的第一探针，具有碱基序列X2c的第二探针和与碱基序列X1可杂交的碱基序列X1c，以及具有碱基序列的第三探针 X2可与碱基序列X2c杂交。 还描述了用于检测目标物质的方法。

    Method of detecting PS2V
    4.
    发明授权
    Method of detecting PS2V 有权
    检测PS2V的方法

    公开(公告)号：US07358063B2

    公开(公告)日：2008-04-15

    申请号：US10484152

    申请日：2002-07-17

    申请人： Masaya Tohyama ,  Yuichi Yasuda ,  Yasushi Hasui ,  Hideki Ishihara ,  Koichi Yamagata

    发明人： Masaya Tohyama ,  Yuichi Yasuda ,  Yasushi Hasui ,  Hideki Ishihara ,  Koichi Yamagata

    IPC分类号： G01N33/53 ,  G01N33/537 ,  G01N33/542 ,  G01N1/30 ,  G01N33/566 ,  G01N33/567 ,  G01N33/48

    CPC分类号： G01N33/6896

    摘要： A method of detecting PS2V characterized by comprising reacting PS2V in a sample with a primary antibody which is bonded to a solid phase and specifically recognizes PA2V, then reacting with a secondary antibody recognizing PS2 or PS2V by any of the following procedures: (a) reacting with a secondary antibody having been enzyme-labeled and recognizing PS2 or PS2V; (b) reacting with a secondary antibody having been biotinylated and recognizing PS2 or PS2V and then reacting with an avidinylated or streptoavidinylated enzyme; (c) reacting with a secondary antibody having been biotinylated and recognizing PS2 or PS2V and then reacting with a biotinylated enzyme and avidin or streptoavidin; and (d) reacting with a secondary antibody recognizing PS2 or PS2V and then reacting with an antibody having been enzyme-labeled and recognizing the secondary antibody; then adding the substrate of the above enzyme and detecting the product formed by the enzyme reaction.

    摘要翻译： 一种检测PS2V的方法，其特征在于包括使样品中的PS2V与与固相结合的一抗并特异性识别PA2V，然后通过以下任一方法与识别PS2或PS2V的二抗反应：（a）使 二抗被酶标记并识别PS2或PS2V; （b）与已经生物素化并识别PS2或PS2V的第二抗体反应，然后与二脱水或链脲霉素化酶反应; （c）与已经生物素化并识别PS2或PS2V的二级抗体反应，然后与生物素化的酶和抗生物素蛋白或链霉亲和素反应; 和（d）与识别PS2或PS2V的二抗反应，然后与经酶标记的抗体和识别第二抗体反应; 然后加入上述酶的底物并检测通过酶反应形成的产物。

    Method of detecting ps2v
    5.
    发明申请
    Method of detecting ps2v 有权
    检测ps2v的方法

    公开(公告)号：US20050003339A1

    公开(公告)日：2005-01-06

    申请号：US10484152

    申请日：2002-07-17

    申请人： Masaya Tohyama ,  Yuichi Yasuda ,  Yasushi Hasui ,  Hideki Ishihara ,  Koichi Yamagata

    发明人： Masaya Tohyama ,  Yuichi Yasuda ,  Yasushi Hasui ,  Hideki Ishihara ,  Koichi Yamagata

    IPC分类号： G01N33/68 ,  C12Q1/70

    CPC分类号： G01N33/6896

    摘要： A method of detecting PS2V characterized by comprising reacting PS2V in a sample with a primary antibody which is bonded to a solid phase and specifically recognizes PA2V, then reacting with a secondary antibody recognizing PS2 or PS2V by any of the following procedures: (a) reacting with a secondary antibody having been enzyme-labeled and recognizing PS2 or PS2V; (b) reacting with a secondary antibody having been biotinylated and recognizing PS2 or PS2V and then reacting with an avidinylated or streptoavidinylated enzyme; (c) reacting with a secondary antibody having been biotinylated and recognizing PS2 or PS2V and then reacting with a biotinylated enzyme and avidin or streptoavidin; and (d) reacting with a secondary antibody recognizing PS2 or PS2V and then reacting with an antibody having been enzyme-labeled and recognizing the secondary antibody; then adding the substrate of the above enzyme and detecting the product formed by the enzyme reaction.

