Alkaline protease
    1.
    发明申请
    Alkaline protease 有权
    碱性蛋白酶

    公开(公告)号:US20050214922A1

    公开(公告)日:2005-09-29

    申请号:US10820714

    申请日:2004-04-09

    CPC分类号: C12N9/54 C11D3/386

    摘要: An alkaline protease having an amino acid sequence, wherein one or more amino acid residues selected from those located at (a) position 15, (b) position 16, (c) position 166, (d) position 167, (e) position 187, (f) position 346, and (g) position 405 of the amino acid sequence of SEQ ID NO: 1, or at positions corresponding to these positions are the following amino acid residues, respectively: (a): histidine, (b): threonine or glutamine, (c): glycine, (d) valine, (e): serine, (f): arginine, and (g): aspartic acid; and a gene encoding the alkaline protease. The alkaline protease exhibits for example excellent detergency against complex soil, and improved protein secretion amount and specific activity, and attains enhanced productivity.

    摘要翻译: 一种具有氨基酸序列的碱性蛋白酶,其中选自位于(a)位置15,(b)位置16,(c)位置166,(d)位置167,(e)位置187的氨基酸残基中的一个或多个氨基酸残基 ,(f)位置346和(g)SEQ ID NO:1的氨基酸序列的位置405或对应于这些位置的位置分别是以下氨基酸残基:(a):组氨酸,(b) :苏氨酸或谷氨酰胺,(c):甘氨酸,(d)缬氨酸,(e):丝氨酸,(f):精氨酸,和(g):天冬氨酸; 和编码碱性蛋白酶的基因。 碱性蛋白酶表现出对复杂土壤的优异的去污力,以及提高的蛋白质分泌量和比活性,并且提高了生产率。

    Alkaline protease
    2.
    发明授权
    Alkaline protease 有权
    碱性蛋白酶

    公开(公告)号:US07429642B2

    公开(公告)日:2008-09-30

    申请号:US10820714

    申请日:2004-04-09

    IPC分类号: C07K1/00

    CPC分类号: C12N9/54 C11D3/386

    摘要: An alkaline protease having an amino acid with one or more amino acid residues selected from among those located at (a) position 15, (b) position 16, (c) position 166, (d) position 167, (e) position 187, (f) position 346, and (g) position 405 of the amino acid sequence of SEQ No; 1, or at positions corresponding to these positions, are the following amino acid residues, respectively: (a): histidine, (b): threonine or glutamine, (c): glycine, (d): valine, (e): serine, (f): arginine, and (g); and a gene encoding the alkaline protease.

    摘要翻译: 一种具有一个或多个氨基酸残基的氨基酸的碱性蛋白酶,其选自位于(a)位置15,(b)位置16,(c)位置166,(d)位置167,(e)位置187, (f)位置346和(g)SEQ ID NO:1的氨基酸序列的位置405; 分别为:(a):组氨酸,(b):苏氨酸或谷氨酰胺,(c):甘氨酸,(d):缬氨酸,(e)):丝氨酸 ,(f):精氨酸和(g); 和编码碱性蛋白酶的基因。

    Alkaline protease
    4.
    发明授权
    Alkaline protease 有权
    碱性蛋白酶

    公开(公告)号:US07101698B2

    公开(公告)日:2006-09-05

    申请号:US10456479

    申请日:2003-06-09

    CPC分类号: C07H21/04 C12N9/54

    摘要: The present invention relates to an alkaline protease having a prepro sequence, wherein the prepro sequence is a mutant sequence of the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having 80% or higher homology to the amino acid sequence of SEQ ID NO: 1, in which amino acid residues at (a) position 52, (b) position 75, and (c) position 142 of SEQ ID NO: 1, or amino acid residues at positions corresponding to these positions are substituted by the following amino acid residues: at position (a): aspartic acid or arginine, at position (b): alanine or arginine, and at position (c): lysine; and the alkaline protease, when in a mature form, has the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having a homology of 80% or higher to this amino acid sequence. The present invention also relates to, for example, a gene encoding the alkaline protease. According to the present invention, an alkaline protease increased in production can be produced. In particular, there can be efficiently produced an alkaline protease having an activity even in the presence of a fatty acid of high concentration and exhibiting excellent detergency against complex soils containing proteins and sebum.

    摘要翻译: 本发明涉及具有前序列的碱性蛋白酶,其中前序列是SEQ ID NO:1的氨基酸序列的突变序列或与SEQ ID NO:1的氨基酸序列具有80%以上同源性的氨基酸序列 SEQ ID NO:1的(a)位置52,(b)位置75和(c)位置142处的氨基酸残基或对应于这些位置的位置处的氨基酸残基被替换为SEQ ID NO: 以下氨基酸残基:位置(a):天冬氨酸或精氨酸,位置(b):丙氨酸或精氨酸,和位置(c):赖氨酸; 碱性蛋白酶以成熟形式具有SEQ ID NO:2的氨基酸序列或与该氨基酸序列同源性为80%以上的氨基酸序列。 本发明还涉及例如编码碱性蛋白酶的基因。 根据本发明,可以生产生产中增加的碱性蛋白酶。 特别是,即使在高浓度的脂肪酸的存在下也能够有效地生成具有活性的碱性蛋白酶,并且对于含有蛋白质和皮脂的复杂土壤表现出优异的去污力。

    Alkaline protease
    8.
    发明授权
    Alkaline protease 有权
    碱性蛋白酶

    公开(公告)号:US07368273B2

    公开(公告)日:2008-05-06

    申请号:US10385662

    申请日:2003-03-12

    IPC分类号: C12N9/48 C11D3/386

    摘要: An alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 17-0, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO: 2 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (e): histidine, aspartic acid, phenylalanine, lysine, asparagine, seine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (K): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): seine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine. A method to an alkaline protease having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore useful as an ingredient in a detergent.

