公开(公告)号：US12110495B2

公开(公告)日：2024-10-08

申请号：US16370561

申请日：2019-03-29

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Rico A. Caldo ,  Shunhong Dai ,  Karen Gabbert ,  Alexander Goldshmidt ,  Miya D. Howell ,  Balasulojini Karunanandaa ,  Sivalinganna Manjunath ,  Bradley W. McDill ,  Daniel J. Ovadya ,  Sasha Preuss ,  Elena A. Rice ,  Beth Savidge ,  Vijay K. Sharma

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/827 ,  C07K14/415 ,  C12N15/8261 ,  Y02A40/146

Abstract: The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.

公开(公告)号：US11414670B2

公开(公告)日：2022-08-16

申请号：US16847283

申请日：2020-04-13

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Edwards M. Allen ,  Jayanand Boddu ,  Charles R. Dietrich ,  Alexander Goldshmidt ,  Miya Howell ,  Kevin R. Kosola ,  Anil Neelam ,  Thomas L. Slewinski ,  Tyamagondlu V. Venkatesh ,  Huai Wang ,  Sivalinganna Manjunath ,  Linda Rymarquis

IPC: C12N15/82

Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.

公开(公告)号：US20220090105A1

公开(公告)日：2022-03-24

申请号：US17543811

申请日：2021-12-07

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Rico A. Caldo ,  Shunhong Dai ,  Karen Gabbert ,  Alexander Goldshmidt ,  Miya D. Howell ,  Balasulojini Karunanandaa ,  Sivalinganna Manjunath ,  Bradley W. McDill ,  Daniel J. Ovadya ,  Sasha Preuss ,  Elena A. Rice ,  Beth Savidge ,  Vijay K. Sharma

IPC: C12N15/82 ,  C07K14/415

Abstract: The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.

公开(公告)号：US20190300890A1

公开(公告)日：2019-10-03

申请号：US16370546

申请日：2019-03-29

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Rico A. Caldo ,  Shunhong Dai ,  Karen Gabbert ,  Alexander Goldshmidt ,  Miya D. Howell ,  Balasulojini Karunanandaa ,  Sivalinganna Manjunath ,  Bradley W. McDill ,  Daniel J. Ovadya ,  Sasha Preuss ,  Elena A. Rice ,  Beth Savidge ,  Vijay K. Sharma

IPC: C12N15/82 ,  C07K14/415

Abstract: The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.

公开(公告)号：US20160257968A1

公开(公告)日：2016-09-08

申请号：US15028381

申请日：2014-10-01

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Cara L. Griffith ,  Abha Khandelwal, JR. ,  Paul J. Loida ,  Elena A. Rice ,  Rebecca L. Thompson ,  Sivalinganna Manjunath

IPC: C12N15/82

Abstract: A recombinant DNA construct is disclosed. When the recombinant DNA construct is expressed in a plant or a plant cell, endogenous HD-Zip class II proteins become less able to repress DNA transcription of the genes they typically regulate. The recombinant DNA construct can be expressed in plant cells to produce plants with enhanced phenotypes. Methods of making transgenic plants comprising the recombinant DNA construct, and plants produced thereby are also disclosed.

Abstract translation: 公开了重组DNA构建体。 当重组DNA构建体在植物或植物细胞中表达时，内源性HD-Zip II类蛋白质变得不能抑制它们通常调节的基因的DNA转录。 重组DNA构建体可以在植物细胞中表达以产生具有增强表型的植物。 还公开了制备包含重组DNA构建体的转基因植物以及由此产生的植物的方法。

公开(公告)号：US20150197764A1

公开(公告)日：2015-07-16

申请号：US14632018

申请日：2015-02-26

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Eric D. Aasen ,  Mark Scott Abad ,  Edwards Allen ,  Thandoni Rao Ambika ,  Veena S. Anil ,  Alice Clara Augustine ,  Stephanie L. Back ,  Pranesh Badami ,  Amarjit Basra ,  Kraig L. Brustad ,  Molian Deng ,  Todd Dezwaan ,  Charles Dietrich ,  Stephen M. Duff ,  Karen K. Gabbert ,  Steve He ,  Bill L. Hendrix ,  Hongwu Jia ,  Vidya Kammaradi Ramanath Bhat ,  Balasulojini Karunanandaa ,  Kevin R. Kosola ,  Kempagangaiah Krishnamurthy ,  Ganesh Kumar ,  Saritha V. Kuriakose ,  Shantala Lakkanna ,  Mark E. Leibman ,  Linda Lutfiyya ,  Savitha Madappa ,  Sivalinganna Manjunath ,  Robert J. Meister ,  Bellur Narasimha Prasad ,  Sasha Preuss ,  Qungang Qi ,  Dhanalakshmi Ramachandra ,  Aniruddha Raychaudhuri ,  S. Sangeetha ,  Scott Saracco ,  Chitresh Sharma ,  Vijay K. Sharma ,  Rajani Monnanda Somaiah ,  Padmini Sudarshana ,  Rebecca L. Thompson-Mize ,  Srikanth Venkatachalayya ,  Tyamagondlu V. Venkatesh ,  Huai Wang ,  Jingrui Wu ,  Xiaoyun Wu ,  Xiaofeng Sean Yang ,  Qin Zeng ,  Jianmin Zhao

