公开(公告)号：US20240226255A9

公开(公告)日：2024-07-11

申请号：US18277897

申请日：2022-02-17

申请人： The Regents of the University of California

发明人： Anahid Jewett

IPC分类号： A61K39/00 ,  C12N5/09 ,  C12N15/86

CPC分类号： A61K39/0011 ,  C12N5/0693 ,  C12N15/86 ,  A61K2039/5152 ,  A61K2039/572 ,  C12N2529/00 ,  C12N2710/16143

摘要： The present invention is based, in part, on cancer vaccine compositions or pharmaceutical compositions comprising cancer cells, monocytes, and/or osteoclasts that activate NK cells, and methods for using same to prevent and/or treat diseases such as cancer.

公开(公告)号：US11980642B2

公开(公告)日：2024-05-14

申请号：US16470584

申请日：2017-11-01

申请人： DONGGUK UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

发明人： Jongpil Kim ,  Junsang Yoo

IPC分类号： A61K35/30 ,  C12N5/079 ,  C12N13/00

CPC分类号： A61K35/30 ,  C12N5/0618 ,  C12N13/00 ,  C12N2513/00 ,  C12N2529/00

摘要： The present invention relates to a method of inducing differentiation into a 3D dopaminergic midbrain organoid using a specific electromagnetic wave. It was specifically identified that the method makes it possible to remarkably improve production efficiency of a dopaminergic neuronal 3D-differentiated organoid, from which symptoms of Parkinson's disease can be effectively alleviated. Thus, it is anticipated that the present invention is capable of making a more fundamental approach and achieving targeted therapies in the treatment of a cranial nerve disease.

公开(公告)号：US11965178B2

公开(公告)日：2024-04-23

申请号：US16698645

申请日：2019-11-27

申请人： Cellphire, Inc.

发明人： Keith Andrew Moskowitz ,  Rafael Jorda ,  Ying Yi Zheng ,  Daniel Allen Sheik

IPC分类号： C12N5/078 ,  A01N1/02 ,  A61K9/127 ,  A61K9/50 ,  A61K31/337 ,  A61K31/502 ,  A61K31/704 ,  A61K35/19

CPC分类号： C12N5/0644 ,  A01N1/021 ,  A01N1/0221 ,  A01N1/0226 ,  A01N1/0284 ,  A61K9/1271 ,  A61K9/5068 ,  A61K31/337 ,  A61K31/502 ,  A61K31/704 ,  A61K35/19 ,  C12N2501/02 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2529/00

摘要： In some embodiments provided herein is a method of preparing cargo-loaded platelets, comprising: treating platelets with a cargo and with a loading buffer comprising a salt, a base, a loading agent, and optionally ethanol, to form the cargo-loaded platelets.

公开(公告)号：US20240110174A1

公开(公告)日：2024-04-04

申请号：US18477010

申请日：2023-09-28

申请人： Novocure GmbH

发明人： Tali VOLOSHIN-SELA ,  Yiftah BARSHESHET ,  Gadi COHEN ,  IIan VOLOVITZ ,  Gilad LEHMANN

IPC分类号： C12N13/00 ,  A61K39/00 ,  C12N5/0784

CPC分类号： C12N13/00 ,  A61K39/0011 ,  C12N5/0639 ,  A61K2039/5154 ,  C12N2501/052 ,  C12N2502/30 ,  C12N2529/00

摘要： Compositions, systems, and methods for activating dendritic cells are disclosed. Also disclosed are compositions, systems, and methods for reducing viability of cancer cells and treating cancer, as well as preventing an increase in volume of a tumor present in a body of a living subject, along with methods of treating other diseases and infections. The systems and methods involve application of an alternating electric field to dendritic cell(s) in vivo or ex vivo and/or to a subject or cancer cells isolated therefrom. The systems and methods may further include administration of activated dendritic cells to a subject. The compositions comprise populations of isolated dendritic cells activated by exposure to an alternating electric field.

公开(公告)号：US20230321148A1

公开(公告)日：2023-10-12

申请号：US18204999

申请日：2023-06-02

申请人： Maco Pharma

发明人： Bruno Delorme ,  Sabrina Viau ,  Francis Goudaliez

IPC分类号： A61K35/19 ,  A61L2/00 ,  C12N5/078 ,  C12N5/0775

CPC分类号： A61K35/19 ,  A61L2/0035 ,  C12N5/0644 ,  C12N5/0662 ,  C12N2500/84 ,  C12N2501/115 ,  C12N2529/00

摘要： A sterilised platelet lysate obtained with a method for sterilising a platelet lysate in the liquid state comprising at least the endogenous growth factors TGF-beta 1, EGF, PDGF-AB, IGF-1, VEGF and bFGF. The method comprising freezing the liquid platelet lysate in order to obtain a frozen platelet lysate, and irradiating the frozen platelet lysate with ionising radiation in order to obtain a sterilised platelet lysate, the irradiation being adapted so as to preserve at least 80% of the concentration of at least one of the endogenous growth factors chosen from the group consisting of TGF-beta 1, EGF, PDGF-AB, IGF-1 and VEGF.

