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公开(公告)号：US20100173776A1
公开(公告)日：2010-07-08
申请号：US12443317
申请日：2007-09-28
申请人： Michael Schade , Dieter Hofer , Christoph Grimm , Domingos Pedroni , Paulo Aramaki , Eva Krupkova , Thomas Schmulling , Andy Cobb
发明人： Michael Schade , Dieter Hofer , Christoph Grimm , Domingos Pedroni , Paulo Aramaki , Eva Krupkova , Thomas Schmulling , Andy Cobb
摘要： A method of increasing functional protein activity in a plant comprising applying to the plant, part of the plant, the plant propagation material thereof and/or a surrounding area thereof an effective amount of one or more neonicotinoid compounds selected from thianicotinyl compounds and tetrahydrofuranyl compounds.
摘要翻译： 一种提高植物功能蛋白活性的方法，包括向植物，部分植物，植物繁殖材料和/或其周围区域施用有效量的一种或多种选自噻嗪基化合物和四氢呋喃基化合物的新烟碱类化合物。
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公开(公告)号：US20090222948A1
公开(公告)日：2009-09-03
申请号：US12291376
申请日：2008-11-07
申请人： Thomas Schmulling , Tomas Werner
发明人： Thomas Schmulling , Tomas Werner
CPC分类号： C12N9/0032 , C12N15/8261 , C12N15/8271 , C12N15/8287 , C12N15/8295 , Y02A40/146
摘要： The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. Also provided by the present invention are methods for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a cytokinin oxidase or expression of another protein that reduces the level of active cytokinins in plants or plant parts. Methods and compositions for increasing seed yield are also provided. The invention also relates to isolated plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising such sequences. The use of these sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology, is also provided. The invention also relates to methods for identifying and obtaining proteins and compounds interacting with cytokinin oxidase proteins as well as the use of such proteins and/or compounds as plant growth regulators or herbicides.
摘要翻译： 本发明涉及用于刺激根生长和/或增强侧根或不定根形成和/或改变根向地力的方法，包括细胞分裂素氧化酶的表达或包括降低植物或植物中活性细胞分裂素水平的另一种蛋白质的表达 部分。 本发明还提供了增加种子大小和/或重量，胚胎大小和/或重量以及子叶大小和/或重量的方法。 该方法包括细胞分裂素氧化酶的表达或另一种降低植物或植物部分活性细胞分裂素水平的蛋白质的表达。 还提供了用于提高种子产量的方法和组合物。 本发明还涉及分离的植物细胞分裂素氧化酶蛋白，编码细胞分裂素氧化酶蛋白的核酸序列以及包含这些序列的载体，宿主细胞，转基因细胞和植物。 这些序列用于改善根相关特征，包括增加产量和/或增强早期活力和/或改善根/芽比，和/或改善抗倒伏和/或增加耐旱性和/或促进外植体的体外繁殖 和/或修饰细胞命运和/或植物发育和/或植物形态和/或植物生物化学和/或植物生理学。 本发明还涉及用于鉴定和获得与细胞分裂素氧化酶蛋白相互作用的蛋白质和化合物以及使用这些蛋白质和/或化合物作为植物生长调节剂或除草剂的方法。
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公开(公告)号：US20050150012A1
公开(公告)日：2005-07-07
申请号：US10498168
申请日：2002-12-10
申请人： Thomas Schmulling , Tomas Werner
发明人： Thomas Schmulling , Tomas Werner
CPC分类号： C12N9/0032 , C12N15/8295
摘要： The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a plant cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. Also provided by the present invention are methods for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a plant cytokinin oxidase or expression of another protein that reduces the level of active cytokinins in plants or plant parts. The invention also relates to novel plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising said sequences. The invention also relates to the use of said sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology. The invention also relates to the use of said sequences in the above-mentioned methods. The invention also relates to methods for identifying and obtaining proteins and compounds interacting with cytokinin oxidase proteins. The invention also relates to the use of said compounds as a plant growth regulator or herbicide.
