公开(公告)号：US10030245B2

公开(公告)日：2018-07-24

申请号：US13427138

申请日：2012-03-22

申请人： Michael Lassner ,  Derek Jantz ,  James Jefferson Smith ,  Mark Cigan ,  Carl Falco ,  Huirong Gao ,  Zhongsen Li ,  Zhan-Bin Liu ,  Sergei Svitashev

发明人： Michael Lassner ,  Derek Jantz ,  James Jefferson Smith ,  Mark Cigan ,  Carl Falco ,  Huirong Gao ,  Zhongsen Li ,  Zhan-Bin Liu ,  Sergei Svitashev

IPC分类号： C12N15/82 ,  A01H1/06 ,  C12N9/22

摘要： Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.

公开(公告)号：US20130263324A1

公开(公告)日：2013-10-03

申请号：US13427138

申请日：2012-03-22

申请人： Michael Lassner ,  Derek Jantz ,  James Jefferson Smith ,  Mark Cigan ,  Carl Falco ,  Huirong Gao ,  Zhongsen Li ,  Zhan-Bin Liu ,  Sergei Svitashev

发明人： Michael Lassner ,  Derek Jantz ,  James Jefferson Smith ,  Mark Cigan ,  Carl Falco ,  Huirong Gao ,  Zhongsen Li ,  Zhan-Bin Liu ,  Sergei Svitashev

IPC分类号： C12N15/82

CPC分类号： C12N15/8202 ,  A01H1/06 ,  C12N9/22 ,  C12N15/8213

摘要： Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.

摘要翻译： 公开了在植物中生产复合转基因性状基因座的方法，所述基因座包含在目的基因组区域中的至少两个改变的靶序列。 所述方法包括使用两种或更多种双链断裂诱导剂，其中每一种可引起目的基因组区域中靶序列中的双链断裂，导致目标序列的改变。 还公开了植物中的复杂的转基因性状基因座。 复杂的转基因性状基因座包含与感兴趣的多核苷酸遗传连锁的至少两个改变的靶序列。 还公开了植物，植物细胞，植物部分和包含一个或多个复合转基因性状基因座的种子。

公开(公告)号：US20150184194A1

公开(公告)日：2015-07-02

申请号：US14129038

申请日：2012-06-19

申请人： Dennis L. Bidney ,  Mark Cigan ,  Carl Falco ,  Huirong Gao ,  Derek Jantz ,  Mike Lassner ,  Keith Lowe ,  Leszek Aleksander Lyznik ,  James Jefferson Smith

发明人： Dennis L. Bidney ,  Mark Cigan ,  Carl Falco ,  Huirong Gao ,  Derek Jantz ,  Mike Lassner ,  Keith Lowe ,  Leszek Aleksander Lyznik ,  James Jefferson Smith

IPC分类号： C12N15/82

CPC分类号： C12N15/8289 ,  C12N5/14 ,  C12N9/22 ,  C12N15/8213 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Y301/00

摘要： Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.

摘要翻译： 公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。 所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。 还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。 包含其中具有至少一个靶向修饰的雄性能育基因的核酸分子，编码工程化双链断裂诱导剂和表达盒的内切核酸酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物 进一步公开。

公开(公告)号：US09574208B2

公开(公告)日：2017-02-21

申请号：US14129038

申请日：2012-06-19

申请人： Dennis L. Bidney ,  Mark Cigan ,  Carl Falco ,  Huirong Gao ,  Derek Jantz ,  Mike Lassner ,  Keith Lowe ,  Leszek Aleksander Lyznik ,  James Jefferson Smith

发明人： Dennis L. Bidney ,  Mark Cigan ,  Carl Falco ,  Huirong Gao ,  Derek Jantz ,  Mike Lassner ,  Keith Lowe ,  Leszek Aleksander Lyznik ,  James Jefferson Smith

IPC分类号： C12N15/82 ,  C12N5/14

CPC分类号： C12N15/8289 ,  C12N5/14 ,  C12N9/22 ,  C12N15/8213 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Y301/00

摘要： Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.

摘要翻译： 公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。 所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。 还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。 包含其中具有至少一个靶向修饰的雄性能育基因的核酸分子，编码工程化双链断裂诱导剂和表达盒的内切核酸酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物 进一步公开。