公开(公告)号：US11225671B2

公开(公告)日：2022-01-18

申请号：US16370546

申请日：2019-03-29

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Rico A. Caldo ,  Shunhong Dai ,  Karen Gabbert ,  Alexander Goldshmidt ,  Miya D. Howell ,  Balasulojini Karunanandaa ,  Sivalinganna Manjunath ,  Bradley W. McDill ,  Daniel J. Ovadya ,  Sasha Preuss ,  Elena A. Rice ,  Beth Savidge ,  Vijay K. Sharma

IPC: C12N15/82 ,  C07K14/41 ,  C07K14/415

Abstract: The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.

公开(公告)号：US20230159946A1

公开(公告)日：2023-05-25

申请号：US18059292

申请日：2022-11-28

Applicant: Monsanto Technology LLC

Inventor： Brent BROWER-TOLAND ,  Shunhong Dai ,  Karen Gabbert ,  Alexander Goldshmidt ,  Miya Howell ,  Brad Mcdill ,  Dan Ovadya ,  Beth Savidge ,  Vijay Sharma

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/827 ,  C12N15/8261 ,  C07K14/415 ,  C12N15/8218 ,  Y02A40/146

Abstract: The present invention provides recombinant DNA constructs, vectors and molecules useful for attenuating and/or refining the expression of a florigenic FT gene or transgene using targeting sequences of small RNA molecules. Transgenic plants, plant cells and tissues, and plant parts comprising the recombinant constructs, vectors, and molecules are also provided. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant via suppression, relative to a control or wild type plant. Methods are further provided for introducing the recombinant DNA constructs, vectors, and molecules into a plant, and planting transgenic plants in the field including at higher densities. Transgenic plants of the present invention may provide greater yield potential than wild type or control plants.

公开(公告)号：US20190300890A1

公开(公告)日：2019-10-03

申请号：US16370546

申请日：2019-03-29

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Rico A. Caldo ,  Shunhong Dai ,  Karen Gabbert ,  Alexander Goldshmidt ,  Miya D. Howell ,  Balasulojini Karunanandaa ,  Sivalinganna Manjunath ,  Bradley W. McDill ,  Daniel J. Ovadya ,  Sasha Preuss ,  Elena A. Rice ,  Beth Savidge ,  Vijay K. Sharma

IPC: C12N15/82 ,  C07K14/415

Abstract: The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.

公开(公告)号：US12110495B2

公开(公告)日：2024-10-08

申请号：US16370561

申请日：2019-03-29

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Rico A. Caldo ,  Shunhong Dai ,  Karen Gabbert ,  Alexander Goldshmidt ,  Miya D. Howell ,  Balasulojini Karunanandaa ,  Sivalinganna Manjunath ,  Bradley W. McDill ,  Daniel J. Ovadya ,  Sasha Preuss ,  Elena A. Rice ,  Beth Savidge ,  Vijay K. Sharma

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/827 ,  C07K14/415 ,  C12N15/8261 ,  Y02A40/146

Abstract: The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.

公开(公告)号：US11414670B2

公开(公告)日：2022-08-16

申请号：US16847283

申请日：2020-04-13

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Edwards M. Allen ,  Jayanand Boddu ,  Charles R. Dietrich ,  Alexander Goldshmidt ,  Miya Howell ,  Kevin R. Kosola ,  Anil Neelam ,  Thomas L. Slewinski ,  Tyamagondlu V. Venkatesh ,  Huai Wang ,  Sivalinganna Manjunath ,  Linda Rymarquis

IPC: C12N15/82

Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.

公开(公告)号：US20220090105A1

公开(公告)日：2022-03-24

申请号：US17543811

申请日：2021-12-07

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Rico A. Caldo ,  Shunhong Dai ,  Karen Gabbert ,  Alexander Goldshmidt ,  Miya D. Howell ,  Balasulojini Karunanandaa ,  Sivalinganna Manjunath ,  Bradley W. McDill ,  Daniel J. Ovadya ,  Sasha Preuss ,  Elena A. Rice ,  Beth Savidge ,  Vijay K. Sharma

IPC: C12N15/82 ,  C07K14/415

Abstract: The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.