    摘要翻译： 一种检测PS2V的方法，其特征在于包括使样品中的PS2V与与固相结合的一抗并特异性识别PA2V，然后通过以下任一方法与识别PS2或PS2V的二抗反应：（a）使 二抗被酶标记并识别PS2或PS2V; （b）与已经生物素化并识别PS2或PS2V的第二抗体反应，然后与二脱水或链脲霉素化酶反应; （c）与已经生物素化并识别PS2或PS2V的二级抗体反应，然后与生物素化的酶和抗生物素蛋白或链霉亲和素反应; 和（d）与识别PS2或PS2V的二抗反应，然后与经酶标记的抗体和识别第二抗体反应; 然后加入上述酶的底物并检测通过酶反应形成的产物。

    METHOD AND KIT FOR DETERMINING THE AMOUNT OF DNA BINDING PROTEIN
    6.
    发明申请
    METHOD AND KIT FOR DETERMINING THE AMOUNT OF DNA BINDING PROTEIN 审中-公开
    用于测定DNA结合蛋白质量的方法和试剂盒

    公开(公告)号：US20090004749A1

    公开(公告)日：2009-01-01

    申请号：US12163431

    申请日：2008-06-27

    申请人： Koichi Yamagata ,  Shigeki Abe

    发明人： Koichi Yamagata ,  Shigeki Abe

    IPC分类号： G01N33/00

    CPC分类号： G01N33/5308 ,  G01N33/6872

    摘要： A kit for determining a quantity of a target DNA binding protein in a liquid sample by using a fluorescence correlation spectroscopy, comprising a first measuring reagent including a fluorescent-labeled nucleic acid probe and a first unlabeled nucleic acid probe, a second measuring reagent including the fluorescent-labeled nucleic acid probe and a second unlabeled nucleic acid probe, wherein the target DNA binding protein is capable of binding to the fluorescent-labeled nucleic acid probe and the first unlabeled nucleic acid probe, and is not capable of binding to the second unlabeled nucleic acid probe; and a method for determining a quantity of a target DNA binding protein in a liquid sample is also disclosed.

    摘要翻译： 一种用于通过使用荧光相关光谱测定液体样品中的靶DNA结合蛋白的量的试剂盒，其包括含有荧光标记的核酸探针和第一未标记的核酸探针的第一测定试剂，包含第 荧光标记的核酸探针和第二个未标记的核酸探针，其中靶DNA结合蛋白能够结合荧光标记的核酸探针和第一个未标记的核酸探针，并且不能结合第二个未标记的核酸探针 核酸探针; 还公开了一种用于测定液体样品中目标DNA结合蛋白的量的方法。

    Tissue characteristic determination apparatus
    8.
    发明授权
    Tissue characteristic determination apparatus 有权
    组织特征确定装置

    公开(公告)号：US07700346B2

    公开(公告)日：2010-04-20

    申请号：US11520984

    申请日：2006-09-14

    申请人： Hironori Kobayashi ,  Hideki Ishihara ,  Koichi Yamagata

    发明人： Hironori Kobayashi ,  Hideki Ishihara ,  Koichi Yamagata

    IPC分类号： C12M1/40 ,  G01N33/00 ,  C12Q1/00 ,  G01N33/574

    CPC分类号： G01N33/5011 ,  G01N33/5302 ,  G01N2333/912 ,  G01N2800/52

    摘要： The tissue characteristic determination apparatus capable of determining the characteristics of the tissues collected from a living organism is described, a representative one of which includes: a first data obtainer for obtaining first data reflecting the activity of first cyclin-dependent kinase contained in samples prepared from the tissues, a second data obtainer for obtaining second data reflecting the expression level of the first cyclin-dependent kinase, and a tissue characteristics information obtainer for obtaining information on the characteristics of the tissues based on first values obtained from the first and second data.