摘要翻译： 本发明涉及用于刺激根生长和/或增强侧根或不定根的形成和/或改变根地貌的方法，包括植物细胞分裂素氧化酶的表达或包括降低植物中活性细胞分裂素水平的另一种蛋白质的表达或 植物部位。 本发明还提供了增加种子大小和/或重量，胚胎大小和/或重量以及子叶大小和/或重量的方法。 所述方法包括植物细胞分裂素氧化酶的表达或降低植物或植物部分中活性细胞分裂素水平的另一种蛋白质的表达。 本发明还涉及新型植物细胞分裂素氧化酶蛋白，编码细胞分裂素氧化酶蛋白的核酸序列以及包含所述序列的载体，宿主细胞，转基因细胞和植物。 本发明还涉及所述序列用于改善根相关特征的用途，包括增加产量和/或增强早期活力和/或改善根/芽比率和/或改善抗倒伏和/或增加耐旱性和/或促进作用 外植体的体外繁殖和/或修饰细胞命运和/或植物发育和/或植物形态和/或植物生物化学和/或植物生理学。 本发明还涉及上述方法中所述序列的用途。 本发明还涉及用于鉴定和获得与细胞分裂素氧化酶蛋白相互作用的蛋白质和化合物的方法。 本发明还涉及所述化合物作为植物生长调节剂或除草剂的用途。
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公开(公告)号：US20120311742A1
公开(公告)日：2012-12-06
申请号：US13469872
申请日：2012-05-11
申请人： Thomas Schmulling , Tomàs Werner
发明人： Thomas Schmulling , Tomàs Werner
IPC分类号： C12N15/82
CPC分类号： C12N9/0032 , C12N15/8261 , C12N15/8271 , C12N15/8287 , C12N15/8295 , Y02A40/146
摘要： The present invention relates to methods for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a cytokinin oxidase in plants or plant parts, preferably in seeds, embryos and/or cotyledons. The methods result in increases in: yield, growth of seedlings, early vigor, and stress tolerance. The invention also relates to isolated plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising such sequences.
摘要翻译： 本发明涉及增加种子大小和/或重量，胚胎大小和/或重量以及子叶大小和/或重量的方法。 所述方法包括在植物或植物部分中表达细胞分裂素氧化酶，优选在种子，胚胎和/或子叶中表达。 这种方法导致：产量，幼苗生长，早期活力和胁迫耐受性增加。 本发明还涉及分离的植物细胞分裂素氧化酶蛋白，编码细胞分裂素氧化酶蛋白的核酸序列以及包含这些序列的载体，宿主细胞，转基因细胞和植物。
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公开(公告)号：US20080222755A1
公开(公告)日：2008-09-11
申请号：US11801018
申请日：2007-05-08
申请人： Thomas Schmulling , Tomas Werner
发明人： Thomas Schmulling , Tomas Werner
CPC分类号： C12N15/8261 , C12N9/0032 , C12N15/8271 , C12N15/8287 , C12N15/8295 , Y02A40/146
摘要： The present invention provides nucleotide sequences and corresponding amino acid sequences for plant cytokinin oxidase proteins. In addition, vectors, host cells, and transgenic plants comprising such sequences as well as methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism using such sequences are provided by the present invention. Also provided by the present invention are methods for altering various plant phenotypes including delaying onset to flowering, increasing leaf thickness, reducing vessel size, inducing parthenocarpy, increasing branching, increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight using cytokinin oxidase proteins and/or nucleic acid molecules encoding cytokinin oxidase.