    摘要翻译: (a)位置65,(b)位置101,(c)位置163,(d)位置17-0,(e)位置171,(f)位置273,(g) 位置320,(h)位置359或(i)SEQ ID NO: 位置(a):脯氨酸,位置(b):天冬酰胺,位置(e):组氨酸,天冬氨酸,苯丙氨酸,赖氨酸,天冬酰胺, (e):丙氨酸,谷氨酸,甘氨酸和苏氨酸,位置(K):异亮氨酸,甘氨酸和苏氨酸,位置(g):缬氨酸和亮氨酸, (i):丙氨酸,赖氨酸,谷氨酰胺,谷氨酸,精氨酸和组氨酸,位置(h):ine碱,亮氨酸,缬氨酸,异亮氨酸和谷氨酰胺。 一种碱性蛋白酶的方法,即使在高度浓缩的脂肪酸的存在下也具有活性,并且对于去除含有蛋白质,皮脂等的复合污渍具有优异的去污力,因此可用作洗涤剂中的成分。

    Alkaline protease
    9.
    发明授权
    Alkaline protease 有权
    碱性蛋白酶

    公开(公告)号:US07776578B2

    公开(公告)日:2010-08-17

    申请号:US12049022

    申请日:2008-03-14

    摘要: Provided in the present invention is an alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 170, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO:1 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (c): histidine, aspartic acid, phenylalanine, lysine, asparagine, serine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (f): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): serine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine.The present invention makes it possible to efficiently produce and provide alkaline proteases having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore being useful as an enzyme to be incorporated in a detergent.

    摘要翻译: 在本发明中提供的是碱性蛋白酶,其中(a)位置65,(b)位置101,(c)位置163,(d)位置170,(e)位置171,(f) ,(g)位置320,(h)位置359或(i)SEQ ID NO: 位置(a):脯氨酸,位置(b):天冬酰胺,位置(c):组氨酸,天冬氨酸,苯丙氨酸,赖氨酸,天冬酰胺, 位置(d):缬氨酸和亮氨酸,位置(e):丙氨酸,谷氨酸,甘氨酸和苏氨酸,位置(f):异亮氨酸,甘氨酸和苏氨酸,位置(g) 位置(h):丝氨酸,亮氨酸,缬氨酸,异亮氨酸和谷氨酰胺,位置(i):丙氨酸,赖氨酸,谷氨酰胺,谷氨酸,精氨酸和组氨酸,位置(h):缬氨酸,缬氨酸,苏氨酸,亮氨酸,异亮氨酸和甘氨酸。 本发明使得可以有效地生产和提供具有活性的碱性蛋白酶,即使在高度浓缩的脂肪酸的存在下也具有优异的去污力来去除含有蛋白质,皮脂等的复合污渍,因此可用作 要掺入洗涤剂中的酶。

    ALKALINE PROTEASE
    10.
    发明申请
    ALKALINE PROTEASE 有权
    碱性蛋白酶

    公开(公告)号:US20080177040A1

    公开(公告)日:2008-07-24

    申请号:US12049022

    申请日:2008-03-14

    IPC分类号: C07K2/00

    摘要: Provided in the present invention is an alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 170, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO:1 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (c): histidine, aspartic acid, phenylalanine, lysine, asparagine, serine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (f): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): serine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine.The present invention makes it possible to efficiently produce and provide alkaline proteases having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore being useful as an enzyme to be incorporated in a detergent.

    摘要翻译: 在本发明中提供的是碱性蛋白酶,其中(a)位置65,(b)位置101,(c)位置163,(d)位置170,(e)位置171,(f) ,(g)位置320,(h)位置359或(i)SEQ ID NO: 位置(a):脯氨酸,位置(b):天冬酰胺,位置(c):组氨酸,天冬氨酸,苯丙氨酸,赖氨酸,天冬酰胺, 位置(d):缬氨酸和亮氨酸,位置(e):丙氨酸,谷氨酸,甘氨酸和苏氨酸,位置(f):异亮氨酸,甘氨酸和苏氨酸,位置(g) 位置(h):丝氨酸,亮氨酸,缬氨酸,异亮氨酸和谷氨酰胺,位置(i):丙氨酸,赖氨酸,谷氨酰胺,谷氨酸,精氨酸和组氨酸,位置(h):缬氨酸,缬氨酸,苏氨酸,亮氨酸,异亮氨酸和甘氨酸。 本发明使得可以有效地生产和提供具有活性的碱性蛋白酶,即使在高度浓缩的脂肪酸的存在下也具有优异的去污力来去除含有蛋白质,皮脂等的复合污渍,因此可用作 要掺入洗涤剂中的酶。