公开(公告)号：US20120162754A1

公开(公告)日：2012-06-28

申请号：US13334195

申请日：2011-12-22

申请人： Mirko LIEDTKE

发明人： Mirko LIEDTKE

IPC分类号： G02B21/06

CPC分类号： G02B21/006 ,  G02B21/008

摘要： A laser scanning microscope comprising having a light source, an illuminating beam for illuminating a specimen with a spatially limited, preferably point-shaped, illuminating light spot via a scanning device and an objective lens, and a detection beam for detecting the specimen light that reaches the detection beam via the objective lens and the scanning device. Focusing means that are disposed in the detection beam and focus the light from the specimen to form a spatially limited detection light spot in a plane. Receiver elements are distributed in the spot in a matrix-like manner and can be individually read out. These are located in order to simulate an adjustable pinhole aperture.

摘要翻译： 一种激光扫描显微镜，包括：具有光源的光源，用于通过扫描装置和物镜用空间有限的优选点状的照明光点照射样本的照明光束，以及用于检测到达到的样本光的检测光束 通过物镜和扫描装置的检测光束。 聚焦装置设置在检测光束中并聚焦来自样品的光，以在平面中形成空间有限的检测光斑。 接收器元件以矩阵状的方式分布在现场，并可以单独读出。 这些位置是为了模拟可调针孔。

公开(公告)号：US20110119034A1

公开(公告)日：2011-05-19

申请号：US12970019

申请日：2010-12-16

申请人： Mirko LIEDTKE ,  Frank KLEMM ,  Manfred LOTH

发明人： Mirko LIEDTKE ,  Frank KLEMM ,  Manfred LOTH

IPC分类号： G06F15/00

CPC分类号： G02B21/0076 ,  G01J3/2889 ,  G01J3/4406

摘要： A method for recording pulse signals which allows the reconstruction of a time reference. The time of every pulse signal event can be determined by counting sampling result bits preceding the respective sampling result bit using the known sampling frequency. For this purpose, every period of the sampling frequency is associated with a bit representing the respective sampling result and the sampling result bits are stored one by one and per channel in data blocks. The sampling frequency is preferably higher than a pixel clock, a sampling result bit associated with a flank of the pixel clock being marked. The pixel clock can thus be synchronized with the individual events exactly per sampling period. The invention further relates to the field of fluorescence correlation spectroscopy using confocal microscopes or laser scanning microscopes.

摘要翻译： 一种用于记录允许重建时间基准的脉冲信号的方法。 可以通过使用已知的采样频率对相应采样结果位之前的采样结果位进行计数来确定每个脉冲信号事件的时间。 为此，采样频率的每个周期都与表示相应采样结果的位相关联，采样结果位逐个存储，每个通道存储在数据块中。 采样频率优选地高于像素时钟，与被标记的像素时钟的侧面相关联的采样结果位。 因此，像素时钟可以在每个采样周期精确地与各个事件同步。 本发明还涉及使用共焦显微镜或激光扫描显微镜的荧光相关光谱领域。

公开(公告)号：US20110149388A1

公开(公告)日：2011-06-23

申请号：US12970007

申请日：2010-12-16

申请人： Mirko LIEDTKE ,  Johannes WEBER

发明人： Mirko LIEDTKE ,  Johannes WEBER

IPC分类号： G02B21/06 ,  H01J40/14

CPC分类号： G02B21/008 ,  H01J43/30

摘要： A operating circuit and control method for protecting a PMT having a photocathode, a plurality of dynodes and an anode against overloading with a shorter reaction time, and to allow it to be switched on again rapidly. For this purpose, a switch is provided for electrically short circuiting the photocathode with the first dynode, or a switch is provided for reversing the polarity of the voltage between the photocathode and the first dynode.

摘要翻译： 一种用于保护具有光电阴极的PMT，多个倍增电极和阳极的操作电路和控制方法，可以在较短的反应时间内抵抗过载，并允许其快速接通。 为此，提供了用于使光电阴极与第一倍增电极短路的开关，或提供用于反转光电阴极和第一倍增极之间的电压的极性的开关。

公开(公告)号：US20110032614A1

公开(公告)日：2011-02-10

申请号：US12849967

申请日：2010-08-04

申请人： Mirko LIEDTKE ,  Matthias John

发明人： Mirko LIEDTKE ,  Matthias John

IPC分类号： G01N25/02 ,  G02B21/00

CPC分类号： G02B21/362 ,  G01J1/02 ,  G01J1/0252 ,  G02B7/008 ,  G02B21/28

摘要： An optoelectronic detector and method of using same. In order to avoid any condensation on a surface, it has been known to heat such a surface. However, heating an optoelectronic detector will create a stronger hissing noise due to the greater dark current that is caused thereby. The invention is intended to avoid any condensation on an optoelectronic detector without airtight encapsulation. To this end, the detector is cooled and equipped with a sensor for the determination of a current value of one of the variables ambient humidity and ambient dew point temperature and a control unit that is connected with the sensor and designed to control the cooling device in dependence of such a value. By taking into account the ambient humidity or, respectively, the dew point temperature in the control of the cooling device, condensation on the detector can be avoided. An airtight encapsulation of the detector and the cooling device is not required.

摘要翻译： 一种光电检测器及其使用方法。 为了避免表面上的任何冷凝，已知加热这种表面。 然而，由于由此引起的较大的暗电流，加热光电检测器将产生更强的嘶嘶声。 本发明旨在避免光电检测器上没有气密封装的任何冷凝。 为此，检测器被冷却并配备有用于确定环境湿度和环境露点温度之一的变量的当前值的传感器，以及与传感器连接并被设计成控制冷却装置的控制单元 这种价值的依赖。 通过考虑环境湿度或分别控制冷却装置的露点温度，可以避免检测器上的冷凝。 不需要检测器和冷却装置的气密封装。