公开(公告)号：US20130307029A1

公开(公告)日：2013-11-21

申请号：US13989771

申请日：2011-12-31

申请人： Mingsheng Xu ,  Hongzheng Chen ,  Gang Wu ,  Minmin Shi ,  Mang Wang

发明人： Mingsheng Xu ,  Hongzheng Chen ,  Gang Wu ,  Minmin Shi ,  Mang Wang

IPC分类号： G01N27/414

CPC分类号： G01N27/4145

摘要： A high-resolution biosensor for analysis of biomolecules is provided. The high-resolution biosensor comprises a functional unit comprising a conducting material with an atomic-scale thickness and a micro-nano fluidic system unit. The functional unit is capable of achieving a resolution required to detect a characteristic of individual biomolecule, and the micro-nano fluidic system unit is capable of controlling the movement and conformation of the biomolecule investigated. The functional unit comprises a first insulating layer, conducting functional layer, a second insulating layer, and a nanopore extending through the full thickness of the functional unit. The micro-nano fluidic system unit comprises a first electrophoresis electrode or micropump, a first fluidic reservoir, a second fluidic reservoir, a second electrophoresis electrode or micropump, and micro-nanometer separation channels. The nanopore connects to the micro-nanometer separation channels. Interactions between the biomolecule and conducting functional layer occur as the biomolecule translocates through the nanopore of the functional unit.

摘要翻译： 提供了用于分析生物分子的高分辨率生物传感器。 高分辨率生物传感器包括具有原子尺度厚度的导电材料和微纳米流体系统单元的功能单元。 该功能单元能够实现检测各个生物分子的特征所需的分辨率，微纳米流体系统单元能够控制所研究的生物分子的运动和构象。 功能单元包括第一绝缘层，导电功能层，第二绝缘层和延伸穿过功能单元的整个厚度的纳米孔。 微纳米流体系统单元包括第一电泳电极或微型泵，第一流体储存器，第二流体储存器，第二电泳电极或微型泵以及微纳米分离通道。 纳米孔连接到微纳米分离通道。 当生物分子通过功能单元的纳米孔移位时，生物分子和导电功能层之间的相互作用发生。