公开(公告)号：US11555201B2

公开(公告)日：2023-01-17

申请号：US15787359

申请日：2017-10-18

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Brent Brower-Toland ,  Shunhong Dai ,  Karen Gabbert ,  Alexander Goldshmidt ,  Miya Howell ,  Brad McDill ,  Dan Ovadya ,  Beth Savidge ,  Vijay Sharma

IPC: C12N15/82 ,  C07K14/415

Abstract: The present invention provides recombinant DNA constructs, vectors and molecules useful for attenuating and/or refining the expression of a florigenic FT gene or transgene using targeting sequences of small RNA molecules. Transgenic plants, plant cells and tissues, and plant parts comprising the recombinant constructs, vectors, and molecules are also provided. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant via suppression, relative to a control or wild type plant. Methods are further provided for introducing the recombinant DNA constructs, vectors, and molecules into a plant, and planting transgenic plants in the field including at higher densities. Transgenic plants of the present invention may provide greater yield potential than wild type or control plants.

公开(公告)号：US11319550B2

公开(公告)日：2022-05-03

申请号：US16847244

申请日：2020-04-13

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Edwards M. Allen ,  Jayanand Boddu ,  Charles R. Dietrich ,  Alexander Goldshmidt ,  Miya Howell ,  Kevin R. Kosola ,  Anil Neelam ,  Thomas L. Slewinski ,  Tyamagondlu V. Venkatesh ,  Huai Wang ,  Sivalinganna Manjunath ,  Linda Rymarquis

IPC: C12N15/82

Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.

公开(公告)号：US20160304891A1

公开(公告)日：2016-10-20

申请号：US15131987

申请日：2016-04-18

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Rico A. Caldo ,  Shunhong Dai ,  Karen Gabbert ,  Alexander Goldshmidt ,  Miya D. Howell ,  Balasulojini Karunanandaa ,  Sivalinganna Manjunath ,  Bradley W. McDill ,  Daniel J. Ovadya ,  Sasha Preuss ,  Elena A. Rice ,  Beth Savidge ,  Vijay K. Sharma

IPC: C12N15/82 ,  A01G1/00

CPC classification number: C12N15/827 ,  C07K14/415 ,  C12N15/8261 ,  Y02A40/146

Abstract: The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.

Abstract translation: 本发明提供了重组DNA构建体，载体和分子，其包含编码与营养阶段启动子可操作地连接的产花型FT蛋白的多核苷酸序列，其也可以是分生组织优选的或分生组织特异性启动子。 进一步提供了转基因植物，植物细胞和组织以及植物部分，其包含编码花粉蛋白FT蛋白的多核苷酸序列。 包含富含花青型FT转基因的转基因植物相对于对照或野生型植物，可以在转基因植物上，尤其是植物主茎上的每个节点产生更多的铃，长角果，果实，坚果或荚果。 进一步提供用于将花生FT转基因导入植物的方法，以及在该领域种植转基因FT植物，包括以较高的密度。 因此，本发明的转基因植物可以提供比野生型植物更大的产量潜力，并且由于其改变的植物结构，可以以更高的密度种植。

公开(公告)号：US10724047B2

公开(公告)日：2020-07-28

申请号：US15679699

申请日：2017-08-17

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Edwards M. Allen ,  Jayanand Boddu ,  Charles R. Dietrich ,  Alexander Goldshmidt ,  Miya Howell ,  Kevin R. Kosola ,  Sivalinganna Manjunath ,  Anil Neelam ,  Linda Rymarquis ,  Thomas L. Slewinski ,  Tyamagondlu V. Venkatesh ,  Huai Wang

IPC: C12N15/82 ,  C12N15/113

Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.