公开(公告)号：US20170130237A1

公开(公告)日：2017-05-11

申请号：US15321969

申请日：2015-06-24

Applicant: Monsanto Technology LLC

Inventor： Michael Bennett ,  Bill L. Hendrix ,  Alberto landolino ,  Yao Luo ,  Wei Zheng

IPC: C12N15/82

CPC classification number: C12N15/8206 ,  A01N43/90 ,  C12N15/8218 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8282 ,  C12N15/8283 ,  C12N15/8285 ,  C12N15/8286

Abstract: Transfection of plant cells with dsRNA through foliar application encounters cuticle, cell wall and plasmalemma three major barriers. We developed cationic polymer and sugar based formulations and protocols that can effectively deliver dsRNA into plant cells resulted in gene silencing. This disclosure covers the novel methods to deliver dsRNA into plant suspension cells with ‘one step’ treatment and plant foliar cells with ‘one step’ topical application.