公开(公告)号：US20120107364A1

公开(公告)日：2012-05-03

申请号：US13382468

申请日：2010-07-07

申请人： Masunori Kajikawa ,  Masahito Sugiura ,  Kazuyuki Atarashi ,  Emi Shimizu ,  Chiemi Matsumi ,  Yukie Saitoh

发明人： Masunori Kajikawa ,  Masahito Sugiura ,  Kazuyuki Atarashi ,  Emi Shimizu ,  Chiemi Matsumi ,  Yukie Saitoh

IPC分类号： A61K39/00 ,  A61P37/04 ,  C12N5/16 ,  A61P35/00 ,  C07K16/30 ,  C12N15/13

CPC分类号： C07K16/30 ,  A61K2039/505 ,  C07K16/3046 ,  C07K2317/73

摘要： By utilizing an SST-REX method, a cDNA encoding a protein expressed on a cell surface or secreted from the cell was selected from a cDNA library derived from a cancer cell line. Monoclonal antibodies against the protein encoding the selected cDNA were prepared. The in vitro and in vivo anti-cancer activities and binding to various cancer cells lines were examined. As a result, a monoclonal antibody which binds to a PODXL2 protein, and which had excellent anti-cancer activities was found. Further, a region including an epitope of the antibody was successfully identified, and the amino acid sequences of variable regions of a light chain and a heavy chain were successfully determined.

摘要翻译： 通过使用SST-REX方法，从源自癌细胞系的cDNA文库中选择编码在细胞表面表达或从细胞分泌的蛋白质的cDNA。 制备针对编码所选cDNA的蛋白质的单克隆抗体。 检查体外和体内抗癌活性并结合各种癌细胞系。 结果发现结合PODXL2蛋白的单克隆抗体具有优异的抗癌活性。 此外，成功鉴定了包含抗体的表位的区域，成功地测定了轻链和重链的可变区的氨基酸序列。

公开(公告)号：US08828387B2

公开(公告)日：2014-09-09

申请号：US13382468

申请日：2010-07-07

申请人： Masunori Kajikawa ,  Masahito Sugiura ,  Kazuyuki Atarashi ,  Emi Shimizu ,  Chiemi Matsumi ,  Yukie Saitoh

发明人： Masunori Kajikawa ,  Masahito Sugiura ,  Kazuyuki Atarashi ,  Emi Shimizu ,  Chiemi Matsumi ,  Yukie Saitoh

IPC分类号： A61K39/395 ,  C07K16/30 ,  A61K39/00

CPC分类号： C07K16/30 ,  A61K2039/505 ,  C07K16/3046 ,  C07K2317/73

摘要： By utilizing an SST-REX method, a cDNA encoding a protein expressed on a cell surface or secreted from the cell was selected from a cDNA library derived from a cancer cell line. Monoclonal antibodies against the protein encoding the selected cDNA were prepared. The in vitro and in vivo anti-cancer activities and binding to various cancer cells lines were examined. As a result, a monoclonal antibody which binds to a PODXL2 protein, and which had excellent anti-cancer activities was found. Further, a region including an epitope of the antibody was successfully identified, and the amino acid sequences of variable regions of a light chain and a heavy chain were successfully determined.

摘要翻译： 通过使用SST-REX方法，从源自癌细胞系的cDNA文库中选择编码在细胞表面表达或从细胞分泌的蛋白质的cDNA。 制备针对编码所选cDNA的蛋白质的单克隆抗体。 检查体外和体内抗癌活性并结合各种癌细胞系。 结果发现结合PODXL2蛋白的单克隆抗体具有优异的抗癌活性。 此外，成功鉴定了包含抗体的表位的区域，成功地测定了轻链和重链的可变区的氨基酸序列。