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    Genetic Copy Number Determination Using High Throughput Multiplex Sequencing Of Smashed Nucleotides
    2.
    发明申请
    Genetic Copy Number Determination Using High Throughput Multiplex Sequencing Of Smashed Nucleotides 审中-公开

    公开(公告)号：US20170152548A1

    公开(公告)日：2017-06-01

    申请号：US15419878

    申请日：2017-01-30

    申请人： Michael H. Wigler ,  Dan Levy ,  Zihua Wang

    发明人： Michael H. Wigler ,  Dan Levy ,  Zihua Wang

    IPC分类号： C12Q1/68 ,  C12N15/10 ,  G06F19/22

    摘要： The present invention, SMASH (Short Multiply Aggregated Sequence Homologies), is a technique designed to pack multiple independent mappings into every read. Specifically, the invention relates to a composition comprising a first mixture of different chimeric genomic nucleic acid fragments, wherein each different fragment in the mixture comprises randomly ligated DNA segments, wherein each DNA segment in the fragment is a nucleic acid molecule at least 27 base pairs in length resulting from random fragmentation of a single genome. The invention also relates to methods for generating said composition and use of said composition to obtain genomic information, for example, copy number variation.

    Hamburger holder
    4.
    发明授权
    Hamburger holder 失效
    汉堡包持有人

    公开(公告)号：US4936479A

    公开(公告)日：1990-06-26

    申请号：US339513

    申请日：1989-04-17

    申请人： Dan Levy ,  George Spector

    发明人： Dan Levy ,  George Spector

    IPC分类号： A47G21/00

    CPC分类号： A47G21/001 ,  Y10S229/938

    摘要： A hamburger holder is provided and consists of a pair of hollow semi-cylindrical disc-shaped receptacles. The hamburger is stored within the receptacles until one is removed allowing a person to hold the remaining receptacle and eat the hamburger.

    摘要翻译： 提供汉堡包支架，由一对中空的半圆柱形盘形容器组成。 汉堡包储存在容器内，直至取出一个人，让一个人拿着剩余的容器并吃汉堡包。

    RANDOM NUCLEOTIDE MUTATION FOR NUCLEOTIDE TEMPLATE COUNTING AND ASSEMBLY
    6.
    发明申请
    RANDOM NUCLEOTIDE MUTATION FOR NUCLEOTIDE TEMPLATE COUNTING AND ASSEMBLY 有权

    公开(公告)号：US20210340604A1

    公开(公告)日：2021-11-04

    申请号：US17320634

    申请日：2021-05-14

    申请人： Michael Wigler ,  Dan Levy

    发明人： Michael Wigler ,  Dan Levy

    IPC分类号： C12Q1/6827 ,  C12Q1/6851 ,  C12Q1/6869 ,  C07H21/04 ,  C12N15/10

    摘要： A method for determining the number of nucleic acid molecules (NAMs) in a group of NAMs, comprising i) obtaining an amplified and mutagenized group of NAMs that was produced by a. subjecting the group of NAMs to a chemical mutagenesis which mutates only select nucleic acid bases in the group of NAMs at a rate of 10% to 90% thus forming a group of mutagenized NAMs (mNAMs), and b. amplifying the group of mNAMs; ii) obtaining sequences of the mNAMs in the group of amplified mNAMs; and iii) counting the number of different sequences obtained in step (ii) to determine the number of unique mNAMs in the group of mNAMS, thereby determining the number of NAMs in the group of NAMs.

    Random nucleotide mutation for nucleotide template counting and assembly
    7.
    发明授权
    Random nucleotide mutation for nucleotide template counting and assembly 有权

    公开(公告)号：US11008606B2

    公开(公告)日：2021-05-18

    申请号：US15515913

    申请日：2015-10-09

    申请人： Michael Wigler ,  Dan Levy

    发明人： Michael Wigler ,  Dan Levy

    IPC分类号： C12Q1/6827 ,  C12Q1/6851 ,  C12Q1/6869 ,  C07H21/04 ,  C12N15/10

    摘要： A method for determining the number of nucleic acid molecules (NAMs) in a group of NAMs, comprising i) obtaining an amplified and mutagenized group of NAMs that was produced by a. subjecting the group of NAMs to a chemical mutagenesis which mutates only select nucleic acid bases in the group of NAMs at a rate of 10% to 90% thus forming a group of mutagenized NAMs (mNAMs), and b. amplifying the group of mNAMs; ii) obtaining sequences of the mNAMs in the group of amplified mNAMs; and iii) counting the number of different sequences obtained in step (ii) to determine the number of unique mNAMs in the group of mNAMS, thereby determining the number of NAMs in the group of NAMs.