IPC: C12N15/82

CPC classification number: C12N15/8251 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8275 ,  Y02A40/146

Abstract: This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced trait.

Abstract translation: 本发明提供具有用于表达蛋白质的重组DNA的转基因植物细胞，其可用于赋予转基因作物植物增强的农艺性状。 本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率， 种子蛋白和增强种子油。 还公开了用于制备具有增强特征的转基因种子和植物的方法。

公开(公告)号：US20230354762A1

公开(公告)日：2023-11-09

申请号：US18190739

申请日：2023-03-27

Applicant: Monsanto Technology LLC

Inventor： Steven Beach ,  Sivalinganna Manjunath ,  Linda Rymarquis ,  Thomas L. Slewinski ,  Xiaoyun Wu

IPC: A01H6/46 ,  C12N15/82 ,  A01H5/10 ,  C12N9/22

CPC classification number: A01H6/4684 ,  A01H5/10 ,  C12N9/22 ,  C12N15/8262 ,  C12N2310/20

Abstract: Provided are compositions and methods for reducing, disrupting, or altering ZmDA1 activity in corn plants. Methods and compositions are also provided for producing modifications in the ZmDA1 gene through mutagenesis and/or editing. Modified plant cells and plants having a modification in the ZmDA1 gene are further provided comprising improved characteristics, such as increased yield.

公开(公告)号：US11225671B2

公开(公告)日：2022-01-18

申请号：US16370546

申请日：2019-03-29

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Rico A. Caldo ,  Shunhong Dai ,  Karen Gabbert ,  Alexander Goldshmidt ,  Miya D. Howell ,  Balasulojini Karunanandaa ,  Sivalinganna Manjunath ,  Bradley W. McDill ,  Daniel J. Ovadya ,  Sasha Preuss ,  Elena A. Rice ,  Beth Savidge ,  Vijay K. Sharma

IPC: C12N15/82 ,  C07K14/41 ,  C07K14/415

Abstract: The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.

公开(公告)号：US10829773B2

公开(公告)日：2020-11-10

申请号：US15028381

申请日：2014-10-01

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Cara L. Griffith ,  Abha Khandelwal ,  Paul J. Loida ,  Elena A. Rice ,  Rebecca L. Thompson ,  Sivalinganna Manjunath

IPC: C12N15/82

Abstract: A recombinant DNA construct is disclosed. When the recombinant DNA construct is expressed in a plant or a plant cell, endogenous HD-Zip class II proteins become less able to repress DNA transcription of the genes they typically regulate. The recombinant DNA construct can be expressed in plant cells to produce plants with enhanced phenotypes. Methods of making transgenic plants comprising the recombinant DNA construct, and plants produced thereby are also disclosed.

公开(公告)号：US20160237447A1

公开(公告)日：2016-08-18

申请号：US15027777

申请日：2014-10-06

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Mark Scott Abad ,  Edwards M Allen ,  Veena S Anil ,  Alice Clara Augustine ,  Isaac R Banks ,  Prasanna R Bhat ,  Jaishree M Chittoor-Vijayanath ,  Paul S Chomet ,  Molian Deng ,  Stephen Duff ,  Barry S Goldman ,  Sergey Ivashuta ,  Balasulojini Karunanandaa ,  Linda Lutfiyya ,  Sivalinganna Manjunath ,  Anil Neelam ,  Monnanda S Rajani ,  G Ramamohan ,  Chitresh Sharma ,  Char Shobha ,  Tyamagondlu V Venkatesh ,  Kammaradi R Vidya ,  Jianmin Zhao

IPC: C12N15/82 ,  C12N15/113 ,  A01H1/02

CPC classification number: C12N15/8241 ,  A01H1/02 ,  C12N15/113 ,  C12N15/8261 ,  C12N15/8271 ,  C12N2310/141

Abstract: This disclosure provides transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing hybrid seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.