公开(公告)号：US20130307029A1

公开(公告)日：2013-11-21

申请号：US13989771

申请日：2011-12-31

申请人： Mingsheng Xu ,  Hongzheng Chen ,  Gang Wu ,  Minmin Shi ,  Mang Wang

发明人： Mingsheng Xu ,  Hongzheng Chen ,  Gang Wu ,  Minmin Shi ,  Mang Wang

IPC分类号： G01N27/414

CPC分类号： G01N27/4145

摘要： A high-resolution biosensor for analysis of biomolecules is provided. The high-resolution biosensor comprises a functional unit comprising a conducting material with an atomic-scale thickness and a micro-nano fluidic system unit. The functional unit is capable of achieving a resolution required to detect a characteristic of individual biomolecule, and the micro-nano fluidic system unit is capable of controlling the movement and conformation of the biomolecule investigated. The functional unit comprises a first insulating layer, conducting functional layer, a second insulating layer, and a nanopore extending through the full thickness of the functional unit. The micro-nano fluidic system unit comprises a first electrophoresis electrode or micropump, a first fluidic reservoir, a second fluidic reservoir, a second electrophoresis electrode or micropump, and micro-nanometer separation channels. The nanopore connects to the micro-nanometer separation channels. Interactions between the biomolecule and conducting functional layer occur as the biomolecule translocates through the nanopore of the functional unit.

摘要翻译： 提供了用于分析生物分子的高分辨率生物传感器。 高分辨率生物传感器包括具有原子尺度厚度的导电材料和微纳米流体系统单元的功能单元。 该功能单元能够实现检测各个生物分子的特征所需的分辨率，微纳米流体系统单元能够控制所研究的生物分子的运动和构象。 功能单元包括第一绝缘层，导电功能层，第二绝缘层和延伸穿过功能单元的整个厚度的纳米孔。 微纳米流体系统单元包括第一电泳电极或微型泵，第一流体储存器，第二流体储存器，第二电泳电极或微型泵以及微纳米分离通道。 纳米孔连接到微纳米分离通道。 当生物分子通过功能单元的纳米孔移位时，生物分子和导电功能层之间的相互作用发生。

公开(公告)号：US20120037919A1

公开(公告)日：2012-02-16

申请号：US13266540

申请日：2010-09-14

申请人： Ming-sheng Xu ,  Hongzheng Chen ,  Minmin Shi ,  Gang Wu ,  Mang Wang

发明人： Ming-sheng Xu ,  Hongzheng Chen ,  Minmin Shi ,  Gang Wu ,  Mang Wang

IPC分类号： H01L29/66 ,  H01L29/16 ,  H01L29/20

CPC分类号： G01N33/48721 ,  B82Y15/00 ,  C12Q1/6869 ,  C12Q2565/631

摘要： A nanopore electrical sensor is provided. The sensor has layered structure, including a substrate (1), the first insulating layer (2), a symmetrical electrode (3) and the second insulating layer (5) from bottom to top in turn. A nanopore (6) is provided in the center of the substrate (1), the first insulating layer (2), the symmetrical electrode (3) and the second insulating layer (5). The thickness of the symmetrical electrode can be controlled between 0.3 nm and 0.7 nm so as to meet the resolution requirements for detecting a single base in a single-stranded DNA. Thus the sensor is suitable for gene sequencing. The present invention overcomes current technical insufficiency to integrate a nanoelectrode with a nanopore and the method to prepare the nanoelectrode is simple.

摘要翻译： 提供纳米孔电传感器。 该传感器依次具有层叠结构，包括基底（1），第一绝缘层（2），对称电极（3）和第二绝缘层（5）。 在基板（1），第一绝缘层（2），对称电极（3）和第二绝缘层（5）的中心设置有纳米孔（6）。 可以将对称电极的厚度控制在0.3nm和0.7nm之间，以满足检测单链DNA中单个碱基的分辨率要求。 因此，传感器适用于基因测序。 本发明克服了当前纳米电极纳米电极的技术不足，制备纳米电极的方法简单。

