公开(公告)号：US20230374452A1

公开(公告)日：2023-11-23

申请号：US18030965

申请日：2021-10-06

Applicant: MiCAN Technologies Inc. ,  OSAKA UNIVERSITY

Inventor： Jun Shimizu ,  Naomi Tanga ,  Misuzu Yamada ,  Kazuo Miyazaki ,  Tatsuo Shioda ,  Emi Nakayama ,  Tadahiro Sasaki ,  Ritsuko Koketsu

IPC: C12N5/078 ,  C07K14/53 ,  C12N9/48 ,  C12Q1/70 ,  C12N5/10 ,  C12N7/02

CPC classification number: C12N5/0634 ,  C07K14/53 ,  C12N9/485 ,  C12Q1/70 ,  C12N5/10 ,  C12N7/02 ,  C12Y304/17023

Abstract: The present invention provides human cells that can be stably infected with coronavirus, as human cells for research on infection with coronavirus such as SARS-CoV-2, as well as, by utilizing the cells, a method for confirming the presence of a coronavirus in a sample, a method for producing a coronavirus, and a method for evaluating the presence or absence of anti-coronavirus activity of a test sample or a substance having an anti-coronavirus neutralizing action in a test sample. Specifically, the present invention relates to a method for preparing a coronavirus-infectious immortalized human myeloid cell, including introducing ACE2 and TMPRSS2 into an immortalized human myeloid cell, and the like.

公开(公告)号：US20150044225A1

公开(公告)日：2015-02-12

申请号：US14505515

申请日：2014-10-03

Applicant: Osaka University ,  The Research Foundation for Microbial Diseases of Osaka University

Inventor： Kazuyoshi Ikuta ,  Ritsuko Koketsu ,  Yoshinobu Okuno ,  Mikihiro Yunoki ,  Shoji Ideno ,  Masatoshi Oshita ,  Motoki Kuhara ,  Masatoshi Momota

IPC: C07K16/10

CPC classification number: C07K16/1018 ,  A61K2039/505 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/34 ,  C07K2317/76

Abstract: Provided is a human antibody having a neutralization activity against a human influenza virus. More specifically, provided is a human antibody which recognizes a highly conserved region in a human influenza A virus subtype H3N2 or a human influenza B virus and has a neutralization activity against the virus. The human antibody is a human anti-human influenza virus antibody, which has a neutralization activity against a human influenza A virus subtype H3N2 and binds to a hemagglutinin HA1 region of the human influenza A virus subtype H3N2, or which has a neutralization activity against a human influenza B virus, and includes, as a base sequence of a DNA encoding a variable region of the antibody, a sequence set forth in any one of SEQ ID NOS: 5 to 12.

Abstract translation: 本发明提供对人流感病毒具有中和活性的人抗体。 更具体地，提供了识别人流感A型病毒亚型H3N2或人类流感B型病毒中的高度保守区域并具有针对病毒的中和活性的人抗体。 人抗体是人抗流感病毒抗体，其对人甲型流感病毒亚型H3N2具有中和活性，并与人甲型流感病毒亚型H3N2的血凝素HA1区域结合，或者具有中和活性 人流感B病毒，并且作为编码抗体可变区的DNA的碱基序列，包含SEQ ID NO：5〜12中任一项所述的序列。

公开(公告)号：US09181328B2

公开(公告)日：2015-11-10

申请号：US14375675

申请日：2013-01-31

Applicant: OSAKA UNIVERSITY ,  THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY ,  MEDICAL AND BIOLOGICAL LABORATORIES CO., LTD. ,  DEPARTMENT OF MEDICAL SCIENCES

Inventor： Mayo Yasugi ,  Motoki Kuhara ,  Jotika Boon-Long ,  Kazuhito Fujiyama ,  Ritsuko Koketsu ,  Kazuyoshi Ikuta

IPC: C07K16/10 ,  G01N33/569 ,  A61K39/00

CPC classification number: C07K16/1018 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/54 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92 ,  G01N33/56983 ,  G01N2333/11

Abstract: Materials and methods are provided for treating influenza B infections in humans. Anti-human influenza virus monoclonal antibodies and antigen-binding fragments thereof having a neutralization activity against a human influenza B virus are provided. Methods for producing anti-human influenza B virus monoclonal antibodies are also provided. The antibodies and antigen-binding fragments thereof can be effective against a wide range of influenza B viral strains. Methods of inhibiting or treating a human influenza B infection are provided. The anti-influenza B therapeutics can also be used to manufacture medicaments effective against influenza B infections, to detect human influenza B in a human subject, for use in pharmaceutical compositions, and for use in kits for at least one of the prevention, the treatment, and the detection of human influenza B in a human subject.

