公开(公告)号：US20220298516A1

公开(公告)日：2022-09-22

申请号：US17276983

申请日：2019-09-20

Applicant: ModernaTX, Inc.

Inventor： Aaron LARSEN ,  Jennifer NELSON ,  Melissa MOORE

IPC: C12N15/67 ,  C12N15/88 ,  A61K47/00 ,  A61K31/7088 ,  C12N15/11

Abstract: The present disclosure relates to methods and compositions for modulating protein expression. In particular, the invention features methods and compositions for increasing protein expression in a cell by delivering to the cell a composition including an mRNA encoding a polypeptide and one or more oligonucleotides, wherein each of the one or more oligonucleotides includes a region of linked nucleotides complimentary to a portion of the sequence of the mRNA. The methods and compositions described herein may be used to modulate gene expression (e.g., increase gene expression), to increase the stability of the mRNA, to decrease the immunogenicity of the mRNA, to enable selective expression (e.g., in a target cell or tissue) of the mRNA, and/or to enable the delivery of two or more mRNAs in a stoichiometric ratio.

公开(公告)号：US20210163928A1

公开(公告)日：2021-06-03

申请号：US17041332

申请日：2019-04-11

Applicant: ModernaTX, Inc.

Inventor： David REID ,  Caroline KÖHRER ,  Ruchi JAIN ,  Melissa J. MOORE ,  Scott DONOVAN ,  Aaron LARSEN ,  Vladimir PRESNYAK

IPC: C12N15/11 ,  A61K48/00 ,  C12Q1/6897

Abstract: The present disclosure provides messenger RNAs (mRNAs) having chemical and/or structural modifications, including RNA elements and/or modified nucleotides, in particular C-rich or CG-rich elements, which provide a desired translational regulatory activity to the mRNA.