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公开(公告)号：US07135565B2
公开(公告)日：2006-11-14
申请号：US09916369
申请日：2001-07-27
摘要： A method of synthesizing a polynucleotide which can, for example, be used during fabrication of an array. A second nucleoside is coupled to a first nucleoside through a phosphite linkage, with the second nucleoside having a hydroxyl protecting group that is a non-carbonate protecting group. The product of the foregoing step is exposed to a composition which both oxidizes the formed phosphite to a phosphate and deprotects the protected hydroxyl of the coupled nucleoside. The method has particular application to fabricating an addressable array of polynucleotides on a substrate which carries substrate bound moieties each with a hydroxyl group.
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公开(公告)号：US06451998B1
公开(公告)日：2002-09-17
申请号：US09420099
申请日：1999-10-18
IPC分类号： C07H2100
CPC分类号： C07H21/00 , C07B2200/11 , C40B40/00
摘要： A method of capping a hydroxy group of a moiety, comprising coupling the moiety to a phosphor or phosphite derivative of a protected alcohol, so as to form the corresponding phosphate or phosphite between the hydroxy and phosphor or phosphite groups. The hydroxy group may be later de-capped by hydrolyzing the resulting compound to deprotect the protected alcohol and cleave the phosphate from the moiety so as to regenerate the hydroxy group of the moiety. The method has particular application to fabrication of addressable polynucleotide arrays and allows failed sequences, as well as inter-feature regions, to be left with a free hydroxy group at the ends of the molecules (failed sequences or linkers) at such locations.
摘要翻译： 封端部分羟基的方法，包括将该部分偶合到被保护的醇的磷酸酯或亚磷酸酯衍生物上，以便在羟基和磷酸酯或亚磷酸酯基之间形成相应的磷酸酯或亚磷酸酯。 然后可以通过水解所得化合物来脱保护羟基，使保护的醇脱保护并从该部分切割磷酸盐，从而再生该部分的羟基。 该方法特别适用于可寻址的多核苷酸阵列的制造，并允许失效的序列以及特征间区域在这些位置处在分子的末端留下游离羟基（失败的序列或接头）。
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