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公开(公告)号:US07615354B2
公开(公告)日:2009-11-10
申请号:US10275041
申请日:2001-05-04
申请人: Michel D. Faupel , Hubert H. Girault , Frederic Reymond , Alexandra Ros , Joel Stephane Rossier
发明人: Michel D. Faupel , Hubert H. Girault , Frederic Reymond , Alexandra Ros , Joel Stephane Rossier
IPC分类号: G01N33/53
CPC分类号: G01N27/44795 , G01N27/44769 , G01N2030/0035 , G01N2030/285
摘要: A device, method and kit for the electrophoretic separation and purification of charged and neutral compounds in an analyte solution. The device comprises a chamber (1), at least one wall of which is composed of a chemical buffering system (4). A potential difference is applied across the buffering system, resulting in the charged and neutral compounds being differentially separated by extraction of the charged compounds into the buffering system. The device also comprises means for collecting the separated compounds, preferably in ampholyte-free or buffer-free solution and optionally means for recycling the separated fractions.
摘要翻译: 用于在分析物溶液中电荷分离和纯化带电和中性化合物的装置,方法和试剂盒。 该装置包括室(1),其至少一个壁由化学缓冲系统(4)组成。 在缓冲系统上施加电位差,导致带电和中性化合物通过将带电荷的化合物萃取到缓冲系统中而被差异地分离。 该装置还包括用于收集分离的化合物的装置,优选以不含两性电解质或不含缓冲液的溶液和用于再循环分离的级分的方法。