Abstract translation: 提供了用于治疗人类B型流感病毒的材料和方法。 提供了抗人流感病毒单克隆抗体及其对人类B型流感病毒具有中和活性的抗原结合片段。 还提供了产生抗人B型流感病毒单克隆抗体的方法。 抗体及其抗原结合片段对于广泛的乙型流感病毒株是有效的。 提供了抑制或治疗人流感B感染的方法。 抗乙型流感病毒治疗剂也可用于制造有效抗B型流感病毒的药物，以检测人类受试者中的人类流感B，用于药物组合物，并用于至少一种预防，治疗 ，以及人类受试者中人类乙型流感的检测。

公开(公告)号：US20140377262A1

公开(公告)日：2014-12-25

申请号：US14375675

申请日：2013-01-31

Applicant: OSAKA UNIVERSITY ,  THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY ,  MEDICAL AND BIOLOGICAL LABORATORIES CO., LTD. ,  DEPARTMENT OF MEDICAL SCIENCES

Inventor： Mayo Yasugi ,  Motoki Kuhara ,  Jotika Boon-Long ,  Kazuhito Fujiyama ,  Ritsuko Koketsu ,  Kazuyoshi Ikuta

IPC: C07K16/10 ,  G01N33/569

CPC classification number: C07K16/1018 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/54 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92 ,  G01N33/56983 ,  G01N2333/11

Abstract: Materials and methods are provided for treating influenza B infections in humans. Anti-human influenza virus monoclonal antibodies and antigen-binding fragments thereof having a neutralization activity against a human influenza B virus are provided. Methods for producing anti-human influenza B virus monoclonal antibodies are also provided. The antibodies and antigen-binding fragments thereof can be effective against a wide range of influenza B viral strains. Methods of inhibiting or treating a human influenza B infection are provided. The anti-influenza B therapeutics can also be used to manufacture medicaments effective against influenza B infections, to detect human influenza B in a human subject, for use in pharmaceutical compositions, and for use in kits for at least one of the prevention, the treatment, and the detection of human influenza B in a human subject.

Abstract translation: 提供了用于治疗人类B型流感病毒的材料和方法。 提供了抗人流感病毒单克隆抗体及其对人类B型流感病毒具有中和活性的抗原结合片段。 还提供了产生抗人B型流感病毒单克隆抗体的方法。 抗体及其抗原结合片段对于广泛的乙型流感病毒株是有效的。 提供了抑制或治疗人流感B感染的方法。 抗乙型流感病毒治疗剂也可用于制造有效抗B型流感病毒的药物，以检测人类受试者中的人类流感B，用于药物组合物，并用于至少一种预防，治疗 ，以及人类受试者中人类乙型流感的检测。

公开(公告)号：US09493550B2

公开(公告)日：2016-11-15

申请号：US14505515

申请日：2014-10-03

Applicant: Osaka University ,  The Research Foundation for Microbial Diseases of Osaka University

Inventor： Kazuyoshi Ikuta ,  Ritsuko Koketsu ,  Yoshinobu Okuno ,  Mikihiro Yunoki ,  Shoji Ideno ,  Masatoshi Oshita ,  Motoki Kuhara ,  Masatoshi Momota

IPC: C07K16/10 ,  A61K39/00

CPC classification number: C07K16/1018 ,  A61K2039/505 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/34 ,  C07K2317/76

Abstract: Provided is a human antibody having a neutralization activity against a human influenza virus. More specifically, provided is a human antibody which recognizes a highly conserved region in a human influenza A virus subtype H3N2 or a human influenza B virus and has a neutralization activity against the virus. The human antibody is a human anti-human influenza virus antibody, which has a neutralization activity against a human influenza A virus subtype H3N2 and binds to a hemagglutinin HA1 region of the human influenza A virus subtype H3N2, or which has a neutralization activity against a human influenza B virus, and includes, as a base sequence of a DNA encoding a variable region of the antibody, a sequence set forth in any one of SEQ ID NOS: 5 to 12.