公开(公告)号：US20230057290A1

公开(公告)日：2023-02-23

申请号：US17818913

申请日：2022-08-10

Applicant: Monsanto Technology LLC

Inventor： Brian J. Martinell ,  Anna Mary O'Keefe ,  David Alan Somers ,  Edward James Williams ,  Xudong Ye

IPC: C12N15/82

Abstract: Methods and compositions for plastid transformation and regeneration or development of transplastomic plants are provided. Embryo explants may be excised from seeds, and their meristematic tissue may be transformed directly without initiation of any callus phase before and/or after transformation. The present methods may be performed with fewer culturing steps relative to conventional methods, thereby enabling more rapid and efficient production of targeted transplastomic events in plants.

公开(公告)号：US20160264983A1

公开(公告)日：2016-09-15

申请号：US15015968

申请日：2016-02-04

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： BRIAN J. MARTINELL ,  Anna Mary O'Keefe ,  David Alan Somers ,  Edward James Williams ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8214 ,  C12N15/8207 ,  C12N15/8213

Abstract: Methods and compositions for plastid transformation and regeneration or development of transplastomic plants are provided. Embryo explants may be excised from seeds, and their meristematic tissue may be transformed directly without initiation of any callus phase before and/or after transformation. The present methods may be performed with fewer culturing steps relative to conventional methods, thereby enabling more rapid and efficient production of targeted transplastomic events in plants.

Abstract translation: 提供了质体转化和转基因植物再生或发育的方法和组合物。 胚外植体可以从种子中切除，并且它们的分生组织可以在转化前后和/或之后直接转化而不引发任何愈伤组织相。 本方法可以相对于常规方法进行较少的培养步骤，从而使得能够更快速和有效地生产植物中靶向的转基因组学事件。