公开(公告)号：US20250137002A1

公开(公告)日：2025-05-01

申请号：US18983993

申请日：2024-12-17

Applicant: Monsanto Technology LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  A01H4/00 ,  A01H6/20 ,  A01H6/46 ,  A01H6/54 ,  A01H6/60 ,  C12N1/20 ,  C12N5/00 ,  C12N5/04 ,  C12N9/10 ,  C12R1/41 ,  G01N33/00

Abstract: The present invention relates to methods and compositions for transforming soybean, corn. cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US12157893B2

公开(公告)日：2024-12-03

申请号：US17523755

申请日：2021-11-10

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： John Bradley ,  Larry A. Gilbertson ,  Ervin Nagy ,  Christine Shyu ,  Peizhen Yang ,  Xudong Ye

IPC: C12N15/82

Abstract: The present disclosure relates to compositions and methods for improving site-directed integration frequency in the genome of plant cells.

公开(公告)号：US11859191B2

公开(公告)日：2024-01-02

申请号：US17817196

申请日：2022-08-03

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski ,  Elysia Krieger ,  Ervin Nagy ,  Krishnakumar Sridharan ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8205 ,  C12N2310/20 ,  C12N2800/80

Abstract: This disclosure is related to plant-optimized recombinant nucleic acids encoding Cpf1 and their use in planta. Also disclosed are compositions, expression cassettes, and plant cells comprising the recombinant nucleic acids as well as methods and kits for modifying a target sequence in a plant genome using the recombinant nucleic acids.

公开(公告)号：US11629357B2

公开(公告)日：2023-04-18

申请号：US17079348

申请日：2020-10-23

Applicant: Monsanto Technology LLC

Inventor： Xudong Ye ,  Larry A. Gilbertson ,  Shihshieh Huang ,  Susan J. Johnson ,  Michael W. Petersen ,  David Walters ,  Paul S. Chomet

IPC: C12N15/82 ,  C12N9/10

Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.

公开(公告)号：US09464295B2

公开(公告)日：2016-10-11

申请号：US14295096

申请日：2014-06-03

Applicant: Monsanto Technology LLC

Inventor： Larry A. Gilbertson ,  Xudong Ye

IPC: C12N15/84 ,  C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8202

Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.

公开(公告)号：US09365859B2

公开(公告)日：2016-06-14

申请号：US14298739

申请日：2014-06-06

Applicant: Monsanto Technology LLC

Inventor： Xudong Ye ,  Junjiang Shen ,  Edward Williams

IPC: C12N15/82 ,  C12R1/41

CPC classification number: C12N15/8202 ,  C12N15/8205 ,  C12R1/41 ,  G01N33/5097

Abstract: The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.

Abstract translation: 本发明涉及用于根瘤菌介导的植物细胞遗传转化的方法，包括大豆，卡诺拉油菜，玉米和棉花细胞。 这些包括依赖于VirD2和与VirD2无关的方法。 使用的细菌种类包括根瘤菌属，中华根瘤菌属（Sinorhizobium sp。）和中生根瘤菌属（Mesorhizobium sp。 还公开了用于这种转化的载体。

公开(公告)号：US08872000B2

公开(公告)日：2014-10-28

申请号：US13899452

申请日：2013-05-21

Applicant: Monsanto Technology LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  C12N15/62 ,  A01H1/04 ,  G01N33/00 ,  C12N5/04 ,  A01H4/00

CPC classification number: C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

Abstract translation: 本发明涉及使用壮观霉素作为转化外植体的选择剂转化大豆，玉米，棉花或油菜外植体的方法和组合物。 该方法还可以包括在转化和再生过程中用细胞分裂素处理外植体。

公开(公告)号：US12275944B2

公开(公告)日：2025-04-15

申请号：US18501949

申请日：2023-11-03

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski ,  Elysia Krieger ,  Ervin Nagy ,  Krishnakumar Sridharan ,  Xudong Ye

IPC: C12N15/82

Abstract: This disclosure is related to plant-optimized recombinant nucleic acids encoding Cpf1 and their use in planta. Also disclosed are compositions, expression cassettes, and plant cells comprising the recombinant nucleic acids as well as methods and kits for modifying a target sequence in a plant genome using the recombinant nucleic acids.

公开(公告)号：US10907167B2

公开(公告)日：2021-02-02

申请号：US16393833

申请日：2019-04-24

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  C12N15/84 ,  C12N15/62 ,  A01H1/04 ,  C12N9/10 ,  A01H4/00 ,  A01H6/20 ,  A01H6/46 ,  A01H6/54 ,  A01H6/60 ,  C12N5/00 ,  C12R1/41

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US20190211346A1

公开(公告)日：2019-07-11

申请号：US16242887

申请日：2019-01-08

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Xudong Ye ,  Larry A. Gilbertson ,  Michael W. Petersen

IPC: C12N15/82

Abstract: Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.