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1.
公开(公告)号:US20080207541A1
公开(公告)日:2008-08-28
申请号:US10592919
申请日:2005-03-15
申请人: Michael T. Migawa , Walter F. Lima , Eric E. Swayze , Joshua Nichols , Hongjiang Wu , Thazha P. Prakash , Tadeusz Krzysztof Wyrzykiewicz , Balkrishen Bhat , Stanley T. Crooke
发明人: Michael T. Migawa , Walter F. Lima , Eric E. Swayze , Joshua Nichols , Hongjiang Wu , Thazha P. Prakash , Tadeusz Krzysztof Wyrzykiewicz , Balkrishen Bhat , Stanley T. Crooke
CPC分类号: C12N15/85 , C12N15/111 , C12N2310/11 , C12N2310/315 , C12N2310/318 , C12N2310/321 , C12N2310/346 , C12N2310/3519 , C12N2310/3525
摘要: The present invention provides compositions and methods for the optimization of cleavage of RNA species by RNase H. In some embodiments, the invention provides oligonucleotides that possess two or more regions of differing conformation, and at least one transitional nucleobase positioned between the regions that is capable of modulating transfer of the helical conformation characteristic of the region bound to the 3′hydroxy thereof, to the region bound to the 5′ hydroxyl thereof.
摘要翻译: 本发明提供了通过RNA酶H优化RNA种类的切割的组合物和方法。在一些实施方案中,本发明提供具有不同构象的两个或更多个区域的寡核苷酸,以及位于能够区分的区域之间的至少一个过渡核碱基 调节与其3'-羟基结合的区域的螺旋构象特征转移到与其5'羟基结合的区域。
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2.
公开(公告)号:US08790919B2
公开(公告)日:2014-07-29
申请号:US10592919
申请日:2005-03-15
申请人: Michael T. Migawa , Walter F. Lima , Eric E. Swayze , Joshua Nichols , Hongjiang Wu , Thazha P. Prakash , Tadeusz Krzysztof Wyrzykiewicz , Balkrishen Bhat , Stanley T. Crooke
发明人: Michael T. Migawa , Walter F. Lima , Eric E. Swayze , Joshua Nichols , Hongjiang Wu , Thazha P. Prakash , Tadeusz Krzysztof Wyrzykiewicz , Balkrishen Bhat , Stanley T. Crooke
CPC分类号: C12N15/85 , C12N15/111 , C12N2310/11 , C12N2310/315 , C12N2310/318 , C12N2310/321 , C12N2310/346 , C12N2310/3519 , C12N2310/3525
摘要: The present invention provides compositions and methods for the optimization of cleavage of RNA species by RNase H. In some embodiments, the invention provides oligonucleotides that possess two or more regions of differing conformation, and at least one transitional nucleobase positioned between the regions that is capable of modulating transfer of the helical conformation characteristic of the region bound to the 3′hydroxy thereof, to the region bound to the 5′ hydroxyl thereof.
摘要翻译: 本发明提供了通过RNA酶H优化RNA种类的切割的组合物和方法。在一些实施方案中,本发明提供具有不同构象的两个或更多个区域的寡核苷酸,以及位于能够区分的区域之间的至少一个过渡核碱基 调节与其3'-羟基结合的区域的螺旋构象特征转移到与其5'羟基结合的区域。
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