公开(公告)号：US20110313056A1

公开(公告)日：2011-12-22

申请号：US13184953

申请日：2011-07-18

申请人： Michael D. Buschmann ,  Dominic Filion ,  Marc Lavertu

发明人： Michael D. Buschmann ,  Dominic Filion ,  Marc Lavertu

IPC分类号： A61K47/36

CPC分类号： C08L5/08 ,  A61K8/19 ,  A61K8/736 ,  A61K41/0028 ,  A61K47/61 ,  A61K47/6903 ,  A61Q19/00 ,  C08B37/003

摘要： There is disclosed aqueous solutions of polyelectrolytes that can be neutralized by increasing temperature, under suitable polyelectrolyte charge state conditions, in order to obtain a homogeneous gel. This can be achieved by adding an appropriate weak electrolyte to the polyelectrolyte so that an increase of temperature will lead to a spatially homogeneous neutralization of the polyelectrolyte via proton transfer between the polyelectrolyte and the weak electrolyte. The ability of such a system to be thermally sensitive and to gel upon heating relies on the temperature dependence of the ionization equilibrium for the two components. This thermally induced neutralization reduces electrostatic repulsion between polyelectrolyte molecules allowing the manifestation of attractive polyelectrolyte-polyelectrolyte hydrophobic interactions and hydrogen bonding that result in gel formation. These new kinds of thermo sensitive gels can be used for biomedical applications.

摘要翻译： 公开了聚合电解质的水溶液，其可以在合适的聚电解质电荷状态条件下通过升高的温度中和，以获得均匀的凝胶。 这可以通过向聚电解质中添加适当的弱电解质来实现，使得温度的升高将导致聚电解质通过聚电解质和弱电解质之间的质子转移而空间均匀地中和。 这种系统在加热时热敏和凝胶的能力取决于两种组分的电离平衡的温度依赖性。 这种热诱导的中和降低了聚电解质分子之间的静电斥力，从而表现出导致凝胶形成的有吸引力的聚电解质 - 聚电解质疏水相互作用和氢键。 这些新型热敏凝胶可用于生物医学应用。

公开(公告)号：US20090149421A1

公开(公告)日：2009-06-11

申请号：US12092484

申请日：2006-11-06

申请人： Michael D. Buschmann ,  Dominic Filion ,  Marc Lavertu

发明人： Michael D. Buschmann ,  Dominic Filion ,  Marc Lavertu

IPC分类号： A61K31/722

CPC分类号： C08L5/08 ,  A61K8/19 ,  A61K8/736 ,  A61K41/0028 ,  A61K47/61 ,  A61K47/6903 ,  A61Q19/00 ,  C08B37/003

摘要： There is disclosed aqueous solutions of polyelectrolytes that can be neutralized by increasing temperature, under suitable polyelectrolyte charge state conditions, in order to obtain a homogeneous gel. This can be achieved by adding an appropriate weak electrolyte to the polyelectrolyte so that an increase of temperature will lead to a spatially homogeneous neutralization of the polyelectrolyte via proton transfer between the polyelectrolyte and the weak electrolyte. The ability of such a system to be thermally sensitive and to gel upon heating relies on the temperature dependence of the ionization equilibrium for the two components. This thermally induced neutralization reduces electrostatic repulsion between polyelectrolyte molecules allowing the manifestation of attractive polyelectrolyte-polyelectrolyte hydrophobic interactions and hydrogen bonding that result in gel formation. These new kinds of thermo sensitive gels can be used for biomedical applications.

摘要翻译： 公开了聚合电解质的水溶液，其可以在合适的聚电解质电荷状态条件下通过升高的温度中和，以获得均匀的凝胶。 这可以通过向聚电解质中添加适当的弱电解质来实现，使得温度的升高将导致聚电解质通过聚电解质和弱电解质之间的质子转移而空间均匀地中和。 这种系统在加热时热敏和凝胶的能力取决于两种组分的电离平衡的温度依赖性。 这种热诱导的中和降低了聚电解质分子之间的静电斥力，从而表现出导致凝胶形成的有吸引力的聚电解质 - 聚电解质疏水相互作用和氢键。 这些新型热敏凝胶可用于生物医学应用。