    摘要翻译： 描述了能够确定从活体收集的组织的特征的组织特征确定装置，其代表性的方法包括：第一数据获取器，用于获得反映由第一细胞周期蛋白依赖激酶 组织，用于获得反映第一细胞周期蛋白依赖性激酶的表达水平的第二数据的第二数据获取器，以及用于基于从第一和第二数据获得的第一值获得关于组织特征的信息的组织特征信息获取器。

    Nucleic acid detection method and system thereof
    9.
    发明申请
    Nucleic acid detection method and system thereof 有权
    核酸检测方法及其系统

    公开(公告)号：US20050089857A1

    公开(公告)日：2005-04-28

    申请号：US10491981

    申请日：2003-01-08

    申请人： Sachiyo Tada ,  Tomokazu Yoshida ,  Keiichiro Shohmi ,  Masahiro Nishida ,  Kazuki Nakabayashi ,  Koichi Yamagata

    发明人： Sachiyo Tada ,  Tomokazu Yoshida ,  Keiichiro Shohmi ,  Masahiro Nishida ,  Kazuki Nakabayashi ,  Koichi Yamagata

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6806 ,  C12Q1/6844 ,  C12Q2531/113 ,  C12Q2527/127 ,  C12Q2525/119

    摘要： In detecting a nucleic acid by using the nucleic acid amplification method, it is intended to shorten the time required for the measurement so as to systematically and efficiently examine a gene, etc. To achieve this object, use is made of a method of directly amplifying a nucleic acid by treating a collected biological sample wherein a means of inhibiting the degradation activity of the nucleic acid is introduced during or after the step of homogenizing the biological sample and thus the nucleic acid is directly amplified without isolating/purifying the nucleic acid component from the biological sample. In this method, the means of inhibiting the degradation activity of the nucleic acid is introduced at an acidic pH value (more specifically, from pH 2.5 to pH 5) with the use of a salt interacting with a substance inhibiting the nucleic acid amplification reaction and/or the nucleic acid component so as to directly amplify the nucleic acid without isolating/purifying the nucleic acid component.

    摘要翻译： 通过使用核酸扩增法检测核酸，旨在缩短测定所需的时间，从而系统地且有效地检查基因等。为了达到这个目的，使用直接扩增方法 通过处理收集的生物样品的核酸，其中在均化生物样品的步骤期间或之后引入抑制核酸降解活性的手段，因此核酸直接扩增而不从核酸组分分离/纯化 生物样品。 在该方法中，使用与抑制核酸扩增反应的物质相互作用的盐，在酸性pH值（更具体地，pH 2.5〜pH 5））下引入抑制核酸降解活性的方法，以及 /或核酸组分，以便直接扩增核酸而不分离/纯化核酸组分。

    Oligonucleotides for detecting bacteria and detection method using same
    10.
    发明授权
    Oligonucleotides for detecting bacteria and detection method using same 失效
    用于检测细菌的寡核苷酸及其检测方法

    公开(公告)号：US5525718A

    公开(公告)日：1996-06-11

    申请号：US379296

    申请日：1995-01-27

    申请人： Tetsuo Ohashi ,  Jun Tada ,  Shigeru Fukushima ,  Hiroko Ozaki ,  Naoyuki Nishimura ,  Yoshinari Shirasaki ,  Koichi Yamagata

    发明人： Tetsuo Ohashi ,  Jun Tada ,  Shigeru Fukushima ,  Hiroko Ozaki ,  Naoyuki Nishimura ,  Yoshinari Shirasaki ,  Koichi Yamagata

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12N1/00 ,  C12N15/00

    CPC分类号： C12Q1/689 ,  Y10S435/848 ,  Y10S435/909

    摘要： Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.

    摘要翻译： 可以与副溶血弧菌的特异性基因选择性杂交的寡核苷酸（SEQ ID NO：1-8），可选择性地与产生性埃希氏弧线圈的LT基因杂交的寡核苷酸（SEQ ID NO：9-13），选择性可混合的寡核苷酸（SEQ ID NO：14-21） 与产毒性埃希氏胆碱圈的STh或STp基因，选择性地与金黄色葡萄球菌的entA，B，C或D基因杂交的寡核苷酸（SEQ ID NO：22-47）或选择性地与金黄色葡萄球菌选择性杂交的寡核苷酸（SEQ ID NO：48-53） 制备Staplyloccus金黄色葡萄球菌的entE基因并用作基因扩增的引物，从而仅选择性地检测引起食物中毒的相应微生物。