摘要翻译： 本发明提供植物细胞分裂素氧化酶蛋白质的核苷酸序列和相应的氨基酸序列。 此外，本发明提供了包含这些序列的载体，宿主细胞和转基因植物以及用于刺激根生长和/或增强侧向或不定根形成和/或使用这种序列改变根向上性的方法。 本发明还提供了改变各种植物表型的方法，包括延迟开花开始，增加叶厚度，减少血管大小，诱导单性结实，增加分枝，增加种子大小和/或重量，胚胎大小和/或重量，以及子叶 使用细胞分裂素氧化酶蛋白和/或编码细胞分裂素氧化酶的核酸分子的大小和/或重量。
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公开(公告)号：US20050223429A1
公开(公告)日：2005-10-06
申请号：US11057473
申请日：2005-02-14
CPC分类号： C12N9/0032 , C12N15/8229 , C12N15/823 , C12N15/8261 , C12N15/827 , Y02A40/146
摘要： The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. Also provided by the present invention are methods for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a cytokinin oxidase or expression of another protein that reduces the level of active cytokinins in plants or plant parts. Methods and compositions for increasing seed yield are also provided. The invention also relates to isolated plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising such sequences. The use of these sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology, is also provided. The invention also relates to methods for identifying and obtaining proteins and compounds interacting with cytokinin oxidase proteins as well as the use of such proteins and/or compounds as plant growth regulators or herbicides.
摘要翻译： 本发明涉及用于刺激根生长和/或增强侧根或不定根形成和/或改变根向地力的方法，包括细胞分裂素氧化酶的表达或包括降低植物或植物中活性细胞分裂素水平的另一种蛋白质的表达 部分。 本发明还提供了增加种子大小和/或重量，胚胎大小和/或重量以及子叶大小和/或重量的方法。 该方法包括细胞分裂素氧化酶的表达或另一种降低植物或植物部分活性细胞分裂素水平的蛋白质的表达。 还提供了用于提高种子产量的方法和组合物。 本发明还涉及分离的植物细胞分裂素氧化酶蛋白，编码细胞分裂素氧化酶蛋白的核酸序列以及包含这些序列的载体，宿主细胞，转基因细胞和植物。 这些序列用于改善根相关特征，包括增加产量和/或增强早期活力和/或改善根/芽比，和/或改善抗倒伏和/或增加耐旱性和/或促进外植体的体外繁殖 和/或修饰细胞命运和/或植物发育和/或植物形态和/或植物生物化学和/或植物生理学。 本发明还涉及用于鉴定和获得与细胞分裂素氧化酶蛋白相互作用的蛋白质和化合物以及使用这些蛋白质和/或化合物作为植物生长调节剂或除草剂的方法。
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公开(公告)号：US08859854B2
公开(公告)日：2014-10-14
申请号：US13539876
申请日：2012-07-02
申请人： Thomas Schmulling , Tomas Werner
发明人： Thomas Schmulling , Tomas Werner
CPC分类号： C12N15/8261 , C12N9/0032 , C12N15/8271 , C12N15/8287 , C12N15/8295 , Y02A40/146
摘要： The present invention provides methods and compositions for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a cytokinin oxidase or expression of another protein that reduces the level of active cytokinins in plants or plant parts. Methods and compositions for increasing seed yield are also provided. The invention also relates to isolated plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising such sequences.
摘要翻译： 本发明提供了用于增加种子大小和/或重量，胚胎大小和/或重量以及子叶大小和/或重量的方法和组合物。 该方法包括细胞分裂素氧化酶的表达或另一种降低植物或植物部分活性细胞分裂素水平的蛋白质的表达。 还提供了用于提高种子产量的方法和组合物。 本发明还涉及分离的植物细胞分裂素氧化酶蛋白，编码细胞分裂素氧化酶蛋白的核酸序列以及包含这些序列的载体，宿主细胞，转基因细胞和植物。
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公开(公告)号：US08766038B2
公开(公告)日：2014-07-01
申请号：US11803820
申请日：2007-05-16
申请人： Thomas Schmulling , Tomas Werner
发明人： Thomas Schmulling , Tomas Werner
CPC分类号： C12N15/8261 , C12N9/0032 , C12N15/8271 , C12N15/8287 , C12N15/8295 , Y02A40/146
摘要： The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a plant cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. The invention also relates to novel plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising said sequences. The invention also relates to the use of said sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology. The invention also relates to the use of said sequences in the above-mentioned methods. The invention also relates to methods for identifying and obtaining proteins and compounds interacting with cytokinin oxidase proteins. The invention also relates to the use of said compounds as a plant growth regulator or herbicide.
