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公开(公告)号：US09834756B2
公开(公告)日：2017-12-05
申请号：US13183961
申请日：2011-07-15
CPC分类号： C12N5/0676 , A01N1/0247
摘要： Methods of isolating cellular products, such as pancreatic islets, may be used in diabetes research and therapeutic transplantation. The methods may involve providing a donor tissue having desired cells and undesired cells, perfusing the donor tissue with a perfusion solution, developing edema during perfusion of the donor tissue to form a swelled tissue, and separating the desired cells from undesired cellular material to obtain a cellular product. The methods may also include disrupting the tissue, and separating the desired cells from undesired cellular material to obtain the cellular product. The methods may result in an increased yield of cellular product that retains sufficient functional integrity to be useful as a transplantation resource.
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公开(公告)号：US09963679B2
公开(公告)日：2018-05-08
申请号：US13278492
申请日：2011-10-21
申请人： Michael J. Taylor , Simona C. Baicu
发明人： Michael J. Taylor , Simona C. Baicu
IPC分类号： C12N5/071
CPC分类号： C12N5/0677 , C12N2500/00
摘要： Methods of preparing pancreatic islets that may comprise pancreatites are provided. The methods may involve subjecting a pancreas and/or pieces thereof to disruption, such as, for example, an enzyme digest, and seeding the recovered cellular product comprising islets in a culture medium comprising at least a detectable amount of endocrine tissue and/or exocrine tissue.
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