公开(公告)号：US20210310022A1

公开(公告)日：2021-10-07

申请号：US17338027

申请日：2021-06-03

Applicant: Massachusetts Institute of Technology

Inventor： Timothy Kuan-Ta Lu ,  Alan Siu Lun Wong ,  Ching Gee Choi

IPC: C12N15/85 ,  C12N15/10 ,  A61K31/4709 ,  A61K31/55

Abstract: Described herein are methods and compositions that enable rapid generation of high-order combinations of genetic elements comprising a CRISPR guide sequence and a scaffold sequence, and a barcode for rapid identification of the combination of genetic elements encoded within a single cell or a pooled population. Also described herein compositions of inhibitors of epigenetic genes and methods for reducing cell proliferation and/or treating cancer.

公开(公告)号：US20190100769A1

公开(公告)日：2019-04-04

申请号：US15521931

申请日：2015-10-30

Applicant: Massachusetts Institute of Technology

Inventor： Timothy Kuan-Ta Lu ,  Alan Siu Lun Wong ,  Ching Gee Choi

IPC: C12N15/85 ,  C12N15/10 ,  A61K31/55 ,  A61K31/4709

Abstract: Described herein are methods and compositions that enable rapid generation of high-order combinations of genetic elements comprising a CRISPR guide sequence and a scaffold sequence, and a barcode for rapid identification of the combination of genetic elements encoded within a single cell or a pooled population. Also described herein compositions of inhibitors of epigenetic genes and methods for reducing cell proliferation and/or treating cancer.

公开(公告)号：US20170369878A1

公开(公告)日：2017-12-28

申请号：US15542670

申请日：2016-01-11

Applicant: Massachusetts Institute of Technology

Inventor： Timothy Kuan-Ta Lu ,  Alan Siu Lun Wong ,  Ching Gee Choi

IPC: C12N15/113 ,  A61K31/7088 ,  A61K31/337 ,  G01N33/50 ,  A61K45/06

CPC classification number: C12N15/113 ,  A61K31/337 ,  A61K31/7088 ,  A61K45/06 ,  C12N15/111 ,  C12N2310/141 ,  C12N2310/51 ,  G01N33/5011

Abstract: Described herein are methods and compositions of combinations of microRNAs that enhance the sensitivity of cancer cells to chemotherapeutic agents or reduce proliferation of cancer cells. Also described herein are methods for the identification of combinations of microRNAs that result in desired effects.