专利检索 ap:("Monsanto Technology LLC") AND inv:"Alberto landolino" 第 1 页

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发明申请
Methods and Compositions for Delivering Nucleic Acids to Plant Cells and Regulating Gene Expression 审中-公开

公开(公告)号：US20170130237A1

公开(公告)日：2017-05-11

申请号：US15321969

申请日：2015-06-24

申请人： Monsanto Technology LLC

发明人： Michael Bennett , Bill L. Hendrix , Alberto landolino , Yao Luo , Wei Zheng

IPC分类号： C12N15/82

CPC分类号： C12N15/8206 , A01N43/90 , C12N15/8218 , C12N15/8274 , C12N15/8281 , C12N15/8282 , C12N15/8283 , C12N15/8285 , C12N15/8286

摘要： Transfection of plant cells with dsRNA through foliar application encounters cuticle, cell wall and plasmalemma three major barriers. We developed cationic polymer and sugar based formulations and protocols that can effectively deliver dsRNA into plant cells resulted in gene silencing. This disclosure covers the novel methods to deliver dsRNA into plant suspension cells with ‘one step’ treatment and plant foliar cells with ‘one step’ topical application.