公开(公告)号：US09914920B2

公开(公告)日：2018-03-13

申请号：US13859180

申请日：2013-04-09

申请人： Thomas J Goodwin ,  Moses J Kushman

发明人： Thomas J Goodwin ,  Moses J Kushman

IPC分类号： C12M1/00 ,  C12M3/00 ,  C12N13/00 ,  C12M3/04 ,  C12M1/42

CPC分类号： C12N13/00 ,  C12M23/24 ,  C12M23/28 ,  C12M27/10 ,  C12M27/20 ,  C12M29/04 ,  C12M35/02 ,  C12M35/04 ,  C12M35/06 ,  C12N5/0062 ,  C12N2513/00 ,  C12N2529/00

摘要： Provided herein are a culture apparatus, culture systems and an alternating ionic magnetic resonance electromagnetic chamber for culturing cells, tissues or organoid bodies or for delivering a pulsating alternating ionic magnetic resonance field to an object of interest including the above or to an animal, human or plant. The culture apparatus comprises a culture unit having growth and nutrient modules and a randomizing adapter to continually randomize the gravity vector in the growth module. The culture systems further comprise the alternating ionic magnetic resonance electromagnetic chamber.

公开(公告)号：US20180051252A1

公开(公告)日：2018-02-22

申请号：US15633283

申请日：2017-06-26

申请人： LUTERION CO., LTD.

发明人： Won Cheol Choi ,  Young Ah Kwon ,  Suk Hoon Choi ,  Chang Hoon Choi

IPC分类号： C12N5/078 ,  C12N5/076 ,  C12N13/00 ,  A61K35/14 ,  A61K35/52

CPC分类号： C12N5/0634 ,  A61K35/14 ,  A61K35/20 ,  A61K35/28 ,  A61K35/38 ,  A61K35/52 ,  C12N5/061 ,  C12N13/00 ,  C12N2529/00

摘要： The present disclosure relates to luterial, which is a mitochondrial-like unidentified nano-sized particle derived from a body fluid, and to a method for isolating the same.

公开(公告)号：US20170335291A1

公开(公告)日：2017-11-23

申请号：US15674337

申请日：2017-08-10

申请人： BELL BIOSYSTEMS, INC.

发明人： Caleb B. Bell, III ,  Alexey Bazarov ,  Brad Rice

IPC分类号： C12N5/26 ,  C12N13/00

CPC分类号： C12N5/166 ,  C12M35/06 ,  C12N13/00 ,  C12N2529/00

摘要： The present invention is directed generally to systems for making host cells with artificial endosymbionts. In an embodiment, the systems of the invention comprise a host cell, an artificial endosymbiont, a means for introducing the artificial endosymbiont to the host cell, a means for separating the host cells with artificial endosymbionts from free artificial endosymbionts, and a means for detecting artificial endosymbionts in host cells.

公开(公告)号：US20170304002A1

公开(公告)日：2017-10-26

申请号：US15507820

申请日：2015-09-02

申请人： Old Dominion University

发明人： Stephen J. BEEBE ,  Karl H. SCHOENBACH

IPC分类号： A61B18/20 ,  C12N15/70 ,  C12N15/10 ,  A61B18/18 ,  C12N5/00 ,  A61B18/00

CPC分类号： A61B18/20 ,  A61B18/18 ,  A61B2018/00994 ,  C12N5/00 ,  C12N15/101 ,  C12N15/70 ,  C12N2529/00

摘要： The present disclosure describes methods for intracellular electromanipulation of proteins using nanosecond pulsed electric fields (nsPEFs). The nsPEFs have effects on proteins in addition to permeabilizing cellular membranes. The nsPEFs induce a Ca2+-dependent dissipation of the mitochondria membrane potential (ΔΨm), which is enhanced when high frequency components are present in fast rise-fall waveforms. Ca2+ is shown to have little or no effect on propidium iodide uptake as a measure of plasma membrane poration and consequently intracellular membranes. Since Ca2+-regulated events are mediated by proteins, actions of nsPEFs on proteins that regulate and/or affect the mitochondria membrane potential are possible. Given that nsPEF-induced dissipation of ΔΨm was more effective when high frequency components were present in fast rise time waveforms, the effects on proteins are due to these high frequency components. These results present direct evidence that nsPEFs affect proteins and their functions by affecting their structure.

公开(公告)号：US20170106088A1

公开(公告)日：2017-04-20

申请号：US15315247

申请日：2015-06-03

申请人： UNIVERSITY OF HOUSTON SYSTEM ,  BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM

发明人： Dong LIU ,  Qilin CAO

IPC分类号： A61K41/00 ,  A61K35/33 ,  A61K35/15 ,  A61K47/02 ,  C12N5/00 ,  A61K9/107 ,  A61K47/34 ,  A61K38/18 ,  C12N5/0797 ,  C12N5/077 ,  A61K35/30 ,  A61K47/36

CPC分类号： A61K41/00 ,  A61K9/107 ,  A61K9/1272 ,  A61K35/15 ,  A61K35/30 ,  A61K35/33 ,  A61K38/185 ,  A61K47/02 ,  A61K47/34 ,  A61K47/36 ,  A61K47/6901 ,  A61K47/6923 ,  C12N5/0062 ,  C12N5/0623 ,  C12N5/0656 ,  C12N2529/00

摘要： The present disclosure relates to liposomal delivery vehicles comprising magnetic particles and their use in modifying stem cells for delivery to target sites such as neuronal tissues.