公开(公告)号：US20130037410A1

公开(公告)日：2013-02-14

申请号：US13643296

申请日：2011-09-24

申请人： Ming-Sheng Xu ,  Hongzheng Chen ,  Gang Wu ,  Minmin Shi ,  Mang Wang

发明人： Ming-Sheng Xu ,  Hongzheng Chen ,  Gang Wu ,  Minmin Shi ,  Mang Wang

IPC分类号： G01N27/453

CPC分类号： B82Y15/00 ,  G01N27/447 ,  G01N33/48721

摘要： A nanopore sensor comprises second electrophoresis electrode or micropump, second fluidic reservoir, second micro-nanometer separation channel, substrate, sub-nanometer-thick functional layer, first micro-nanometer separation channel, first electrophoresis electrode or micropump, and electrophoresis electrode or micropump that are sequentially assembled. An opening and a nanopore are provided through the substrate and the sub-nanometer-thick functional layer, respectively. A first electrode for measuring ionic current is provided in the first micro-nanometer separation channel, and a second electrode for measuring ionic current is provide in the second micro-nanometer separation channel. The present invention provides a simple method to prepare a sub-nanometer functional layer having a nanopore extending through the sub-nanometer-thick functional layer. The pore size is comparable to the spacing between two adjacent bases in a DNA strand required for single-base resolution sequencing. The shape of nanopore overcomes nucleotide conformation effect on the identification as bases translocate through the nanopore.

摘要翻译： 纳米孔传感器包括第二电泳电极或微泵，第二流体储存器，第二微纳米分离通道，衬底，亚纳米厚功能层，第一微纳米分离通道，第一电泳电极或微型泵以及电泳电极或微型泵， 顺序组装。 分别通过基底和亚纳米厚的功能层设置开口和纳米孔。 在第一微纳米分离通道中提供用于测量离子电流的第一电极，并且在第二微纳米分离通道中提供用于测量离子电流的第二电极。 本发明提供了制备具有延伸穿过亚纳米厚功能层的纳米孔的亚纳米功能层的简单方法。 孔径可以与单碱基拆分测序所需的DNA链中两个相邻碱基之间的间距相当。 纳米孔的形状克服了通过纳米孔易位的基因鉴定的核苷酸构象影响。

公开(公告)号：US09133023B2

公开(公告)日：2015-09-15

申请号：US13643296

申请日：2011-09-24

申请人： Ming-Sheng Xu ,  Hongzheng Chen ,  Gang Wu ,  Minmin Shi ,  Mang Wang

发明人： Ming-Sheng Xu ,  Hongzheng Chen ,  Gang Wu ,  Minmin Shi ,  Mang Wang

IPC分类号： G01N27/453 ,  B82Y15/00 ,  G01N33/487 ,  G01N27/447

CPC分类号： B82Y15/00 ,  G01N27/447 ,  G01N33/48721

摘要： A nanopore sensor comprises second electrophoresis electrode or micropump, second fluidic reservoir, second micro-nanometer separation channel, substrate, sub-nanometer-thick functional layer, first micro-nanometer separation channel, first electrophoresis electrode or micropump, and first electrophoresis electrode or micropump that are sequentially assembled. An opening and a nanopore are provided through the substrate and the sub-nanometer-thick functional layer, respectively. A first electrode for measuring ionic current is provided in the first micro-nanometer separation channel, and a second electrode for measuring ionic current is provide in the second micro-nanometer separation channel. The present invention provides a simple method to prepare a sub-nanometer functional layer having a nanopore extending through the sub-nanometer-thick functional layer. The pore size is comparable to the spacing between two adjacent bases in a DNA strand required for single-base resolution sequencing. The shape of nanopore overcomes nucleotide conformation effect on the identification as bases translocate through the nanopore.

摘要翻译： 纳米孔传感器包括第二电泳电极或微泵，第二流体储存器，第二微纳米分离通道，衬底，亚纳米厚功能层，第一微纳米分离通道，第一电泳电极或微型泵，以及第一电泳电极或微泵 顺序组装。 分别通过基底和亚纳米厚的功能层设置开口和纳米孔。 在第一微纳米分离通道中提供用于测量离子电流的第一电极，并且在第二微纳米分离通道中提供用于测量离子电流的第二电极。 本发明提供了制备具有延伸穿过亚纳米厚功能层的纳米孔的亚纳米功能层的简单方法。 孔径可以与单碱基拆分测序所需的DNA链中两个相邻碱基之间的间距相当。 纳米孔的形状克服了通过纳米孔易位的基因鉴定的核苷酸构象影响。