公开(公告)号：US20150197764A1

公开(公告)日：2015-07-16

申请号：US14632018

申请日：2015-02-26

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Eric D. Aasen ,  Mark Scott Abad ,  Edwards Allen ,  Thandoni Rao Ambika ,  Veena S. Anil ,  Alice Clara Augustine ,  Stephanie L. Back ,  Pranesh Badami ,  Amarjit Basra ,  Kraig L. Brustad ,  Molian Deng ,  Todd Dezwaan ,  Charles Dietrich ,  Stephen M. Duff ,  Karen K. Gabbert ,  Steve He ,  Bill L. Hendrix ,  Hongwu Jia ,  Vidya Kammaradi Ramanath Bhat ,  Balasulojini Karunanandaa ,  Kevin R. Kosola ,  Kempagangaiah Krishnamurthy ,  Ganesh Kumar ,  Saritha V. Kuriakose ,  Shantala Lakkanna ,  Mark E. Leibman ,  Linda Lutfiyya ,  Savitha Madappa ,  Sivalinganna Manjunath ,  Robert J. Meister ,  Bellur Narasimha Prasad ,  Sasha Preuss ,  Qungang Qi ,  Dhanalakshmi Ramachandra ,  Aniruddha Raychaudhuri ,  S. Sangeetha ,  Scott Saracco ,  Chitresh Sharma ,  Vijay K. Sharma ,  Rajani Monnanda Somaiah ,  Padmini Sudarshana ,  Rebecca L. Thompson-Mize ,  Srikanth Venkatachalayya ,  Tyamagondlu V. Venkatesh ,  Huai Wang ,  Jingrui Wu ,  Xiaoyun Wu ,  Xiaofeng Sean Yang ,  Qin Zeng ,  Jianmin Zhao

IPC: C12N15/82

CPC classification number: C12N15/8251 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8275 ,  Y02A40/146

Abstract: This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced trait.

Abstract translation: 本发明提供具有用于表达蛋白质的重组DNA的转基因植物细胞，其可用于赋予转基因作物植物增强的农艺性状。 本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率， 种子蛋白和增强种子油。 还公开了用于制备具有增强特征的转基因种子和植物的方法。

公开(公告)号：US11807857B2

公开(公告)日：2023-11-07

申请号：US15321969

申请日：2015-06-24

Applicant: Monsanto Technology LLC

Inventor： Michael Bennett ,  Bill L. Hendrix ,  Alberto Iandolino ,  Yao Luo ,  Wei Zheng

IPC: C12N15/82

CPC classification number: C12N15/8206 ,  C12N15/8218 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8282 ,  C12N15/8283 ,  C12N15/8285 ,  C12N15/8286

Abstract: Transfection of plant cells with dsRNA through foliar application encounters cuticle, cell wall and plasmalemma three major barriers. We developed cationic polymer and sugar based formulations and protocols that can effectively deliver dsRNA into plant cells resulted in gene silencing. This disclosure covers the novel methods to deliver dsRNA into plant suspension cells with ‘one step’ treatment and plant foliar cells with ‘one step’ topical application.

公开(公告)号：US20140137278A1

公开(公告)日：2014-05-15

申请号：US14044647

申请日：2013-10-02

Applicant: Monsanto Technology LLC

Inventor： Muhammad Bhatti ,  Roy G. Cantrell ,  Seungho Cho ,  Issa Coulibaly ,  Bill L. Hendrix ,  Don Lee Keim ,  Kunsheng Wu ,  Jinhua Xiao

IPC: C12N15/82

CPC classification number: A01H1/04 ,  A01H5/10 ,  C07K14/415

Abstract: The present invention relates to methods for breeding cotton plants containing a recombination event such that quantitative trait loci (“QTL”) on chromosome 11 are present, associated with resistance to both root-knot nematode (“RKN”) and reniform nematode (“REN”) infection (“stacked nematode resistance”). The invention further provides germplasm and the use of germplasm containing such stacked nematode resistance as a source of nematode resistance alleles for introgression into elite germplasm in a breeding program, thus producing novel elite germplasm comprising the stacked nematode resistance trait.

Abstract translation: 本发明涉及用于育种含有重组事件的棉花植物的方法，使得存在染色体11上的数量性状位点（“QTL”），其与根结线虫（“RKN”）和肾状线虫（“REN”）的抗性相关 “）感染（”堆叠的线虫抗性“）。 本发明进一步提供种质和使用含有这种堆叠的线虫抗性的种质作为线虫抗性等位基因的来源，用于在育种程序中渗入精英种质，从而产生包含叠层的线虫抗性性状的新颖的种质。

公开(公告)号：US20180237793A1

公开(公告)日：2018-08-23

申请号：US15827886

申请日：2017-11-30

Applicant: Monsanto Technology LLC

Inventor： Eric D. Aasen ,  Mark Scott Abad ,  Edwards Allen ,  Thandoni Rao Ambika ,  Veena S. Anil ,  Alice Clara Augustine ,  Stephanie L. Back ,  Pranesh Badami ,  Amarjit Basra ,  Kraig L. Brustad ,  Molian Deng ,  Todd Dezwaan ,  Charles Dietrich ,  Stephen M. Duff ,  Karen K. Gabbert ,  Steve He ,  Bill L. Hendrix ,  Hongwu Jia ,  Vidya Kammaradi Ramanath Bhat ,  Balasulojini Karunanandaa ,  Kevin R. Kosola ,  Kempagangaiah Krishnamurthy ,  Ganesh Kumar ,  Saritha V. Kuriakose ,  Shantala Lakkanna ,  Mark E. Leibman ,  Linda Lutfiyya ,  Savitha Madappa ,  Sivalinganna Manjunath ,  Robert J. Meister ,  Bellur Narasimha Prasad ,  Sasha Preuss ,  Qungang Qi ,  Dhanalakshmi Ramachandra ,  Aniruddha Raychaudhuri ,  S. Sangeetha ,  Scott Saracco ,  Chitresh Sharma ,  Vijay K. Sharma ,  Monnanda S. Rajani ,  Padmini Sudarshana ,  Rebecca L. Thompson-Mize ,  Srikanth Venkatachalayya ,  Tyamagondlu V. Venkatesh ,  Huai Wang ,  Jingrui Wu ,  Xiaoyun Wu ,  Xiaofeng Sean Yang ,  Qin Zeng ,  Jianmin Zhao

IPC: C12N15/82

CPC classification number: C12N15/8251 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8275 ,  Y02A40/146

Abstract: This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced trait.

公开(公告)号：US20170130237A1

公开(公告)日：2017-05-11

申请号：US15321969

申请日：2015-06-24

Applicant: Monsanto Technology LLC

Inventor： Michael Bennett ,  Bill L. Hendrix ,  Alberto landolino ,  Yao Luo ,  Wei Zheng

IPC: C12N15/82

CPC classification number: C12N15/8206 ,  A01N43/90 ,  C12N15/8218 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8282 ,  C12N15/8283 ,  C12N15/8285 ,  C12N15/8286

Abstract: Transfection of plant cells with dsRNA through foliar application encounters cuticle, cell wall and plasmalemma three major barriers. We developed cationic polymer and sugar based formulations and protocols that can effectively deliver dsRNA into plant cells resulted in gene silencing. This disclosure covers the novel methods to deliver dsRNA into plant suspension cells with ‘one step’ treatment and plant foliar cells with ‘one step’ topical application.