公开(公告)号：US20090075383A1

公开(公告)日：2009-03-19

申请号：US12092498

申请日：2006-11-06

申请人： Michael D. Buschmann ,  Marc Lavertu ,  Stephane Methot

发明人： Michael D. Buschmann ,  Marc Lavertu ,  Stephane Methot

IPC分类号： C12N15/85

CPC分类号： C12N15/87 ,  A61K31/70 ,  A61K31/715

摘要： There is disclosed a composition and a method for the efficient non-viral delivery of nucleic acids to cells using chitosan. In order to achieve high efficiency of transfection, the composition contains a nucleic acid and a chitosan that has the following physico-chemical properties: a combination of a number-average molecular weight between 8 kDa and 185 kDa and a degree of deacetylation between 72% and 92%. The chitosan molecule can also present additional physiochemical properties such as a block distribution of acetyl groups obtained by a heterogeneous treatment of chitin, and/or a polydispersity index between 1.4 and 7.0. By correctly controlling these parameters, efficient delivery systems may be produced that are effective when optimized for different conditions such as the pH of transfection media and amine-to-phosphate ratio.

摘要翻译： 公开了使用壳聚糖将核酸有效非病毒递送至细胞的组合物和方法。 为了实现高转染效率，组合物含有具有以下物理化学性质的核酸和壳聚糖：数均分子量在8kDa至185kDa之间，脱乙酰度为72％ 和92％。 壳聚糖分子还可以呈现另外的物理化学性质，例如通过几丁质的多相处理获得的乙酰基的嵌段分布和/或1.4-7.0之间的多分散性指数。 通过正确控制这些参数，可以产生有效的递送系统，其在针对不同条件如转染培养基的pH和胺与磷酸酯比例进行优化时是有效的。

公开(公告)号：US06332364B1

公开(公告)日：2001-12-25

申请号：US09553063

申请日：2000-04-20

申请人： Michael D. Buschmann ,  Martin Garon ,  Matthieu Ouellet ,  Marc Lavertu

发明人： Michael D. Buschmann ,  Martin Garon ,  Matthieu Ouellet ,  Marc Lavertu

IPC分类号： G01N300

CPC分类号： G01N3/08 ,  G01N3/20 ,  G01N33/15 ,  G01N2203/0071

摘要： A universal material testing device which includes a frame; an actuator mounted on the frame for controlling a displacement of a sample to be tested; a load cell movably mounted on the frame and adapted to abut against the sample for detecting a force applied thereon by the actuator, the load cell producing a signal corresponding to the force detected; a signal conditioning unit for reducing input noise and for processing of the signal and executing specific tests by coordination of displacement control and load signals received for processing from the load cell; and a detachable chamber with humidifying media for humidification of the sample environment confined within the chamber and separated frm the sample so as to avoid potential damaging effects of humidity on the sample or on the device. The device is useful for determining certain material properties of a sample.

摘要翻译： 一种通用材料测试装置，包括一个框架; 安装在框架上的致动器，用于控制待测样品的位移; 可移动地安装在所述框架上并且适于抵靠所述样本以用于检测由所述致动器施加在其上的力的测力传感器，所述测力传感器产生对应于所检测的力的信号; 信号调节单元，用于减少输入噪声和处理信号，并通过协调位移控制和从负载传感器处理接收的负载信号执行特定测试; 以及具有加湿介质的可拆卸室，用于加湿限定在室内的样品环境并分离出样品，以避免湿气对样品或装置的潜在的破坏性影响。 该装置对于确定样品的某些材料性质是有用的。

公开(公告)号：US20120321721A1

公开(公告)日：2012-12-20

申请号：US13510661

申请日：2010-11-18

申请人： Wei Ouyang ,  Michael Buschmann ,  Caroline Hoemann ,  Marc Lavertu ,  Anik Chevrier

发明人： Wei Ouyang ,  Michael Buschmann ,  Caroline Hoemann ,  Marc Lavertu ,  Anik Chevrier

IPC分类号： A61K35/14 ,  A61P27/02 ,  A61K35/50 ,  A61P19/08 ,  A61K35/28 ,  A61K35/44

CPC分类号： A61K35/14 ,  A61K35/28 ,  A61K35/51 ,  A61L27/3616 ,  A61L27/44 ,  C08L5/00 ,  C08L5/08 ,  C08L5/10

摘要： The present description relates to a polymer composition for use in repairing tissue of a patient comprising at least one blood component, a polymer, such as chitosan, and at least one inorganic salt, such as NaCl, method of using the composition and method of preparing the composition.

摘要翻译： 本说明书涉及用于修复患者组织的聚合物组合物，其包括至少一种血液成分，聚合物如壳聚糖和至少一种无机盐，例如NaCl，使用该组合物的方法和制备方法 组成。