-
公开(公告)号:US07026528B2
公开(公告)日:2006-04-11
申请号:US10210370
申请日:2002-07-31
申请人: Ming Cheng , Joyce E. Fry , Yeuchun Wan
发明人: Ming Cheng , Joyce E. Fry , Yeuchun Wan
CPC分类号: C12N15/8205
摘要: Disclosed are processes for producing stably transformed fertile wheat a system of transforming wheat via Agrobacterium. This invention provides methods transforming a variety of explants, such as freshly isolated or pre-cultured immature embryos, embryogenic callus and suspension cells. Also disclosed are methods for recovering transgenic plants after transformation within a short period of time, if the explants are regenerable at the time of transformation. Thus the frequency of somaclonal variation associated with prolonged in vitro culture period is significantly reduced. The transformation frequency using this system is comparable to or better than published methods using other systems, such as microprojectile bombardment.
-
公开(公告)号:US07045357B2
公开(公告)日:2006-05-16
申请号:US09459022
申请日:1999-12-10
申请人: Ming Cheng , Joyce E. Fry
发明人: Ming Cheng , Joyce E. Fry
CPC分类号: C12N15/8205
摘要: The present invention relates to a rapid transformation and regeneration system for plants. In particular, the invention relates to a plant tissue preparation system. The transformation method is efficient and reliable for production of fertile plants with improved agronomic qualities.
摘要翻译: 本发明涉及植物快速转化和再生系统。 特别地,本发明涉及植物组织制备系统。 这种转化方法对于生产具有改良农艺性状的肥沃植物是有效可靠的。
-
公开(公告)号:US07238862B2
公开(公告)日:2007-07-03
申请号:US10064849
申请日:2002-08-22
申请人: Helen Allison , Xiaorong Feng , Joyce E. Fry , Tianci Hu , Fengming Lu , Maxim Radionenko
发明人: Helen Allison , Xiaorong Feng , Joyce E. Fry , Tianci Hu , Fengming Lu , Maxim Radionenko
CPC分类号: C12N15/8205
摘要: The present invention relates to an improved transformation and regeneration system for wheat. In particular, the invention relates to enhancements in the glyphosate selection system. The transformation method is efficient and reliable for production of fertile plants with improved agronomic qualities.
摘要翻译: 本发明涉及一种改进的小麦转化和再生系统。 特别地,本发明涉及草甘膦选择系统中的增强。 这种转化方法对于生产具有改良农艺性状的肥沃植物是有效可靠的。
-
公开(公告)号:US09353377B2
公开(公告)日:2016-05-31
申请号:US13480462
申请日:2012-05-24
申请人: Yinghui Dan , Joyce E. Fry
发明人: Yinghui Dan , Joyce E. Fry
IPC分类号: C12N15/82
CPC分类号: C12N15/8207 , C12N15/8205
摘要: Mesocotyl meristem explants that contain multiple primary meristems are transformed via particle bombardment or Agrobacterium-mediated methods. Regeneration is through an organogenesis pathway that allows for secondary multiple bud formation. This method allows for the genotype independent transformation of varieties of wheat.
摘要翻译: 含有多个原生分生组织的中胚轴分生组织外植体通过粒子轰击或农杆菌介导的方法转化。 再生是通过器官发生途径,允许二次多芽形成。 这种方法允许小麦品种的基因型独立转化。
-
公开(公告)号:US20120297506A1
公开(公告)日:2012-11-22
申请号:US13480462
申请日:2012-05-24
申请人: Yinghui Dan , Joyce E. Fry
发明人: Yinghui Dan , Joyce E. Fry
IPC分类号: A01H1/00
CPC分类号: C12N15/8207 , C12N15/8205
摘要: Mesocotyl meristem explants that contain multiple primary meristems are transformed via particle bombardment or Agrobacterium-mediated methods. Regeneration is through an organogenesis pathway that allows for secondary multiple bud formation. This method allows for the genotype independent transformation of varieties of wheat.
摘要翻译: 含有多个原生分生组织的中胚轴分生组织外植体通过粒子轰击或农杆菌介导的方法转化。 再生是通过器官发生途径,允许二次多芽形成。 这种方法允许小麦品种的基因型独立转化。
-
公开(公告)号:US08212109B2
公开(公告)日:2012-07-03
申请号:US10064001
申请日:2002-06-03
申请人: Yinghui Dan , Joyce E. Fry
发明人: Yinghui Dan , Joyce E. Fry
CPC分类号: C12N15/8207 , C12N15/8205
摘要: Mesocotyl meristem explants that contain multiple primary meristems are transformed via particle bombardment or Agrobacterium-mediated methods. Regeneration is through an organogenesis pathway that allows for secondary multiple bud formation. This method allows for the genotype independent transformation of varieties of wheat.
摘要翻译: 含有多个原生分生组织的中胚轴分生组织外植体通过粒子轰击或农杆菌介导的方法转化。 再生是通过器官发生途径,允许二次多芽形成。 这种方法允许小麦品种的基因型独立转化。
-
公开(公告)号:US5631152A
公开(公告)日:1997-05-20
申请号:US329742
申请日:1994-10-26
申请人: Joyce E. Fry , Hua-ping Zhou
发明人: Joyce E. Fry , Hua-ping Zhou
CPC分类号: C12N15/8201 , C12N15/8207 , C12N15/8209
摘要: A rapid transformation regeneration system is disclosed. This system takes two-three months to obtain transgenic plants. Transformation efficiencies are very high. This system also has been demonstrated with several different selecting systems and is particularly useful for transforming wheat.
摘要翻译: 公开了一种快速转化再生系统。 该系统需要两三个月才能获得转基因植物。 转型效率非常高。 该系统也已经用几种不同的选择系统进行了证明,特别适用于转化小麦。
-
-
-
-
-
-