公开(公告)号：US07488588B1

公开(公告)日：2009-02-10

申请号：US10203639

申请日：2000-11-14

申请人： Matti Airaksinen ,  Mart Saarma ,  Dimitri Poteriaev ,  Maria Lindahl ,  Tönis Timmusk ,  Jari Rossi

发明人： Matti Airaksinen ,  Mart Saarma ,  Dimitri Poteriaev ,  Maria Lindahl ,  Tönis Timmusk ,  Jari Rossi

IPC分类号： C07H21/02 ,  C07H21/04 ,  C07K1/00 ,  C12P21/06 ,  C12N5/00 ,  C12N15/00

CPC分类号： C07K14/71 ,  A61K38/00 ,  A61K48/00

摘要： The present invention discloses purified and isolated nucleic acid sequences encoding polypeptides having a structure substantially similar to that of splicing forms of mammalian GFRα4 comprising the amino acid sequence (SEQ ID NO:1:)-(SEQ ID NO:6:). The preferred sequences comprise cDNAs having the sequence (SEQ ID NO:7:)-(SEQ ID NO:13:). The present invention is also related to purified and isolated polypeptides comprising the amino acid sequence and or substantially similar splicing forms of mammalian GFRà4. Furthermore, the invention is related to substances capable of specifically recognizing said polypeptides and including both antibodies and receptors. The active compounds of the present invention including cDNAS, polypeptides, binding substances, and antibodies. The invention is useful not only for producing cell-lines and/or transgenic non-human animal but also for diagnosing and treating neuronal disorders or endocrine tumors as well as other related diseases by enabling measurements of GFRα4-mediated signalling in endocrine cells and neurons.

摘要翻译： 本发明公开了编码具有与包含氨基酸序列（SEQ ID NO：1：） - （SEQ ID NO：6））的哺乳动物GFRα4剪接形式基本相似的结构的多肽的纯化和分离的核酸序列。 优选的序列包含具有序列（SEQ ID NO：7：） - （SEQ ID NO：13 :)的cDNA。 本发明还涉及包含哺乳动物GFRà4的氨基酸序列和/或基本相似的剪接形式的纯化和分离的多肽。 此外，本发明涉及能够特异性识别所述多​​肽并包括抗体和受体的物质。 本发明的活性化合物包括cDNAS，多肽，结合物质和抗体。 本发明不仅可用于生产细胞系和/或转基因非人类动物，还可用于诊断和治疗神经元疾病或内分泌肿瘤以及其他相关疾病，方法是测量GFRalpha4介导的内分泌细胞和神经元信号。