公开(公告)号：US20160176919A1

公开(公告)日：2016-06-23

申请号：US14978700

申请日：2015-12-22

申请人： Michael Cohen ,  Ruth Barrett ,  Haihong Jin

发明人： Michael Cohen ,  Ruth Barrett ,  Haihong Jin

IPC分类号： C07K1/13 ,  C07H19/16 ,  C12N9/84 ,  A01K67/027 ,  C12N15/85

CPC分类号： C07K1/13 ,  A01K67/0275 ,  A01K67/0278 ,  A01K2217/206 ,  A01K2227/105 ,  A01K2267/0362 ,  A01K2267/0393 ,  C07H19/16 ,  C12N9/84 ,  C12N15/8509 ,  C12Y305/01011

摘要： Disclosed herein are compositions comprising blocked puromycin analogs that are converted into active puromycin analogs upon the activity of a penicillin acylase. Also disclosed are methods of using blocked puromycin analogs to label proteins in a selected cell type in vivo in a transgenic multicellular organism that expresses a penicillin acylase within the selected cell type. Also disclosed are transgenic mice expressing a penicillin acylase within a selected cell type.

摘要翻译： 本文公开了包含封闭的嘌呤霉素类似物的组合物，其在青霉素酰基转移酶的活性上转化为活性嘌呤霉素类似物。 还公开了使用封闭的嘌呤霉素类似物在所选择的细胞类型中表达青霉素酰基转移酶的转基因多细胞生物体内在体内选择的细胞类型中标记蛋白质的方法。 还公开了在所选细胞类型内表达青霉素酰基转移酶的转基因小鼠。

公开(公告)号：US20070174925A1

公开(公告)日：2007-07-26

申请号：US10563628

申请日：2004-07-07

申请人： Ruth Barrett ,  Paul Goldsmith ,  Angeleen Fleming

发明人： Ruth Barrett ,  Paul Goldsmith ,  Angeleen Fleming

IPC分类号： A01K67/027

CPC分类号： G01N33/5088 ,  A01K67/0275 ,  A01K2227/40

摘要： The generation and use of disease models in fish such as zebrafish are provided, in particular use in a screen to identify a gene or test substance that affects a disease state associated with pain or nociception, or pain or nociception. In particular, fish models are used in a screen to identify a gene or test substance that affects activity or effect of a second gene provided transgenically that affects a pain or nociception state of the fish, or a treatment of the fish that affects a pain or nociception state of the fish. A pain state or nociception of model fish is compared with that of mutated or treated fish, allowing for identification of genes and test substances with therapeutic application.

摘要翻译： 提供了诸如斑马鱼等鱼类中疾病模型的产生和使用，特别是在筛选中用于鉴定影响与疼痛或伤害感受相关的疾病状态或疼痛或伤害感受的基因或测试物质。 特别地，在屏幕中使用鱼模型来鉴定影响转基因的影响鱼的疼痛或伤害感状态的第二基因的活性或作用的基因或测试物质，或影响鱼的治疗或影响疼痛的鱼 鱼的伤害感状态。 将模型鱼的疼痛状态或伤害感与突变或处理的鱼进行比较，从而允许用治疗应用鉴定基因和测试物质。