公开(公告)号：US20160264935A1

公开(公告)日：2016-09-15

申请号：US15164528

申请日：2016-05-25

Applicant: McMaster University

Inventor： Mickie Bhatia ,  Tamra Werbowetski-Ogilvie ,  Eleftherios Sachlos ,  Daniela Fischer Russell ,  Sarah Laronde ,  JungBok Lee ,  Eva Szabo ,  Ruth Risueno

IPC: C12N5/0735 ,  C12Q1/04 ,  G01N33/50

CPC classification number: C12N5/0606 ,  C12N5/0695 ,  C12N2501/115 ,  C12Q1/04 ,  G01N33/5073 ,  G01N2500/10

Abstract: The present disclosure provides transformed human pluripotent stem cell (t-hPSC). t-hPSCs are not dependent on Oct4 for renewal and survival, however exhibit a sensitivity to reduced levels of the transcription factor Nanog. Also provided are methods of culturing cells for use in a cell-based screening assay comprising placing one or more transformed human pluripotent stem cells into a receptacle and culturing said stem cells in the receptacle to form a monolayer of stem cells without cell overlap. Methods of screening compounds using t-hPSCs are also described.

公开(公告)号：US20150038371A1

公开(公告)日：2015-02-05

申请号：US14329412

申请日：2014-07-11

Applicant: McMaster University

Inventor： Mickie Bhatia ,  Tamra Werbowetski-Ogilvie ,  Eleftherios Sachlos ,  Daniela Fischer Russell ,  Sarah Laronde ,  JungBok Lee ,  Eva Szabo ,  Ruth Risueno

IPC: C12N5/0735 ,  C12Q1/68 ,  C12N5/095 ,  G01N33/50 ,  G01N33/569

CPC classification number: C12N5/0606 ,  C12N5/0695 ,  C12N2501/115 ,  C12Q1/04 ,  G01N33/5073 ,  G01N2500/10

Abstract: The present disclosure provides transformed human pluripotent stem cell (t-hPSC). t-hPSCs are not dependent on Oct4 for renewal and survival, however exhibit a sensitivity to reduced levels of the transcription factor Nanog. Also provided are methods of culturing cells for use in a cell-based screening assay comprising placing one or more transformed human pluripotent stem cells into a receptacle and culturing said stem cells in the receptacle to form a monolayer of stem cells without cell overlap. Methods of screening compounds using t-hPSCs are also described.

Abstract translation: 本公开提供了转化的人多能干细胞（t-hPSC）。 t-hPSC不依赖Oct4进行更新和生存，但对转录因子Nanog的水平降低表现出敏感性。 还提供培养用于基于细胞的筛选测定中的细胞的方法，包括将一个或多个转化的人多能干细胞置于容器中并在容器中培养所述干细胞以形成无细胞重叠的干细胞单层。 还描述了使用t-hPSC筛选化合物的方法。

公开(公告)号：US09365821B2

公开(公告)日：2016-06-14

申请号：US14329412

申请日：2014-07-11

Applicant: McMaster University

Inventor： Mickie Bhatia ,  Tamra Werbowetski-Ogilvie ,  Eleftherios Sachlos ,  Daniela Fischer Russell ,  Sarah Laronde ,  JungBok Lee ,  Eva Szabo ,  Ruth Risueno

IPC: C12N15/00 ,  C12N5/0735 ,  G01N33/50 ,  C12N5/095

CPC classification number: C12N5/0606 ,  C12N5/0695 ,  C12N2501/115 ,  C12Q1/04 ,  G01N33/5073 ,  G01N2500/10

Abstract: The present disclosure provides transformed human pluripotent stem cell (t-hPSC). t-hPSCs are not dependent on Oct4 for renewal and survival, however exhibit a sensitivity to reduced levels of the transcription factor Nanog. Also provided are methods of culturing cells for use in a cell-based screening assay comprising placing one or more transformed human pluripotent stem cells into a receptacle and culturing said stem cells in the receptacle to form a monolayer of stem cells without cell overlap. Methods of screening compounds using t-hPSCs are also described.

Abstract translation: 本公开提供了转化的人多能干细胞（t-hPSC）。 t-hPSC不依赖Oct4进行更新和生存，但对转录因子Nanog的降低水平表现出敏感性。 还提供培养用于基于细胞的筛选测定中的细胞的方法，包括将一个或多个转化的人多能干细胞置于容器中并在容器中培养所述干细胞以形成无细胞重叠的干细胞单层。 还描述了使用t-hPSC筛选化合物的方法。