摘要翻译： 本发明涉及用于刺激根生长和/或增强侧根或不定根的形成和/或改变根地貌的方法，包括植物细胞分裂素氧化酶的表达或包括降低植物中活性细胞分裂素水平的另一种蛋白质的表达或 植物部位。 本发明还涉及新型植物细胞分裂素氧化酶蛋白，编码细胞分裂素氧化酶蛋白的核酸序列以及包含所述序列的载体，宿主细胞，转基因细胞和植物。 本发明还涉及所述序列用于改善根相关特征的用途，包括增加产量和/或增强早期活力和/或改善根/芽比率和/或改善抗倒伏和/或增加耐旱性和/或促进作用 外植体的体外繁殖和/或修饰细胞命运和/或植物发育和/或植物形态和/或植物生物化学和/或植物生理学。 本发明还涉及上述方法中所述序列的用途。 本发明还涉及用于鉴定和获得与细胞分裂素氧化酶蛋白相互作用的蛋白质和化合物的方法。 本发明还涉及所述化合物作为植物生长调节剂或除草剂的用途。
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9.发明申请SUBSTITUTED 6-ANILINOPURINE DERIVATIVES AS INHIBITORS OF CYTOKININ OXIDASE/DEHYDROGENASE AND PREPARATIONS CONTAINING THESE DERIVATIVES 有权作为细胞因子氧化酶/脱氢酶抑制剂和含有这些衍生物的制剂的6-硝基嘌呤衍生物公开(公告)号：US20100190806A1
公开(公告)日：2010-07-29
申请号：US12666189
申请日：2008-07-02
申请人： Lukas Spichal , Marketa Gemrotova , Marek Zatloukal , Jitka Frebortova , Petr Galuszka , Tomas Werner , Thomas Schmulling , Karel Dolezal , Miroslav Strnad
发明人： Lukas Spichal , Marketa Gemrotova , Marek Zatloukal , Jitka Frebortova , Petr Galuszka , Tomas Werner , Thomas Schmulling , Karel Dolezal , Miroslav Strnad
IPC分类号： A61K31/52 , C07D473/00
CPC分类号： C07D473/16 , A01N43/90 , C07D473/34
摘要： The invention relates to substituted 6-anilinopurine derivatives of the general formula I, wherein R denotes one to five substituents independently selected from the group consisting of hydrogen, halogen, hydroxyl, amino, alkyloxy and alkyl group, and R2 denotes amino, halogen, nitro, thio, alkylthio or alkyl group for use as inhibitors of cytokinin oxidase/dehydrogenase. The invention also relates to the compositions containing these derivatives.
摘要翻译： 本发明涉及通式I的取代的6-苯胺基嘌呤衍生物，其中R表示一至五个独立地选自氢，卤素，羟基，氨基，烷氧基和烷基的取代基，R2表示氨基，卤素，硝基 ，硫代，烷硫基或烷基，用作细胞分裂素氧化酶/脱氢酶的抑制剂。 本发明还涉及含有这些衍生物的组合物。
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公开(公告)号：US20050044594A1
公开(公告)日：2005-02-24
申请号：US10871304
申请日：2004-06-18
申请人： Thomas Schmulling , Tomas Werner
发明人： Thomas Schmulling , Tomas Werner
CPC分类号： C12N9/0032 , C12N15/8261 , C12N15/8271 , C12N15/8287 , C12N15/8295 , Y02A40/146
摘要： The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. Also provided by the present invention are methods for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a cytokinin oxidase or expression of another protein that reduces the level of active cytokinins in plants or plant parts. Methods and compositions for increasing seed yield are also provided. The invention also relates to isolated plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising such sequences. The use of these sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology, is also provided. The invention also relates to methods for identifying and obtaining proteins and compounds interacting with cytokinin oxidase proteins as well as the use of such proteins and/or compounds as plant growth regulators or herbicides.
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