公开(公告)号：US20240263163A1

公开(公告)日：2024-08-08

申请号：US18565957

申请日：2022-06-01

Applicant: McMaster University

Inventor： Tamaghna Gupta ,  Rakesh P. Sahu ,  Ishwar K. Puri

IPC: C12N13/00 ,  C12M1/32 ,  C12M1/42 ,  C12N5/071

CPC classification number: C12N13/00 ,  C12M23/12 ,  C12M35/06 ,  C12N5/0688 ,  C12N2529/00 ,  C12N2533/90

Abstract: This application relates to methods of generating cell/particle-free regions in a cellular/particle aggregate using label-free magnetic manipulation. For example, the method may include suspending cells/particles in a paramagnetic culture medium, the cells/particles being diamagnetic; seeding the cells/particles and the paramagnetic culture medium into microwells; and placing the microwells over an array of magnet pairs where each magnet pair creates a magnetic field gradient. The magnetic susceptibility difference and the magnetic field gradient drive the cells/particles towards a spaced region between magnets in the magnet pair having a lowest magnetic field strength to create a cell/particle aggregate with a cell-free/particle-free area where the cell/particle aggregate surrounds the cell-free/particle-free area.