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1.发明授权Gapped oligomeric compounds having linked bicyclic sugar moieties at the termini 有权在末端具有连接的双环糖部分的缺口低聚化合物公开(公告)号：US08703728B2
公开(公告)日：2014-04-22
申请号：US10935958
申请日：2004-09-08
IPC分类号： C12N15/113
CPC分类号： C07H21/04
摘要： The present invention relates to bicyclic nucleosides and oligomeric compounds comprising at least one such nucleoside. These oligomeric compounds typically have enhanced binding affinity and nuclease resistance properties compared to unmodified oligomeric compounds. The oligomeric compounds are useful, for example, for investigative and therapeutic purposes.
摘要翻译： 本发明涉及包含至少一种这样的核苷的双环核苷和寡聚化合物。 与未改性的低聚化合物相比，这些寡聚化合物通常具有增强的结合亲和力和核酸酶抗性。 寡聚化合物例如用于调查和治疗目的是有用的。
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2.发明申请公开(公告)号：US20080207541A1
公开(公告)日：2008-08-28
申请号：US10592919
申请日：2005-03-15
申请人： Michael T. Migawa , Walter F. Lima , Eric E. Swayze , Joshua Nichols , Hongjiang Wu , Thazha P. Prakash , Tadeusz Krzysztof Wyrzykiewicz , Balkrishen Bhat , Stanley T. Crooke
发明人： Michael T. Migawa , Walter F. Lima , Eric E. Swayze , Joshua Nichols , Hongjiang Wu , Thazha P. Prakash , Tadeusz Krzysztof Wyrzykiewicz , Balkrishen Bhat , Stanley T. Crooke
CPC分类号： C12N15/85 , C12N15/111 , C12N2310/11 , C12N2310/315 , C12N2310/318 , C12N2310/321 , C12N2310/346 , C12N2310/3519 , C12N2310/3525
摘要： The present invention provides compositions and methods for the optimization of cleavage of RNA species by RNase H. In some embodiments, the invention provides oligonucleotides that possess two or more regions of differing conformation, and at least one transitional nucleobase positioned between the regions that is capable of modulating transfer of the helical conformation characteristic of the region bound to the 3′hydroxy thereof, to the region bound to the 5′ hydroxyl thereof.
摘要翻译： 本发明提供了通过RNA酶H优化RNA种类的切割的组合物和方法。在一些实施方案中，本发明提供具有不同构象的两个或更多个区域的寡核苷酸，以及位于能够区分的区域之间的至少一个过渡核碱基 调节与其3'-羟基结合的区域的螺旋构象特征转移到与其5'羟基结合的区域。
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公开(公告)号：US08513404B2
公开(公告)日：2013-08-20
申请号：US12989268
申请日：2009-04-24
摘要： A process for manufacturing an oligonucleotide which comprises removing β-eliminating phosphorus-protecting groups, in particular β-cyanoethyl protective groups from a protected oligonucleotide, wherein said removing comprises contacting the protected oligonucleotide with an amine solution in a solvent which preferably does not consist of pyridine, wherein the conjugate acid of the amine has preferably a pKa of greater than 11.5, and wherein the concentration of the amine in the solution is less than 0.5 mole/liters.
摘要翻译： 制备寡核苷酸的方法，其包括从受保护的寡核苷酸中除去β-消除磷保护基，特别是β-氰基乙基保护基，其中所述除去包括使受保护的寡核苷酸与优选不由 吡啶，其中胺的共轭酸优选具有大于11.5的pKa，并且其中溶液中胺的浓度小于0.5摩尔/升。
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公开(公告)号：US08790919B2
公开(公告)日：2014-07-29
申请号：US10592919
申请日：2005-03-15
申请人： Michael T. Migawa , Walter F. Lima , Eric E. Swayze , Joshua Nichols , Hongjiang Wu , Thazha P. Prakash , Tadeusz Krzysztof Wyrzykiewicz , Balkrishen Bhat , Stanley T. Crooke
发明人： Michael T. Migawa , Walter F. Lima , Eric E. Swayze , Joshua Nichols , Hongjiang Wu , Thazha P. Prakash , Tadeusz Krzysztof Wyrzykiewicz , Balkrishen Bhat , Stanley T. Crooke
CPC分类号： C12N15/85 , C12N15/111 , C12N2310/11 , C12N2310/315 , C12N2310/318 , C12N2310/321 , C12N2310/346 , C12N2310/3519 , C12N2310/3525
摘要： The present invention provides compositions and methods for the optimization of cleavage of RNA species by RNase H. In some embodiments, the invention provides oligonucleotides that possess two or more regions of differing conformation, and at least one transitional nucleobase positioned between the regions that is capable of modulating transfer of the helical conformation characteristic of the region bound to the 3′hydroxy thereof, to the region bound to the 5′ hydroxyl thereof.
摘要翻译： 本发明提供了通过RNA酶H优化RNA种类的切割的组合物和方法。在一些实施方案中，本发明提供具有不同构象的两个或更多个区域的寡核苷酸，以及位于能够区分的区域之间的至少一个过渡核碱基 调节与其3'-羟基结合的区域的螺旋构象特征转移到与其5'羟基结合的区域。
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公开(公告)号：US20110087014A1
公开(公告)日：2011-04-14
申请号：US12989268
申请日：2009-04-24
IPC分类号： C07H1/00
摘要： A process for manufacturing an oligonucleotide which comprises removing β-eliminating phosphorus-protecting groups, in particular β-cyanoethyl protective groups from a protected oligonucleotide, wherein said removing comprises contacting the protected oligonucleotide with an amine solution in a solvent which preferably does not consist of pyridine, wherein the conjugate acid of the amine has preferably a pKa of greater than 11.5, and wherein the concentration of the amine in the solution is less than 0.5 mole/liters.
摘要翻译： 一种制备寡核苷酸的方法，其包括从被保护的寡核苷酸中去除并消除并保护磷保护基团，特别是β-氰基乙基保护基团，其中所述去除包括将受保护的寡核苷酸与胺溶液接触，所述溶液优选不溶于 由吡啶组成，其中胺的共轭酸优选具有大于11.5的pKa，并且其中溶液中胺的浓度小于0.5摩尔/升。
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